6个紫花苜蓿品种根系形态结构对低磷胁迫的响应

doi:10.11733/j.issn.1007-0435.2021.11.015

草地学报 ›› 2021, Vol. 29 ›› Issue (11): 2494-2504.DOI: 10.11733/j.issn.1007-0435.2021.11.015

6个紫花苜蓿品种根系形态结构对低磷胁迫的响应

潘新雅^1,2, 李军保³, 陈阳², 王鹏飞^1,2, 卫先伟^1,2, 李瑞¹, 刘佳茜¹, 郑智龙¹, 徐炳成^1,2,4, 王智^1,2,4

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西北农林科技大学黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100;
3. 陕西省林业科学院, 陕西西安 710000;
4. 中国科学院水利部水土保持研究所, 陕西杨凌 712100

收稿日期:2021-04-22 修回日期:2021-05-19 出版日期:2021-11-15 发布日期:2021-12-06
通讯作者: 王智,E-mail:wangzhi712@nwafu.edu.cn
作者简介:潘新雅(1997-),女,河南信阳人,硕士研究生,主要从事牧草逆境生物学研究,E-mail:xypan@nwafu.edu.cn
基金资助:
陕西省自然科学基础研究计划青年项目（2020JQ-264）；陕西省重点研发计划（2019TSLNY03-01）；国家自然科学基金（31700335）共同资助

Response of Root Morphology and Anatomical Structure of Six Alfalfa Cultivars to Phosphorus Deficiency

PAN Xin-ya^1,2, LI Jun-bao³, CHEN Yang², WANG Peng-fei^1,2, WEI Xian-wei^1,2, LI Rui¹, LIU Jia-xi¹, ZHENG Zhi-long¹, XU Bing-cheng^1,2,4, WANG Zhi^1,2,4

1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
3. Shaanxi Academy of Forestry, Xian, Shaanxi Province 710000, China;
4. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2021-04-22 Revised:2021-05-19 Online:2021-11-15 Published:2021-12-06

摘要/Abstract

摘要： 为明确低磷胁迫下紫花苜蓿（Medicago sativa L.）根系形态结构的响应差异对磷效率的影响，以6个紫花苜蓿品种为材料，采用营养液砂培法，测定两种磷水平（对照：0.5 mmol·L^-1；低磷：0.05 mmol·L^-1）下苜蓿根系形态、解剖结构和磷效率。结果表明：低磷胁迫下，各苜蓿品种的根系长度、表面积、导管直径、中柱直径及其占根直径比例均降低，其余根系性状则呈现不同的变化趋势。低磷胁迫抑制了各苜蓿品种的生长和磷吸收；除‘赛迪’的磷利用效率显著增加外，其余5个苜蓿品种的磷利用效率均显著降低。‘赛迪’主要通过增加根冠比和磷利用效率，表现出较强的耐低磷能力。总体表明，根系长度、表面积、体积、导管直径、中柱直径及其占根直径比例是苜蓿根系响应低磷胁迫的主要指标，其中根系长度和中柱直径占根直径比例分别是影响6个苜蓿品种磷吸收和利用效率最关键的根系性状。

关键词: 根系形态, 根系解剖结构, 磷吸收, 紫花苜蓿, 低磷胁迫

Abstract: This study was aimed to clarify the effects of phosphorus (P) efficiency on the difference in root morphology and anatomical structure of alfalfa. Root morphology,root anatomical structure and P efficiency of six alfalfa (Medicago sativa L.) cultivars were investigated under two P supply levels (Control:0.5 mmol·L^-1;P deficiency:0.05 mmol·L^-1) via nutrient solution sand culture method. Results showed that the root length,surface area,vessel diameter,stele diameter and ratio of stele diameter to root diameter all decreased,while the other root morphology and anatomical structure traits showed variable response characters to P deficiency in six alfalfa cultivars. P deficiency generated evident inhibitory effects on plant growth and P uptake efficiency of six alfalfa cultivars. Compared with control condition,P utilization efficiency of ‘Sidel’ increased significantly,while that of the other five cultivars reduced significantly under P deficiency. Our results implied that ‘Sidel’ performed the highest adaptability to low P environment mainly by increasing its root to shoot ratio and P utilization efficiency. Overall,the root length,surface area and volume,as well as vessel and stele diameter,ratio of stele diameter to root diameter were the main indexes for alfalfa root responding to P deficiency. Among them,root length and ratio of stele diameter to root diameter were the critical root traits affecting P uptake efficiency and P utilization efficiency of six alfalfa cultivars,respectively.

