胡杨AHL基因家族生物信息学分析及逆境胁迫下的表达特征

doi:10.11733/j.issn.1007-0435.2023.03.014

草地学报 ›› 2023, Vol. 31 ›› Issue (3): 741-750.DOI: 10.11733/j.issn.1007-0435.2023.03.014

胡杨AHL基因家族生物信息学分析及逆境胁迫下的表达特征

刘行行, 种培芳

甘肃农业大学林学院, 甘肃兰州 730070

收稿日期:2022-10-08 修回日期:2022-11-28 出版日期:2023-03-15 发布日期:2023-04-01
通讯作者: 种培芳,E-mail:zhongpf@gsau.edu.cn
作者简介:刘行行(1993-),男,汉族,甘肃天水人,博士研究生,主要从事林木逆境生理生态研究,E-mail:583997406@qq.com
基金资助:
国家自然基金，荒漠植物红砂根际促生细菌筛选及其调控红砂抗逆性的研究（32160407）；甘肃农业大学青年导师扶持基金，红砂响应盐胁迫的生理特性及耐盐分子机理的研究（GAU-QDFC-2021-10）；优秀研究生“创新之星”项目（2021CXZX-397）；对发展中国家常规性科技援助项目（KY202002011）；红砂、白刺抗旱优良种质繁育技术示范与推广，中央财政林业科技推广示范资金（2020ZYTG15）资助

Bioinformatics Analysis of the AHL Gene Family in Populus euphratica and its Expression Characteristics under Stress

LIU Hang-hang, CHONG Pei-fang

College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2022-10-08 Revised:2022-11-28 Online:2023-03-15 Published:2023-04-01

摘要/Abstract

摘要： AT-hook核定位（AT-hook motif nuclear localized，AHL）蛋白作为一种小型DNA结合蛋白，广泛存在于植物中，在植物生长发育、逆境胁迫和激素信号应答等方面发挥着重要的作用。为全面了解胡杨（Populus euphratica） AHL基因家族的结构及功能，利用生物信息学方法及qRT-PCR对胡杨AHL家族成员的理化性质、基因结构、亚细胞定位以及逆境胁迫下的表达特征进行分析。理化性质分析表明，胡杨AHL家族共有19个成员，除PeAHL16外，其余PeAHL蛋白均为碱性的不稳定亲水蛋白。基因结构分析表明，PeAHL成员全部为内含子缺失型基因。系统进化结果显示，胡杨AHL家族成员可根据基因类型分为8个亚群。保守基序分析显示，聚类在同一分支中的PeAHL基因其motif组成及排列顺序基本相同。在顺式作用元件分析中，响应干旱胁迫的元件其种类和数量最多。实时荧光定量分析表明，PeAHLs的表达受低温、高温、NaCl以及PEG的显著诱导，与对照相比，PeAHLs在PEG处理下表达量最高。本研究结果为胡杨AHL基因家族的深入研究提供理论依据，为进一步解析胡杨AHL基因参与非生物逆境胁迫的分子机制提供参考。

关键词: AHL基因家族, 胡杨, 生物信息学, qRT-PCR

Abstract: AT-hook motif nuclear localized (AHL) protein, a small DNA-binding protein, is widely found in plant and plays an important part in plant growth and development, responses to adversity stress and hormone signaling. In order to comprehensively understand the structure and function of the AHL gene family of Populus euphratica, the physicochemical properties, gene structure, subcellular localization and expression characteristics under stress of the AHL family members of P. euphratica were analyzed by bioinformatics methods and qRT-PCR. It was showed by the physicochemical property analysis that there were 19 members of the AHL family, and all PeAHL proteins are basic and unstable hydrophilic proteins except for PeAHL16. It was showed by the gene structure analysis that all PeAHL members were intron deletion type genes. It was showed by the phylogenetic tree analysis that the members of the PeAHL family could be split into 8 subgroups based on gene types. It was showed by the conservative motif analysis that PeAHL genes clustered in the same branch had essentially the same motif composition and arrangement order. In the analysis of cis-acting elements, the most diverse and abundant elements within the PeAHL genes were found out in response to drought stress. It was showed by the quantitative real-time fluorescence analysis that the expression of PeAHLs was significantly induced by low temperature, high temperature, NaCl as well as PEG, and the expression of PeAHLs was highest under PEG treatment compared with the control-check. The results of this study provide a theoretical basis for the in-depth study of the AHL gene family of P. euphratica and offer a reference to a further analysis on the molecular mechanism of the involvement of PeAHL genes in response to abiotic stresses.

Key words: AHL gene family, Populus euphratica, Bioinformatics, qRT-PCR

中图分类号:

Q344+.13

刘行行, 种培芳. 胡杨AHL基因家族生物信息学分析及逆境胁迫下的表达特征[J]. 草地学报, 2023, 31(3): 741-750.

LIU Hang-hang, CHONG Pei-fang. Bioinformatics Analysis of the AHL Gene Family in Populus euphratica and its Expression Characteristics under Stress[J]. Acta Agrestia Sinica, 2023, 31(3): 741-750.

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胡杨AHL基因家族生物信息学分析及逆境胁迫下的表达特征

Bioinformatics Analysis of the AHL Gene Family in Populus euphratica and its Expression Characteristics under Stress

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