Embryogenic Callus Production and Plant Regeneration of Lolium Perenne Seed Explants for Genetic Manipulation

doi:10.11733/j.issn.1007-0435.2004.04.008

›› 2004, Vol. 12 ›› Issue (4): 289-293.DOI: 10.11733/j.issn.1007-0435.2004.04.008

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Embryogenic Callus Production and Plant Regeneration of Lolium Perenne Seed Explants for Genetic Manipulation

ZHANG Zhen-xia^1,2, CHU Cheng-cai³, XI Jia-bin¹, Chen Ping⁴

1. School of Life Science, Sun Yat-sen University, Guangzhou, Guangdong Province 510275, China;
2. Department of Biology, HanShan Normal College, Chaozhou, Guangdong Province, 521041, China;
3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
4. Department of Agronomy, Zhongkai Agrotechnical College, Guangzhou, Guangdong Province, 510225, China

Received:2003-10-30 Revised:2004-06-22 Online:2004-11-15 Published:2004-11-15

多年生黑麦草种子愈伤组织诱导和植株再生

张振霞^1,2, 储成才³, 席嘉宾¹, 陈平⁴

1. 中山大学生命科学院, 广州, 510275;
2. 韩山师范学院生物系, 广东, 潮州, 521041;
3. 中国科学院遗传与发育生物学研究所, 北京, 100101;
4. 仲恺农业技术学院农学院草学专业, 广州, 510225

通讯作者: 韩兴国
作者简介:张振霞(1975- ),女,甘肃兰州人,博士后,专业方向为牧草基因工程,Email:zzx8411@163.com
基金资助:
国家转基因植物研究与产业化专项(JY03-A-10-01)资助

Abstract

Abstract: The study is focused on the initiation and regeneration of Lolium perenne embroygenic callus. The results show that MS medium is the choice basic medium for initiation and subculture of the callus amongst all the media tested. 5 mg/L 2,4-D is the preferable concentration for plants hormone in induction culture and subculture of the callus. Phytagel is a better coagulant than the home-made agar. 50～75 mg/L selective scope of screening agent G418 is feasible.

Key words: Biotechnology, Lolium perenne L, Tissue culture, Callus induction, Plant regeneration

摘要： 研究并建立了多年生黑麦草胚性愈伤组织诱导及其再生体系.结果表明:不同品种的愈伤组织诱导率不同,最适培养基是MS,愈伤组织诱导的最适2,4-D浓度是5mg/L;继代培养时间对愈伤组织的分化率有很大影响;植物凝胶与国产琼脂相比固化效果更好;筛选剂G418对抗性愈伤的浓度在50mg/L和7mg/L时的筛选效果分别达10.31%和6.%.

关键词: 生物技术, 多年生黑麦草, 组织培养, 愈伤诱导, 植株再生

CLC Number:

Q943.1

ZHANG Zhen-xia, CHU Cheng-cai, XI Jia-bin, Chen Ping. Embryogenic Callus Production and Plant Regeneration of Lolium Perenne Seed Explants for Genetic Manipulation[J]. , 2004, 12(4): 289-293.

张振霞, 储成才, 席嘉宾, 陈平. 多年生黑麦草种子愈伤组织诱导和植株再生[J]. , 2004, 12(4): 289-293.

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Embryogenic Callus Production and Plant Regeneration of Lolium Perenne Seed Explants for Genetic Manipulation

多年生黑麦草种子愈伤组织诱导和植株再生

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