Key words: Root morphology, Root anatomical structure, Phosphorus absorption, Alfalfa, Phosphorus deficiency

中图分类号:

Q945.78

潘新雅, 李军保, 陈阳, 王鹏飞, 卫先伟, 李瑞, 刘佳茜, 郑智龙, 徐炳成, 王智. 6个紫花苜蓿品种根系形态结构对低磷胁迫的响应[J]. 草地学报, 2021, 29(11): 2494-2504.

PAN Xin-ya, LI Jun-bao, CHEN Yang, WANG Peng-fei, WEI Xian-wei, LI Rui, LIU Jia-xi, ZHENG Zhi-long, XU Bing-cheng, WANG Zhi. Response of Root Morphology and Anatomical Structure of Six Alfalfa Cultivars to Phosphorus Deficiency[J]. Acta Agrestia Sinica, 2021, 29(11): 2494-2504.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.11.015

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I11/2494

参考文献

[1] 山仑,徐炳成. 黄土高原半干旱地区建设稳定人工草地的探讨[J]. 草业学报,2009,18(2):1-2
[2] YUAN Z Q,YU K L,EPSTEIN H,et al. Effects of legume species introduction on vegetation and soil nutrient development on abandoned croplands in a semi-arid environment on the Loess Plateau,China[J]. Science of The Total Environment,2016(541):692-700
[3] 张英俊,任继周,王明利,等. 论牧草产业在我国农业产业结构中的地位和发展布局[J]. 中国农业科技导报,2013,15(4):61-71
[4] HOU X L,TIGABU M,ZHANG Y,et al. Root plasticity,whole plant biomass,and nutrient accumulation of Neyraudiarey naudiana in response to heterogeneous phosphorus supply[J]. Journal of Soils and Sediments,2017,17(1):172-180
[5] 李向林,何峰. 苜蓿营养与施肥[M]. 北京:中国农业出版社,2013:118-120
[6] WANG B,XUE S,LIU G B,et al. Changes in soil nutrient and enzyme activities under different vegetations in the Loess Plateau area,Northwest China[J]. Catena,2012(92):186-195
[7] 王冰如. 黄土高原半干旱区苜蓿草地产量及土壤质量对氮磷肥施加的响应[D]. 兰州:兰州大学,2015:4-6
[8] 杨利宁,敖特根·白银,李秋凤,等. 苜蓿根系分泌物对土壤中难溶性磷的影响[J]. 草业科学,2015,32(8):1216-1221
[9] 姜琳琳,韩立思,韩晓日,等. 氮素对玉米幼苗生长、根系形态及氮素吸收利用效率的影响[J]. 植物营养与肥料学报,2011,17(1):247-253
[10] 刘允熙,罗佳佳,雷健,等. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J]. 草地学报,2021,29(5):876-883
[11] JIA X C,LIU P,LYNCH J P. Greater lateral root branching density in maize improves phosphorus acquisition from low phosphorus soil[J]. Journal of Experimental Botany,2018,69(20):4961-4970
[12] 梁颖,石玉,靳琇,等. 低磷胁迫下光质对番茄幼苗生长及生理抗性的影响[J]. 中国农学通报,2020,36(1):56-61
[13] 陶茸,师尚礼,张翠梅,等. 外源香豆素和咖啡酸对紫花苜蓿根系形态建成与解剖结构的影响[J]. 草地学报,2019,27(2):404-412
[14] BAUW D P,VANDAMME E,LUPEMBE A,et al. Anatomical root responses of rice to combined phosphorus and water stress-relations to tolerance and breeding opportunities[J]. Functional Plant Biology,2019,46(11):1009-1022
[15] POST J A,LYNCH J P. Theoretical evidence for the functional benefit of root cortical aerenchyma in soils with low phosphorus availability[J]. Annals of Botany,2010,107(5):829-841
