Abstract: Overcoming physilogical deterioration of a stored seed sample is one of the most important and difficult tasks in the preservation process.Cryopreservation technique (LN₂, at -196℃)provides a po tential preservation by reducing metabolism to a low level at that all biochemical process are significantly reduced and biological decterioration virtually stopped.Thus,the seed storage life was greatly extended.Nine kind forage seed samples were stored in LN₂ for one year.Then viability,conductivity,respiration and the content of ATP of the seed were measured to determine its vigor.Grass family, legume family and goose foot family were the type of LN₂-tolerant.Especially, Ceratoid et arborescens (Losinsk) Tsien et C.C.Ma and Kochia rpstrata(L.)Schrad,were significant suitable for cryopreservation.

Key words: Cryopreservation technique, Storage life, Seed vigor

摘要： 在牧草种子的贮存过程中,最重要且最难于解决的问题就是克服所贮存种子的生理衰变。超低温技术(LN₂,于－196℃条件)可以将种子内部新陈代谢降低到一个很低的水平,使所有的生化过程处于“停顿”状态,这样便可以大大延长种子的贮存寿命。本实验将9种在常规条件下难于保持活力的牧草种子放入液氮中贮存一年,解冻后测定贮存种子的发芽率、发芽指数、电导率、呼吸强度及ATP含量情况。结果表明,禾本科、豆科、藜科牧草种子属于液氮耐受类型,特别是短命种子驼绒藜与木地肤对液氮贮存表现特别适应。

关键词: 超低温技术, 贮存寿命, 种子活力