草地学报

草地学报 ›› 2024, Vol. 32 ›› Issue (2): 489-494.DOI: 10.11733/j.issn.1007-0435.2024.02.016

• 研究论文 • 上一篇    下一篇

10份不同种源野生花苜蓿材料种子萌发期抗旱性评价

侯瑞虹1, 杜柯1, 黄伟业1, 高佳荷1, 刘亚玲2, 石凤翎1, 张志强1, 唐芳1   

  1. 1. 内蒙古农业大学草原与资源环境学院, 草地资源教育部重点实验室, 农业部饲草栽培、加工与高效利用重点实验室, 内蒙古 呼和浩特 010010;
    2. 国家草业技术创新中心(筹), 内蒙古 呼和浩特 010070
  • 收稿日期:2023-11-12 修回日期:2023-12-15 出版日期:2024-02-15 发布日期:2024-03-06
  • 通讯作者: 唐芳,E-mail:fta223@imau.edu.cn
  • 作者简介:侯瑞虹(1998-),女,汉族,内蒙古鄂尔多斯人,硕士研究生,主要从事牧草种质资源与育种研究,E-mail:houruihong@emails.imau.edu.cn
  • 基金资助:
    内蒙古自治区科技重大专项（2021ZD0031）；内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目（2022JBGS0040）；2023年国家草业技术创新中心（筹）重大创新平台建设专项（CCPTZX2023K01）；呼和浩特市科技计划项目（2022-社-重-1-2-2）资助

Evaluation on Drought Resistance of 10 Wild Medicago ruthenica L. Materials from Different Provenances at Germination Period

HOU Rui-hong1, DU Ke1, HUANG Wei-ye1, GAO Jia-he1, LIU Ya-ling2, SHI Feng-ling1, ZHANG Zhi-qiang1, TANG Fang1   

  1. 1. Key Laboratory of Grassland Resources, Ministry of Education, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010010, China;
    2. National Grassland Technology Innovation Center (under preparation), Hohhot, Inner Mongolia 010070, China
  • Received:2023-11-12 Revised:2023-12-15 Online:2024-02-15 Published:2024-03-06

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摘要： 为筛选出抗旱花苜蓿（Medicago ruthenica L.）种质资源，为花苜蓿抗旱机理研究和品种选育提供研究材料和理论参考，本研究利用PEG-6000溶液模拟干旱胁迫，以无菌水为对照，测定发芽率、发芽势、胚根长、胚芽长、鲜重及干重6项指标，同时为消除各材料的背景差异，计算各指标的相对值，并通过模糊数学隶属函数法对10份不同种源花苜蓿材料进行抗旱性综合评价。研究表明，在PEG-6000溶液模拟干旱胁迫下，15% PEG-6000溶液可以促进10份花苜蓿材料胚根长的生长，其余指标在干旱胁迫下均受到了一定程度的抑制且随着干旱胁迫程度的增加，抑制作用也随之加大。10份材料中，来自西乌线的7号材料和多伦的2号材料抗旱性最强，来自西霍线的9号和8号材料抗旱性最弱。

关键词: 花苜蓿, 种子萌发期, 抗旱性, 综合评价

Abstract: To screen out the drought-resistant Medicago ruthenica L. germplasm resources,the current study evaluated the drought resistance of 10 wild Medicago ruthenica L. materials from different provenances. This could provide research materials and a theoretical foundation for studying of drought-resistant mechanism and breeding varieties of M. ruthenica. Six indexes including germination rate,germination potential,embryo root length,fresh weight,and dry weight of M. ruthenica seedlings were examined under drought stress simulated by PEG-6000 with sterile water as control. To eliminate the background differences among test materials,the relative values of each index were calculated,and the fuzzy mathematical membership function method was used to comprehensively evaluate the drought resistance of 10 M. ruthenica materials. The results showed that 15% PEG-6000 could promote the root growth of 10 M. ruthenica materials,while the other indexes were somewhat inhibited under the simulated drought stress. As the increase of drought stress extent,the inhibititory effect also intensified. Among 10 wild M. ruthenica materials,Material No.7 from Xiwu Line and Material No.2 from Duolun exhibited the strongest drought resistance,and Material No.9 and Material No.8 from Xihuo Line demonstrated the weakest drought resistance.

Key words: Medicago ruthenica L., Germination stage, Drought resistance, Comprehensive evaluation

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