草地学报

草地学报 ›› 2022, Vol. 30 ›› Issue (2): 370-378.DOI: 10.11733/j.issn.1007-0435.2022.02.014

• 研究论文 • 上一篇    

根瘤菌拌种对两种苜蓿生长及根际微生物多样性的影响

赵阳安1, 芦光新1, 邓晔2, 王英成1, 马坤1, 颜珲璘1, 张海娟1, 周学丽3, 窦声云3   

  1. 1. 青海大学农牧学院, 青海 西宁 810016;
    2. 中国科学院生态环境研究中心, 北京 100080;
    3. 青海省草原改良试验站, 青海 共和 813000
  • 收稿日期:2021-07-25 修回日期:2021-08-30 发布日期:2022-03-10
  • 通讯作者: 芦光新,E-mail:lugx74@qq.com;邓晔,E-mail:yedeng@rcees.ac.cn
  • 作者简介:赵阳安(1996-),男,汉族,甘肃陇南人,硕士研究生,主要从事草地生态与环境保护研究,E-mail:2093289276@qq.com
  • 基金资助:
    青海省科技厅基础研究面上计划项目(2021-ZJ-915);青海省“昆仑英才·高端创业人才”项目资助

Effect of Rhizobium Seed Dressing on Growth of Two Alfalfa and Rhizosphere Microbial Diversity

ZHAO Yang-an1, LU Guang-xin1, DENG Ye2, WANG Ying-cheng1, MA Kun1, YAN Hui-lin1, ZHANG Hai-juan1, ZHOU Xue-li3, DOU Sheng-yun3   

  1. 1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
    2. Research Center for Ecological Environment, Chinese Academy of Sciences;Beijing 100080, China;
    3. Qinghai Province Grassland Improvement Experimental Station, Gonghe, Qinghai Province 813000, China
  • Received:2021-07-25 Revised:2021-08-30 Published:2022-03-10

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摘要： 为研究根瘤菌(rhizobium)拌种对两种苜蓿(Medicago sativa Linn)生长及根际土壤微生物多样性的影响，本研究采用田间观测和高通量测序的方法，分别对不同拌种剂量处理的两种苜蓿(‘北林201’和‘北林202’)生长状况和根际土壤微生物群落多样性进行分析。结果表明：拌种处理后，两种苜蓿的株高明显增加，且在拌种浓度为50%时效果最佳；拌种处理影响了两种苜蓿土壤细菌多样性和群落组成，其中，拌种处理增加了两种苜蓿根际土壤细菌微生物物种多样性，却减少了丰富度；两种苜蓿的土壤细菌门水平上优势菌群为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)，拌种处理增加了变形菌门(Proteobacteria)和放线菌门(Actinobacteria)的相对丰度；使用非度量多维尺度法分析表明，拌种处理后两种苜蓿土壤微生物群落结构存在一定的差异，差异不显著。该结果可为微生物拌种对草牧业的可持续发展提供科学依据。

关键词: 拌种, 紫花苜蓿, 高通量测序, 细菌多样性

Abstract: In order to study the effects of different seed dressing(rhizobium) dosages on the growth of two alfalfa and rhizosphere soil microbial diversity in Qinghai Lake area,the methods of field observation and high-throughput sequencing were used to analyze the growth status and rhizosphere soil microbial community diversity of the two alfalfa (‘Beilin 201’ and ‘Beilin 202’). The results showed that the plant height of two alfalfa increased significantly after the seed dressing,and the best concentration was 50%. The seed dressing treatment increased the bacterial microbial species diversity of the two alfalfa rhizosphere soils,but reduced the abundance. The dominant bacterial groups at the soil bacterial phyla level of the two alfalfa were Proteobacteria,Actinobacteria,Firmicutes,and Bacteroidetes. Seed dressing and bacterial fertilizer treatment increased the relative abundance of Proteobacteria and Actinobacteria. Use of non-metric multidimensional scaling method (NMDS) analysis showed that there were certain differences in the soil microbial community structure of the two alfalfa soils after seed dressing,and the difference was not significant. This result would provide a scientific basis for the sustainable development of grass husbandry by microbial fertilizer.

Key words: Seed dressing, Alfalfa, High-throughput sequencing, Bacterial diversity

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