[16] 李丹竹,曾宁波,张志飞,等. 渍水胁迫对不同磷水平下紫花苜蓿根系生长的影响[J]. 草地学报,2020,28(6):1563-1571
[17] 任立飞,张文浩,李衍素. 低磷胁迫对黄花苜蓿生理特性的影响[J]. 草业学报,2012,21(3):242-249
[18] 高艳,田秋英,石凤翎,等. 黄花苜蓿与蒺藜苜蓿对土壤低磷胁迫适应策略的比较研究[J]. 植物生态学报,2011,35(6):632-640
[19] WANG Z,KE Q,KIM M D,et al. Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance[J]. Plos one,2015,10(5):e0126050
[20] 张咏梅,白小明,田彥锋,等. 4种观赏草叶片解剖结构的观察及其对环境的适应性分析[J]. 草地学报,2019,27(5):1377-1383
[21] QIAO S,FANG Y,WU A J,et al. Dissecting root trait variability in maize genotypes using the semi-hydroponic phenotyping platform[J]. Plant and Soil,2019,439(1-2):75-90
[22] 鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社,2000:30-109
[23] FAN J W,DU Y L,TURNER N C,et al. Changes in root morphology and physiology to limited phosphorus and moisture in a locally-selected cultivar and an introduced cultivar of Medicago sativa L. growing in alkaline soil[J]. Plant and Soil,2015,392(1-2):215-226
[24] 杨春婷,张永清,马星星,等. 苦荞耐低磷基因型筛选及评价指标的鉴定[J]. 应用生态学报,2018,29(9):2997-3007
[25] 郑亚萍,王春晓,郑祖林,等. 磷对花生根系形态特征的影响[J]. 中国油料作物学报,2019,41(4):622-628
[26] 朱天琦,刘晓静,张晓玲. 氮营养调控对紫花苜蓿根系形态及其解剖结构的影响[J]. 草地学报,2016,24(6):1290-1295
[27] YANG Z P,ZHENG S X,NIE L,et al. Effects of long-term winter planted green manure on distribution and storage of organic carbon and nitrogen in water-stable aggregates of reddish paddy soil under a double-rice cropping system[J]. Journal of Integrative Agriculture,2014,13(8):1772-1781
[28] SINGH D K,SALE P W G. Growth and potential conductivity of white clover roots in dry soil with increasing phosphorus supply and defoliation frequency[J]. Agronomy Journal,2000,92(5):868-874
[29] TOMBESI S,JOHNSON R S,DAY K R,et al. Relationships between xylem vessel characteristics,calculated axial hydraulic conductance and size-controlling capacity of peach rootstocks[J]. Annals of Botany,2010,105(2):327-331
[30] ZHU J,BROWN K M,LYNCH J P. Root cortical aerenchyma improves the drought tolerance of maize (Zea mays L.)[J]. Plant cell and Environment,2010,33(5):740-749
[31] LYNCH J P. Root phenes that reduce the metabolic costs of soil exploration:opportunities for 21 st century agriculture[J]. Plant,Cell and Environment,2014,38(9):1775-1784
[32] 高静. 小麦苗期根的分枝规律与解剖结构[D]. 北京:中国农业大学,2004:35
[33] BAKER A,CEASAR S A,PALMER A J,et al. Replace,reuse,recycle:Improving the sustainable use of phosphorus by plants[J]. Journal of Experimental Botany,2015,66(12):3523-3540

