Identification and Analysis of BAG Gene Family Members in Alfalfa

doi:10.11733/j.issn.1007-0435.2026.10.006

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (10): 3579-3588.DOI: 10.11733/j.issn.1007-0435.2026.10.006

Identification and Analysis of BAG Gene Family Members in Alfalfa

ZHU Long-kao¹, LI Xiao-hong¹, XIA Jing-xin², YANG Hui-zhen¹, ZENG Xin¹, ZHANG Rui-li¹, ZHANG Li-jing¹, FANG Long-fa¹

1. Lanzhou University State Key Laboratory of Herbage Improvement and Grassland Agroecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730000, China;
2. Dezhou University, Dezhou, Shandong Province 253000, China

Received:2026-01-08 Revised:2026-01-25 Published:2026-10-10

紫花苜蓿BAG基因家族成员鉴定及分析

朱龙考¹, 李小红¹, 夏景欣², 杨惠祯¹, 曾心¹, 张瑞丽¹, 张丽静¹, 方龙发¹

1. 兰州大学草地农业生态系统国家重点实验室/兰州大学农业农村部草牧业创新重点实验室/兰州大学草地农业科技学院, 甘肃兰州 730000;
2. 德州学院, 山东德州 253000

通讯作者: 方龙发, E-mail: fanglf@lzu.edu.cn;张丽静, E-mail: lijingzhang@lzu.edu.cn
作者简介:朱龙考（1999-），男，汉族，贵州盘州人，硕士研究生，主要从事牧草抗逆育种研究，E-mail：zhulk2024@lzu.edu.cn
基金资助:
国家重点研发计划（2023YFF1001504）；中国国家自然科学基金委员会（32201463）资助

Abstract

Abstract: The Bcl-2-associated athanogene （BAG） protein family is a multifunctional family that plays a critical role in plant growth and stress responses. However， the BAG gene family has not yet been systematically characterized in alfalfa （Medicago sativa）. In this study， we analyzed the physicochemical properties， evolutionary relationships， gene structures， conserved motifs， cis-regulatory elements， chromosomal localization， collinearity， and gene expression patterns of the BAG gene family in alfalfa using bioinformatics methods and transcriptome data. The results revealed that the alfalfa BAG gene family consists of eight members， which are unevenly distributed across five chromosomes. Subcellular localization predictions indicated their presence in the nucleus， chloroplasts， and/or cytoplasm. Phylogenetic analysis classified the alfalfa BAG genes into three subfamilies. Cis-regulatory element analysis showed that the alfalfa BAG gene family contains numerous elements associated with plant growth and development， light responsiveness， hormone responses， and biotic/abiotic stress responses. The MsBAG gene family exhibited significant responses to abiotic stresses such as abscisic acid （ABA）， low temperature， drought， and salinity. This study provides a fundamental theoretical basis for further investigation into the potential roles of the BAG gene family in abiotic stress tolerance in alfalfa.

Key words: Alfalfa, BAG gene family, Genome-wide, Phylogenetic analysis, Gene structure, Abiotic stress

摘要： Bcl-2 associated athanogene （BAG）蛋白家族是一个多功能家族，在植物生长和胁迫反应中起着关键作用。然而BAG基因家族在紫花苜蓿中还未被系统鉴定。本研究利用生物信息学方法和转录组数据对紫花苜蓿BAG基因家族的蛋白理化性质、进化关系、基因结构、保守基序、顺式作用元件、染色体定位、共线性关系及基因表达进行了分析。结果表明，紫花苜蓿BAG基因家族成员有8个，不均匀分布在紫花苜蓿5条染色体上；通过亚细胞定位预测显示，在细胞核、叶绿体、细胞质中均有分布；系统发育树将紫花苜蓿BAG基因家族分为3个亚家族；顺式作用元件分析表明紫花苜蓿BAG基因家族含有许多与植物生长发育、光响应、激素响应、生物胁迫/非生物胁迫响应相关的元件；MsBAG基因家族对脱落酸（Abscisic acid，ABA）、低温、干旱和盐等非生物胁迫均具有显著的响应。此研究为进一步研究紫花苜蓿BAG基因家族在非生物胁迫中的潜在作用提供了基本的理论依据。

关键词: 紫花苜蓿, BAG基因家族, 全基因组, 系统进化, 基因结构, 非生物胁迫

CLC Number:

S541.9

ZHU Long-kao, LI Xiao-hong, XIA Jing-xin, YANG Hui-zhen, ZENG Xin, ZHANG Rui-li, ZHANG Li-jing, FANG Long-fa. Identification and Analysis of BAG Gene Family Members in Alfalfa[J]. Acta Agrestia Sinica, 2026, 34(10): 3579-3588.

朱龙考, 李小红, 夏景欣, 杨惠祯, 曾心, 张瑞丽, 张丽静, 方龙发. 紫花苜蓿BAG基因家族成员鉴定及分析[J]. 草地学报, 2026, 34(10): 3579-3588.

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Identification and Analysis of BAG Gene Family Members in Alfalfa

紫花苜蓿BAG基因家族成员鉴定及分析

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