草地学报

›› 2006, Vol. 14 ›› Issue (3): 214-218.DOI: 10.11733/j.issn.1007-0435.2006.03.004

• 研究报告 • 上一篇    下一篇

胡枝子属植物野生居群遗传多样性RAPD分析

张吉宇1, 袁庆华2, 王彦荣3, 张文淑2   

  1. 1. 兰州大学草地农业科技学院, 甘肃草原生态研究所, 兰州, 730020;
    2. 中国农业科学院畜牧研究所, 北京, 100094
  • 收稿日期:2006-01-16 修回日期:2006-07-05 出版日期:2006-08-15 发布日期:2006-08-15
  • 通讯作者: 袁庆华, E-mail:yuanqinghua@hotmail.com
  • 作者简介:张吉宇(1978- ),男,甘肃民乐人,讲师,从事牧草育种与生物技术的教学和研究,E-mail:zjyfrank@hotmail.com;
  • 基金资助:
    国家“十五”科技部基础性工作专项资助项目(2003-2005年)

RAPD Analysis on Genetic Diversity of Wild Population of Lespedeza Michx.

ZHANG Ji-yu1, YUAN Qing-hua2, WANG Yan-rong3, ZHANG Wen-shu2   

  1. 1. College of Pastoral Agriculture Science and Technology, Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou, Gansu Province, 730020, China;
    2. Institute of Animal Science, CAAS, Beijing, 100094, China
  • Received:2006-01-16 Revised:2006-07-05 Online:2006-08-15 Published:2006-08-15

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摘要： 利用RAPD标记分析胡枝子属14个野生居群遗传多样性,对其60个10碱基随机引物进行筛选,选择能产生多态性谱带的20个引物,共获得209个RAPD标记,182个显多态性,占总谱带数的87.08%。居群间观测等位基因数Aa和有效等位基因数Ae分别为1.8708和1.5868,Nei's基因多样性指数H和Shannon's信息指数I分别为0.3344和0.4921,揭示了居群间存在较高的遗传多样性。遗传距离介于0.1006-0.651之间,14个材料的聚类结果与形态学分类结果有相同的趋势。本研究对胡枝子属植物种质资源的保存和育种利用有一定参考价值。

关键词: 胡枝子属, 野生居群, 遗传多样性, RAPD

Abstract: The genetic diversity within 14 wild populations of Lespedeza Michx.was analyzed by random amplified polymorphic DNA(RAPD) technique.Twenty primers were screened to be polymorphic from 60 10-oligonucleotieds primers.Among the total of 209 RAPD loci,182 loci(87.08%) were detected to be polymorphic.The results indicate that the observed number of alleles(Aa),effective number of alleles(Ae),Nei's gene diversity(H),and Shannon's Information index (I) were 1.8708,1.5868,0.3344,and 0.4921,respectively.The results also show that high genetic diversity was screened among 14 wild Lespedeza populations,the genetic distance among which was varied from 0.1006-0.651.The dendrograms constructed based on Nei's genetic distance of RAPD data showed some similarity with that of the morphology.This study will provide a reference to the preservation of germplasm resources and breeding for Lespedeza Michx.

Key words: Lespedeza Michx., Wild population, Genetic diversity, RAPD analysis

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