Acta Agrestia Sinica

›› 2006, Vol. 14 ›› Issue (3): 219-222.DOI: 10.11733/j.issn.1007-0435.2006.03.005

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Optimization on RAPD Analytical System of White Clover

ZHANG Xian1, ZHANG Ying-jun1, WU Wei-qun2   

  1. 1. Institute of Grassland Science, China Agricultural University, Beijing, 100094, China;
    2. Yunnan Grassland and Feed Management Section, Kun Ming, 650225, China
  • Received:2006-03-07 Revised:2006-07-04 Online:2006-08-15 Published:2006-08-15

白三叶RAPD分析条件优化

张贤1, 张英俊1, 吴维群2   

  1. 1. 中国农业大学草地研究所, 北京, 100094;
    2. 云南省草山饲料站, 昆明, 650225
  • 通讯作者: 张英俊, E-mail:zhangyj@cau.edu.cn
  • 作者简介:张贤(1979- ),女,贵州毕节人,博士研究生,主要从事牧草育种及牧草生物技术研究;
  • 基金资助:
    云南省省院省校科技合作项目(2003BFEOOA002)”资助

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Abstract: The gradient tests on the concentration of Mg2+, dNTP,random primers,template DNA and Taq DNA polymerase are conducted.In the optimization of RAPD system with a total volume of 25 μL,containing 20ng template DNA,9.9ng random primer,0.4 mmol/L dNTP,3UTaq DNA polymerase,1mmol/L Mg2+,the stabile clear white clover RAPD map was obtained successfully.This Reaction system can be used in analyzing the genetic variation of white clover.

Key words: White clover, RAPD, Condition, Optimization

摘要： 对白三叶RAPD反应体系中的模板DNA用量、随机引物浓度、dNTP浓度、TaqDNA聚合酶浓度、Mg2+浓度分别进行了梯度试验。结果表明,在总体积25μL体系中,模板DNA20ng、引物9.9ng、dNTP浓度0.4mmol/L、T aqDNA聚合酶3U、Mg2+浓度1mmol/L的反应体系可得到稳定清晰的RAPD扩增图谱,该体系可用于白三叶遗传多样性分析。

关键词: 白三叶, RAPD, 条件, 优化

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