与蒙古冰草抗旱相关的NAC转录因子生物信息学及其表达分析

doi:10.11733/j.issn.1007-0435.2021.06.007

草地学报 ›› 2021, Vol. 29 ›› Issue (6): 1183-1192.DOI: 10.11733/j.issn.1007-0435.2021.06.007

与蒙古冰草抗旱相关的NAC转录因子生物信息学及其表达分析

范菠菠¹, 张学峰¹, 于卓¹, 赵彦², 马艳红¹

1. 内蒙古农业大学农学院, 内蒙古呼和浩特 010019;
2. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010018

收稿日期:2021-03-19 修回日期:2021-04-19 出版日期:2021-06-15 发布日期:2021-07-05
通讯作者: 马艳红,E-mail:mayanhong80@126.com
作者简介:范菠菠(1995-)，女，汉族，内蒙古呼和浩特人，博士研究生，主要从事牧草遗传育种研究，E-mail：fanbobo19@126.com
基金资助:
国家自然科学基金（31860670）；内蒙古自然科学基金（2018MS03016）资助

Bioinformatics and Expression Analysis of NAC Transcription Factors Related to Drought Resistance of Agropyron mongolicum Keng

FAN Bo-bo¹, ZHANG Xue-feng¹, YU Zhuo¹, ZHAO Yan², MA Yan-hong¹

1. College of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. College of Grassland, Resources Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China

Received:2021-03-19 Revised:2021-04-19 Online:2021-06-15 Published:2021-07-05

摘要/Abstract

摘要： NAC转录因子具有调控植物生长发育及应对非生物逆境胁迫等多种功能。为筛选蒙古冰草（Agropyron mongolicum Keng）抗旱相关的NAC转录因子，本研究基于转录组测序数据，利用生物信息学方法对蒙古冰草NAC转录因子的结构、理化性质、进化关系和保守性等进行分析，同时运用实时荧光定量技术对抗旱相关的2个NAC基因进行差异表达分析。结果表明：筛选出26个蒙古冰草NAC家族成员，其中15个具有完整NAC转录因子结构域，其均属于亲水性蛋白，大部分定位于细胞核；15个蒙古冰草NAC转录因子的蛋白磷酸化位点均含有丝氨酸、苏氨酸和酪氨酸。进化关系比对显示AmNAC100和AmNAC102-2转录因子与已知有抗旱功能的NAC蛋白同源性极高，其二、三级结构主要以无规则卷曲为主，motif1，2，3，8为其共有的保守基序，多序列比对将保守结构域划分为子域A—E。差异表达分析发现AmNAC100和AmNAC102-2基因相对表达量均高于对照（CK），在蒙古冰草干旱胁迫中起正调控作用。

关键词: 蒙古冰草, NAC基因家族, 抗旱, 实时荧光定量

Abstract: NAC transcription factor has many functions such as regulating plant growth and abiotic stress. In order to screen NAC transcription factors related to drought resistance in Agropyron mongolicum Keng,the structure,physical and chemical properties,evolutionary relationship and conservation of NAC transcription factors in Agropyron mongolicum Keng were analyzed by bioinformatics based on transcriptome sequencing data. Then,the differential expression of two NAC genes related to drought resistance was analyzed by qRT-PCR. The results showed that 26 NAC family members were screened out from Agropyron mongolicum Keng,15 of them had complete NAC transcription factor domains,and most of them were located in the nucleus. The protein phosphorylation sites of 15 NAC transcription factors from Agropyron mongolicum Keng contain serine,threonine and tyrosine. Phylogenetic comparison showed that AmNAC100 and AmNAC102-2 proteins had high homology with NAC proteins with known drought resistance function and their secondary and tertiary structures were mainly random coil. Two NAC proteins contain motif 1,2,3,and motif 8,and they contain subdomains A-E of conservative domain. Differential expression analysis showed that the relative expression of AmNAC100 and AmNAC102-2 genes were higher than CK,indicating that they played positive regulatory roles in Agropyron mongolicum Keng drought stress.

Key words: Agropyron mongolicum Keng, NAC gene family, Drought resistance, qRT-PCR

中图分类号:

S543+.9

范菠菠, 张学峰, 于卓, 赵彦, 马艳红. 与蒙古冰草抗旱相关的NAC转录因子生物信息学及其表达分析[J]. 草地学报, 2021, 29(6): 1183-1192.

FAN Bo-bo, ZHANG Xue-feng, YU Zhuo, ZHAO Yan, MA Yan-hong. Bioinformatics and Expression Analysis of NAC Transcription Factors Related to Drought Resistance of Agropyron mongolicum Keng[J]. Acta Agrestia Sinica, 2021, 29(6): 1183-1192.

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与蒙古冰草抗旱相关的NAC转录因子生物信息学及其表达分析

Bioinformatics and Expression Analysis of NAC Transcription Factors Related to Drought Resistance of Agropyron mongolicum Keng

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