6个紫花苜蓿品种根系形态结构对低磷胁迫的响应

Response of Root Morphology and Anatomical Structure of Six Alfalfa Cultivars to Phosphorus Deficiency

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王静, 刘晓静, 程甜甜, 童长春, 汪雪. 不同氮效率紫花苜蓿叶特征及其产量效应研究[J]. 草地学报, 2021, 29(9): 1941-1949.
[2]	孟宣辰, 马鹏程, 马杰, 段珍, 韩阳阳, 张吉宇. 黄土高原半干旱区紫花苜蓿覆膜垄沟和施肥效应研究[J]. 草地学报, 2021, 29(9): 2098-2106.
[3]	陈雷, 暴雪艳, 郭刚, 霍文婕, 李清宏, 许庆方, 王聪, 刘强. 单宁酸和乳酸菌对紫花苜蓿青贮品质和体外瘤胃发酵的影响[J]. 草地学报, 2021, 29(8): 1853-1858.
[4]	李春萍, 邱轶辉, 夏厚胤, 谭璐娜, 许环宇, 张瀚文, 高景慧. 紫花苜蓿多父本F₁代低蒸腾及产量性状的隶属函数分析[J]. 草地学报, 2021, 29(7): 1416-1422.
[5]	李天琦, 林志玲, 卢轩, 黄佳媛, 杨宁, 沃野, 高凯, 柳小妮, 王显国. 灌溉量对科尔沁沙地紫花苜蓿土壤速效养分分布及淋失的影响[J]. 草地学报, 2021, 29(7): 1454-1461.
[6]	黄杰, 张郎织, 邢玉芬, 邹晓燕, 刘国道, 虞道耿, 陈志坚. 低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响[J]. 草地学报, 2021, 29(7): 1462-1468.
[7]	李鹏珍, 赵得琴, 邓波, 杨军, 赵国华, 赵亮. 生物炭与干旱胁迫对接种紫花苜蓿光合效率及生长的影响[J]. 草地学报, 2021, 29(6): 1257-1264.
[8]	王文静, 麻冬梅, 赵丽娟, 马巧利. 2,4-表油菜素内酯对盐胁迫下紫花苜蓿生理指标及根系离子积累的影响[J]. 草地学报, 2021, 29(6): 1363-1368.
[9]	潘新怡, 史昆, 龚攀, 韦宝, 吴欣明, 王赞. 紫花苜蓿花青苷合成的转录组分析[J]. 草地学报, 2021, 29(5): 866-875.
[10]	刘允熙, 罗佳佳, 雷健, 刘国道, 刘攀道. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J]. 草地学报, 2021, 29(5): 876-883.
[11]	杨洁, 单立山, 苏铭, 张正中, 解婷婷, 李毅. 降水量和生长方式对红砂和珍珠根系形态特征的影响研究[J]. 草地学报, 2021, 29(5): 936-945.
[12]	黄荣, 姚博, 张玉娟, 贾倩英, 李向阳, 张宏, 张振粉. 成团泛菌CQ10脂多糖对紫花苜蓿种子萌发及苗期生长的影响[J]. 草地学报, 2021, 29(5): 965-971.
[13]	徐洪雨, 甄莉丽, 李钰莹, 董宽虎, 李向林. 低温干旱环境对紫花苜蓿根颈耐寒性的影响[J]. 草地学报, 2021, 29(4): 724-733.
[14]	李满有, 马忠仁, 王斌, 杨雨琦, 沈笑天, 曹立娟, 李小云, 兰剑. 宁夏引黄灌区2个苜蓿品种不同行比混播模式研究[J]. 草地学报, 2021, 29(4): 798-804.
[15]	侯红雁, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 王忠豪, 郭刚. 同型发酵乳杆菌对低水分紫花苜蓿青贮发酵品质及体外消化率的影响[J]. 草地学报, 2021, 29(4): 835-841.