草地早熟禾转录因子PpMYB44基因克隆及非生物胁迫响应分析

doi:10.11733/j.issn.1007-0435.2024.10.006

草地学报 ›› 2024, Vol. 32 ›› Issue (10): 3062-3070.DOI: 10.11733/j.issn.1007-0435.2024.10.006

• 研究论文 • 上一篇

草地早熟禾转录因子PpMYB44基因克隆及非生物胁迫响应分析

陈阳^1,2, 黄小芹¹, 尤学¹, 于浩然¹, 刘殿辉¹, 赵清峰¹, 金一锋^1,2

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006

收稿日期:2024-02-16 修回日期:2024-05-30 发布日期:2024-11-04
通讯作者: 金一锋,E-mail:Jinyifeng8368215@163.com
作者简介:陈阳(1986-),女，汉族,黑龙江齐齐哈尔人,副教授,博士研究生,主要从事草坪草逆境研究,E-mail：Chenyang8368215@126.com
基金资助:
国家自然科学基金(31701958);黑龙江省自然科学基金项目(QC2017026);黑龙江省普通高等学校青年创新人才培养计划项目(UNPYSCT-2018102);黑龙江省基本科研业务费项目(145109518);齐齐哈尔大学学位与研究生教育教学改革研究项目(QUZLTSJG2023014)资助

Cloning and Analysis of the Abiotic Stress Response of PpMYB44 Gene in Poa pratensis L.

CHEN Yang^1,2, HUANG Xiao-qin¹, YOU Xue¹, YU Hao-ran¹, LIU Dian-hui¹, ZHAO Qing-feng¹, JIN Yi-feng^1,2

1. College of Life Science and Agro-Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
2. Heilongjiang Province Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province 161006, China

Received:2024-02-16 Revised:2024-05-30 Published:2024-11-04

摘要/Abstract

摘要： MYB转录因子在植物次生代谢调节、激素信号转导和抗逆等生理生化过程中发挥重要作用。为揭示MYB转录因子对草地早熟禾(Poa pratensis L.)逆境胁迫条件下的响应，本研究克隆了PpMYB44基因，并进行了生物信息学分析，应用qRT-PCR技术对该基因在组织中的表达特异性及非生物胁迫处理下的表达进行了检测。结果显示，PpMYB44包含典型结构域PLN03091超家族，与其在黑麦草(Lolium perenne)中的同源基因相似度较高。草地早熟禾PpMYB44基因存在组织特异性，其相对表达量由高到低依次为根＞茎＞叶；PpMYB44显著响应干旱、盐、磷和氮胁迫，干旱、氮胁迫抑制PpMYB44基因表达；盐和磷显著促进其表达；经植物激素SA、GA处理后， PpMYB44相对表达量下调，而IAA及GABA促进该基因表达。本研究为丰富草地早熟禾转录因子MYB家族在非生物胁迫下的调节机制提供了理论基础。

关键词: 草地早熟禾, 转录因子, PpMYB44, 非生物胁迫, 表达分析

Abstract: MYB transcription factors play a crucial role in various physiological and biochemical processes, including the regulation of plant secondary metabolism,hormone signal transduction,and stress resistance. To investigate the response of MYB transcription factors in kentucky bluegrass (Poa pratensis L.) to stress conditions,we cloned and bioinformatically analyzed the PpMYB44 gene. We also examined the gene’s expression specificity in different tissues and under abiotic stress treatments using qRT-PCR technology. Our results showed that PpMYB44 contains the typical domain PLN03091 superfamily and has a higher degree of similarity to its homologous gene in ryegrass. The expression of the PpMYB44 gene is specific to different tissues,with the highest relative expression in roots,followed by stems and leaves. PpMYB44 significantly responds to drought,salt,phosphorus,and nitrogen stress,with drought and nitrogen stress inhibiting its expression,while salt and phosphorus significantly promoting its expression. Moreover,treatments with the plant hormones SA and GA down-regulated the relative expression of PpMYB44,whereas IAA and GABA promoted its expression. This study provides a theoretical basis for enhancing our understanding of the regulatory mechanism of MYB transcription factors in bluegrass under abiotic stress.

Key words: Kentucky Bluegrass, Transcription factor, PpMYB44, Abiotic stress, Expression analysis

中图分类号:

S688.4

陈阳, 黄小芹, 尤学, 于浩然, 刘殿辉, 赵清峰, 金一锋. 草地早熟禾转录因子PpMYB44基因克隆及非生物胁迫响应分析[J]. 草地学报, 2024, 32(10): 3062-3070.

CHEN Yang, HUANG Xiao-qin, YOU Xue, YU Hao-ran, LIU Dian-hui, ZHAO Qing-feng, JIN Yi-feng. Cloning and Analysis of the Abiotic Stress Response of PpMYB44 Gene in Poa pratensis L.[J]. Acta Agrestia Sinica, 2024, 32(10): 3062-3070.

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草地早熟禾转录因子PpMYB44基因克隆及非生物胁迫响应分析

Cloning and Analysis of the Abiotic Stress Response of PpMYB44 Gene in Poa pratensis L.

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