草地学报 ›› 2026, Vol. 34 ›› Issue (1): 132-141.DOI: 10.11733/j.issn.1007-0435.2026.01.013

• 研究论文 • 上一篇    

三种虉草在低剂量60Co-γ辐射诱变下的生理响应与评价

胡尔艾1,2, 郑凯鑫1,2, 纪佳慧2, 辛冰2, 刘凌云2, 滕珂2, 范希峰2, 岳跃森2, 丁国昌1   

  1. 1. 福建农林大学风景园林与艺术学院, 福建 福州 350002;
    2. 北京市农林科学院草业花卉与景观生态研究所, 农业农村部华北都市农业重点实验室, 北京 100097
  • 收稿日期:2025-08-20 修回日期:2025-10-20 发布日期:2025-12-24
  • 通讯作者: 丁国昌,E-mail:fjdgc@fafu.edu.cn
  • 作者简介:胡尔艾(2001-),女,汉族,贵州安顺人,硕士研究生,主要从事观赏草研究,E-mail:huerai2i@163.com;
  • 基金资助:
    北京市农林科学院科技创新能力建设专项(KJCX20251208,KJCX20230119);云南省科技计划项目(202403AP140045)资助

Physiological Response and Evaluation of Three Phalaris arundinacea Resources to Low-Dose 60Co-γ Radiation-Induced Mutagenesis

HU Er-ai1,2, ZHENG Kai-xin1,2, JI Jia-hui2, XIN Bing2, LIU Ling-yun2, TENG Ke2, FAN Xi-feng2, YUE Yue-sen2, DING Guo-chang1   

  1. 1. College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China;
    2. Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Beijing 100097, China
  • Received:2025-08-20 Revised:2025-10-20 Published:2025-12-24

摘要: 为探究低剂量60Co-γ辐射对虉草资源1、虉草资源2和虉草资源3的生物学效应并区分3种虉草对低剂量辐射的耐受性。采用10 Gy的60Co-γ射线辐射其带芽点根状茎(亲代),研究了该处理对3种虉草子一代(M1代)种子萌发及幼苗生理生化特性的影响。结果表明:辐射显著抑制了3种虉草M1代种子的发芽率、发芽势及发芽指数;生理生化指标显示,虉草资源3的叶绿素含量和可溶性糖含量较对照显著升高,而过氧化物酶活性降低;虉草资源2表现为叶绿素含量、丙二醛含量及过氧化物酶活性下降,可溶性蛋白含量和超氧化物歧化酶活性升高;虉草资源1的可溶性蛋白含量显著降低。相关性分析表明,发芽指标之间、光合色素之间均呈显著正相关,主成分分析结果与相关性分析结果类似。最后通过隶属函数法分析表明,3种虉草的低剂量辐射耐受能力强弱为虉草资源3>虉草资源1>虉草资源2。

关键词: 虉草, 辐射诱变, 种子发芽, 幼苗生理

Abstract: To investigate the biological effects of low-dose 60Co-γ radiation on three Phalaris arundinacea resources and differentiate their radiation tolerance, their rhizomes with bud point (parental generation) were irradiated with 10 Gy of 60Co-γ rays. The effects on seed germination and seedling physiological-biochemical characteristics of the first filial generation (M1) were examined. The results demonstrated that radiation significantly suppressed germination rate, germination potential, and germination index of all three resources. Physiological analysis revealed chlorophyll and soluble sugar content in Phalaris arundinacea 3 significantly increased (P<0.05), but peroxidase (POD) activity decreased. For Phalaris arundinacea 2, chlorophyll, malondialdehyde (MDA) content, and POD activity reduced, and soluble protein and superoxide dismutase (SOD) activity elevated. For Phalaris arundinacea 1, soluble sugar and soluble protein content markedly decreased. Correlation analysis showed significant positive relationships between germination parameters and photosynthetic pigments. Principal component analysis yielded consistent findings with correlation results. The low-dose radiation tolerance hierarchy established by subordinate function analysis was Phalaris arundinacea 3>Phalaris arundinacea 1>Phalaris arundinacea 2.

Key words: Phalaris arundinacea, Radiation mutagenesis, Seed germination, Seedling physiology

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