草地学报

草地学报 ›› 2016, Vol. 24 ›› Issue (1): 226-230.DOI: 10.11733/j.issn.1007-0435.2016.01.033

• 简报 • 上一篇    下一篇

转DREB1A基因草地早熟禾对水分亏缺及旱后复水的生理响应

李飞飞, 许立新, 韩烈保   

  1. 北京林业大学草坪研究所, 北京 100083
  • 收稿日期:2015-04-01 修回日期:2015-12-30 出版日期:2016-02-15 发布日期:2016-04-26
  • 通讯作者: 韩烈保
  • 作者简介:李飞飞(1984-),男,河北邯郸人,博士,主要从事草坪逆境生理方面的研究,E-mail:feifeili433@126.com
  • 基金资助:

    国家高技术研究发展计划(863计划)课题:花卉、牧草、竹藤功能基因组学研究与应用(2013AA102607);国家自然科学基金面上项目-麦冬ERF转录因子的克隆与功能研究(31172255)资助

Physiological Responses to Water Deficit and Post-drought Recovery of DREB 1 A Transgenic Kentucky Bluegrass plant

LI Fei-fei, XU Li-xin, HAN Lie-bao   

  1. Institute of Turfgrass Science, Beijng Forestry University, Beijing 100083, China
  • Received:2015-04-01 Revised:2015-12-30 Online:2016-02-15 Published:2016-04-26

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摘要：

为探讨转DREB1A基因草地早熟禾(Poa pratensis)对水分亏缺及旱后复水的生理响应,本研究选择了草地早熟禾‘巴润’品种5个转DREB1A基因株系,在光照生长室内培养12周后,进行水分亏缺及旱后复水处理,测定了草坪质量、叶片相对含水量(RWC)、叶绿素、电解质渗漏(EL)、丙二醛(MDA)、过氧化氢(H2O2)、抗氧化相关酶活性和总非结构性碳水化合物(TNC)。结果表明,转基因植株适应干旱及旱后复水的能力与抗氧化相关酶活性的增加和TNC的积累有关。

关键词: DREB1A, 草地早熟禾, 水分亏缺, 抗氧化代谢, TNC

Abstract:

To explore physiological response to water deficit and post-drought recovery in DREB1A transgenic plants, five transgenic lines of kentucky bluegrass (Poa pratensis) ‘Baron’ cultivar were grown in a growth chamber for 12 weeks, and then were subjected to water deficit and post-drought recovery. Turf quality, leaf relative water content (RWC), chlorophyll, electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2), antioxidant enzyme activities and total nonstructural carbohydrates (TNC) were measured. Results showed the better adaption to water deficit and post-drought recovery of transgenic plants were related to increased antioxidant enzyme (SOD, CAT, POD, APX) activities and accumulation of total nonstructural carbohydrates (TNC).

Key words: DREB1A, Kentucky bluegrass, Water deficit, Antioxidative response, TNC

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