几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果

doi:10.11733/j.issn.1007-0435.2000.02.005

›› 2000, Vol. 8 ›› Issue (2): 107-114.DOI: 10.11733/j.issn.1007-0435.2000.02.005

几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果

蒋昌顺¹, 邹冬梅¹, Segenet Kelemu²

1. 中国热带农业科学院农牧研究所, 海南省, 儋州市, 571737;
2. Topical Forages Pathology Laboratory, International Centerfor Tropical Agriculture, A.A.6713, Cali, Colombia

收稿日期:1999-12-09 修回日期:2000-04-06 出版日期:2000-05-15 发布日期:2000-05-15
基金资助:
国家外国专家局和国际热带农业中心资助

Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro

Jiang Changshun¹, Zou Dongmei¹, Segenet Kelemu²

1. Tropical Field Crops and Animal Husbandry Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou City, Hainan Province 571737;
2. Topical Forages Pathology Laboratory, International Center for Tropical Agriculture,A. A, 6713,Cali,Colombia

Received:1999-12-09 Revised:2000-04-06 Online:2000-05-15 Published:2000-05-15

摘要/Abstract

摘要： 构建了几丁质酶基因植物表达载体,该基因在大肠杆菌中能表达35kD的蛋白质。几丁质酶粗提物在体外对柱花草胶孢炭疽病原菌有极显著的抑菌效果。

关键词: 几丁质酶, 基因表达, 炭疽病原菌, 抑菌作用

Abstract: Chinase gene expressing vectors for plant transformation were constructed,35kD protein was expressed by chitinase gene in Escherichiacoli DH5α. In vitro crude preparation of chinase has efficient inhibition on the growth of Colletotrichum gloeosporioides, which caused Stylosnathes anthracnose disease.

Key words: Chinase gene, Expressing in Escherichia coli, Inhibition on Colletotrichum gloeosporioides growth

中图分类号:

蒋昌顺, 邹冬梅, Segenet Kelemu. 几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果[J]. , 2000, 8(2): 107-114.

Jiang Changshun, Zou Dongmei, Segenet Kelemu. Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro[J]. , 2000, 8(2): 107-114.

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几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果

Chitinase Gene Expressing in Escherichia coli DH5α and Its Products Inhibiting on the Growth of Colletotrichum gloeosporioides in Vitro

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