草地学报

›› 2009, Vol. 17 ›› Issue (5): 570-574.DOI: 10.11733/j.issn.1007-0435.2009.05.006

• 研究论文 • 上一篇    下一篇

2酸度水平下亚铁离子对苜蓿幼苗生理特性的影响

李剑峰1, 张淑卿1, 师尚礼1, 霍平慧1, 安玮2   

  1. 1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心, 甘肃, 兰州, 730070;
    2. 天水市疾病预防控制中心, 甘肃, 天水, 741000
  • 收稿日期:2009-02-23 修回日期:2009-05-26 出版日期:2009-10-15 发布日期:2009-10-15
  • 通讯作者: 师尚礼,E-mail:shishl@gsau.edu.cn
  • 作者简介:李剑峰(1979- ),男,甘肃天水人,博士研究生,研究方向为牧草种质资源和微生物制剂,E-mail:ljfsmart@qq.com
  • 基金资助:
    国家科技支撑项目(2007BAD52B06)、(2006BAD04A04)、(2006BAD01A19);农业部行业专项(nyhyzx07-022);现代农业产业技术体系建设专项资助

Effect of Fe2+ on Physiological Characteristics of Alfalfa Seedling under Two levels of Acidity

LI Jian-feng1, ZHANG Shu-qing1, SHI Shang-li1, HUO Ping-hui1, An Wei2   

  1. 1. Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S.Center for Grazing land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
    2. Tianshui Center of Disease Control, Tianshui, Gansu Province 741000, China
  • Received:2009-02-23 Revised:2009-05-26 Online:2009-10-15 Published:2009-10-15

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摘要： 为探明酸环境和亚铁离子胁迫对紫花苜蓿(Medicago sativa L.)幼苗各生理特性指标的影响,寻求酸性土壤中适宜紫花苜蓿生长发育的环境亚铁离子浓度区间,于2007年2月以苜蓿品种W525为试验材料,采用砂培的方法研究了酸性(pH=4.5)和弱酸性(pH=6)环境中Fe2+(10 mg/kg、50 mg/kg、100 mg/kg,150 mg/kg和200mg/kg)对苜蓿幼苗生理特性的影响。结果表明:酸铁胁迫共存的环境中,叶绿素含量变化主要由环境酸度引起;根系活力对Fe2+浓度变化更为敏感;而光系统Ⅱ光化学效率(Fv/Fm)和潜在活性(Fv/Fo)、可溶性糖含量和膜透性同时受酸环境和Fe2+浓度的共同影响;酸性环境(pH=4.5)中100 mg/kg的Fe2+含量已达到苜蓿幼苗的耐受极限;弱酸环境(pH=6)下150 mg/kg的Fe2+含量才达到苜蓿幼苗的耐受极限,而50 mg/kg的Fe2+浓度尚能促进的苜蓿生长;10 mg/kg~50 mg/kg的Fe2+含量是苜蓿在两种酸度土壤中共同的最适生长区间,两种酸度环境下该Fe2+浓度范围内的根系活力、光化学效率(Fv/Fm)和光系统Ⅱ潜在活性(Fv/Fo)均显著高于100 mg/kg~200mg/kg的Fe2+处理(P<0.05)。

关键词: 紫花苜蓿, 酸胁迫, 亚铁离子, 生理特性

Abstract: Alfalfa(Medicago sativa L.) is a kind of perennial leguminous forage grass with rapid growth,high yield,rich protein content,strong tolerance ability,and broad adaptation and usually be named the king of forage grasses.However,we have to face the stress factors acting on the yield of alfalfa in acid and iron-rich soil.Traditional researches primarily use the sour soil cultivation on the spot to simulate the real environmental stresses.But it’s unable to concretely investigate the effectiveness of single stress factor and unable to determine the dominate stress factor and to propose the corresponding countermeasure under different circumstances.In order to determine the most suitable range of Fe2+ concentration for alfalfa growth in acid soils,the effect of Fe2+ and acidity on the physiological characteristics of alfalfa seedlings were studied using sand culture with different Fe2+ concentration(10,50,100,150,and 200 mg·kg-1) under acid environment of pH 4.5 and pH 6.0.The results show that the change of chlorophyll content was mainly induced by acidity and root activity was more sensitive to the Fe2+ content of than acidity.Chlorophyll fluorescence characteristic,soluble sugar content,and membrane permeability were simultaneously influenced by the effect of acidity and Fe2+ content;100 and 150 mg·kg-1 Fe2+ was the highest tolerance level by alfalfa seedlings at pH 4.5 and pH 6.0,respectively.Physiological characteristics of alfalfa seedlings had better performance than other treatments under environment of pH 6.0 and 50 mg·kg-1 Fe2+(P<0.05).The range of Fe2+ concentration from 10 to 50 mg·kg-1 was the most suitable range for alfalfa seedling at both pH 4.5 and 6.0 and the root activity,PSII activity(Fv/Fo),and PSII photochemical efficiency(Fv/Fm) of alfalfa seedling were higher than those under the Fe2+ concentration from 100 to 200 mg·kg-1(P<0.05).

Key words: Medicago sativa L., Acid stress, Fe2+, Physiological characteristics

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