草地学报 ›› 2024, Vol. 32 ›› Issue (4): 1044-1054.DOI: 10.11733/j.issn.1007-0435.2024.04.007

• 研究论文 • 上一篇    

混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响

朱晨晨1, 史昆1, 何沁坤1, 郑雨琴1, 苑峰2, 刘亚玲2, 王赞1   

  1. 1. 中国农业大学草业科学与技术学院, 北京 100193;
    2. 内蒙古草业技术创新中心有限公司, 内蒙古 呼和浩特 010070
  • 收稿日期:2024-01-12 修回日期:2024-03-20 发布日期:2024-05-07
  • 通讯作者: 王赞,E-mail:zanwang@cau.edu.cn
  • 作者简介:朱晨晨(1996-),女,汉族,山东聊城人,博士研究生,主要从事牧草基因资源挖掘与利用的相关研究,E-mail:zhucc@cau.edu.cn
  • 基金资助:
    国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023B04);国家林业和草原种质资源库(2005DKA21003)资助

Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings

ZHU Chen-chen1, SHI Kun1, HE Qin-kun1, ZHENG Yu-qin1, YUAN Feng2, LIU Ya-ling2, WANG Zan1   

  1. 1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
    2. Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, Inner Mongolia 010070, China
  • Received:2024-01-12 Revised:2024-03-20 Published:2024-05-07

摘要: 本研究以'ZN-1’和'保定’苜蓿(Medicago sativa L.'Baoding’)为试验材料,以浓度梯度为0 mmol·L-1,40 mmol·L-1和80 mmol·L-1,pH值为9.3的混合盐碱溶液(NaCl∶Na2SO4∶NaHCO3∶Na2CO3摩尔比为1∶1∶1∶1)进行胁迫处理,比较2个紫花苜蓿幼苗的生长指标、生理指标和基因表达(ROS清除、离子转运和光合调控3类基因)差异,以期为解析紫花苜蓿耐混合盐碱胁迫响应机制提供理论依据。结果表明,随着混合盐碱胁迫浓度的增加,2个紫花苜蓿的株高、干重、叶绿素含量、光合速率以及Fv/Fm等均出现明显的损伤(P<0.05)。其中,'ZN-1’的耐混合盐碱性显著高于'保定’苜蓿,主要表现为'ZN-1’的株高和生物量更高、叶绿素含量和抗氧化酶的活性更高,净光合速率更强,脯氨酸和可溶性糖含量更高,叶片ROS积累量和地下部Na+积累更少,ROS清除、脯氨酸生物合成、Na+液泡区隔化以及叶绿素维持相关编码基因的表达水平更高。因此,脯氨酸生物合成、Na+液泡区隔化、叶绿素维持以及抗氧化酶可能在'ZN-1’耐混合盐碱胁迫中发挥重要作用。

关键词: 紫花苜蓿, 混合盐碱胁迫, 生理响应, 基因表达

Abstract: In this study,two alfalfa (Medicago sativa L.) materials 'Baoding’ and 'ZN-1’ were treated with the mixed saline-alkali solution (NaCl∶Na2SO4∶NaHCO3∶Na2CO3 in 1∶1∶1∶1 ratio at pH 9.3) at the concentrations of 0 mmol·L-1,40 mmol·L-1 and 80 mmol·L-1,and compared for the tolerance. The morphology,physiology,and expression of the selected genes that are involved in ROS-scavenging,ion transport,and photosynthetic regulation were investigated at the alfalfa seedling stage,to look into the response to mixed saline-alkali stress. The results showed that with the increase of mixed saline-alkali concentration,significant (P<0.05) damage was observed in the plant height,dry weight,chlorophyll content,photosynthetic rate,and Fv/Fm,in both the alfalfa materials. Compared with 'Baoding’,'ZN-1’ demonstrated significantly higher saline-alkali tolerance,as evidenced by higher plant height,more biomass,more contents of chlorophyll content,proline,and soluble sugar,stronger antioxidant activity,promoted robust photosynthesis,and less accumulation of ROS in leaves and Na+in root. In 'ZN-1’,the significantly up-regulated genes were associated with the pathways of ROS scavenging,proline biosynthesis,Na+ vacuolar compartmentalization,and chlorophyll maintenance. In summary,proline biosynthesis,Na+ vacuolar compartmentalization,chlorophyll maintenance,and antioxidants may play an important role in the 'ZN-1’ tolerance of mixed saline-alkali stress.

Key words: Alfalfa, Mixed saline-alkali stress, Physiological responses, Genes expression

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