草地学报

›› 2013, Vol. 21 ›› Issue (3): 598-606.DOI: 10.11733/j.issn.1007-0435.2013.03.028

• 研究论文 • 上一篇    下一篇

不同居群野生狗牙根材料的SSR分析

张延辉, 帕提古丽, 李江华, 于辉, 阿不来提   

  1. 新疆草地资源与生态重点实验室 新疆农业大学草业与环境科学学院, 新疆 乌鲁木齐 830052
  • 收稿日期:2012-12-24 修回日期:2013-03-20 出版日期:2013-06-15 发布日期:2013-06-05
  • 通讯作者: 阿不来提
  • 作者简介:张延辉(1980- ),男,新疆乌苏人,讲师,博士研究生,主要从事草坪相关的研究,E-mail:zyh1980@sina.cn
  • 基金资助:
    国家自然基金项目(31160483);新疆科技厅高技术项目(20111112);新疆农业大学国家重点学科项目(XJCYB-2011-05);新疆农业大学校前期课题(XJAU201028)资助

SSR Analyses of Different Bermudagrass Populations

ZHANG Yan-hui, Paiti-guli, LI Jiang-hua, YU Hui, Abu-laiti   

  1. Xiniang Key Laboratory of Grassland Resources and Ecology, Department of Grassland and Environment Science of Xinjiang Agriculture University, Urumqi, Xinjiang 830052, China
  • Received:2012-12-24 Revised:2013-03-20 Online:2013-06-15 Published:2013-06-05

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摘要： 为评价新疆狗牙根(Cynodon dactylon)资源的遗传多样性水平,以采自新疆不同地域的狗牙根与部分华南地区及国外的狗牙根为材料,用SSR标记开展遗传多样性研究。结果表明:20对引物共产生142个等位变异,平均每个位点7个,其中有124个等位变异显示多态性,多态位点百分率为87.86%,位点Nei's基因多样性(H)指数平均为0.4347;根据遗传一致性及遗传分化结果,106份狗牙根材料的遗传相似性系数值在0.43~1.0之间,平均0.715,居群间遗传分化系数为0.4119,表明有41.19%的变异存在于居群间,58.81%的变异存在于居群内,居群内的遗传分化大于居群间的遗传分化;根据UPGMA 法聚类表明,106份供试材料相似系数为0.39时可聚为3类,材料间的遗传差异与其采集地的关系不很明显,但来自同一地区的材料较多地聚在一起,遗传背景较近,整体来看聚类结果与地理来源有一定的相关性。

关键词: 狗牙根, 遗传多样性, SSR

Abstract: In order to evaluate the diversity level of Xinjiang Bermudagrass, the genetic diversities of Bermudagrass from Xinjiang, Huanan and abroad were investigated using SSR marker. Results indicated that 142 allelic variations were amplified by 20 primers, of which 124 were polymorphism and each locus had 7 allelic variations. The percentage of polymorphic loci was 87.86%, the average of Nei's (H) was 0.4347. The genetic similarities (GS) of 106 materials ranged from 0.43 to 1.0, the average was 0.715. The genetic differentiation coefficient was 41.19% among the population and 58.81% inside the population. Data illustrated genetic variation within populations was larger than among populations according to genetic identity and differentiation. The general clustering consequences by UPGMA method indicated that 106 materials were classified into three groups when the similarity coefficient was 0.39. The genetic variance of Bermuda grass was not obviously related to the origin, but the materials from the same region were more classified into the same group. These clustering results were correlated with geographic origin in some extent.

Key words: Cynodon dactylon, Genetic diversity, SSR

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