Fingerprint Constructing and Genetic Diversity Analyzing of Sorghum Sudanense and Sorghum bicolor × Sorghum sudanense with SSR Markers

doi:10.11733/j.issn.1007-0435.2016.01.021

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (1): 156-164.DOI: 10.11733/j.issn.1007-0435.2016.01.021

Previous Articles Next Articles

Fingerprint Constructing and Genetic Diversity Analyzing of Sorghum Sudanense and Sorghum bicolor × Sorghum sudanense with SSR Markers

WANG Jie¹, GAO Qiu², YANG Guo-feng¹, SUN Juan¹, MA Jin-xing², FENG Bao-chang²

1. Qingdao Agricultural University, Qingdao, Shandong Province 266109, China;
2. National Quality Control ＆ Inspection Centre For Grassland Industry Products, MOA, Beijing 100125, China

Received:2014-12-09 Revised:2015-05-10 Online:2016-02-15 Published:2016-04-26

国审苏丹草和高丹草品种SSR指纹图谱构建及遗传多样性分析

王杰¹, 高秋², 杨国锋¹, 孙娟¹, 马金星², 冯葆昌²

1. 青岛农业大学经济草本植物研究所, 山东青岛 266109;
2. 农业部全国草产品质量监督检验测试中心, 北京 100125

通讯作者: 孙娟
作者简介:王杰(1990-),男,硕士研究生,主要从事草种质资源鉴定研究,E-mail:wj547808556@163.com
基金资助:
国家牧草产业技术体系青岛综合试验站(CARS-35-33)资助

Abstract

Abstract:

Twenty pairs of SSR primers were screened from 108 pairs to explore the DNA fingerprint constructing and genetic diversity analyzing of 10 Sorghum Sudanense varieties and 7 Sorghum bicolor×Sorghum sudanense varieties. The results showed that 20 pairs of primers totally amplified 121 bands, in which 117 bands were polymorphic and the average value of polymorphic information content (PIC) was 0.703. The number of varieties which could be identified using single pair primer ranged from 2 to 14 and the number of primer mix which could be used to identify 17 varieties ranged from 2 to 5. The genetic diversity analyses of 17 varieties showed that the average Nei's genetic diversity index (H) was 0.480, the average information Shannon diversity index (I) was 0.820, and the similarity coefficients of 17 national varieties ranged from 0.58 to 0.96 as well. These results indicated that the national variety of Sorghum Sudanense and Sorghum bicolor×Sorghum sudanense were rich in genetic diversities.

Key words: Sorghum sudanense, Sorghum bicolor×, Sorghum sudanense, Simple sequence repeats, Fingerprinting, Genetic diversity

摘要：

利用SSR分子标记技术,从108对引物中筛选出20对在国审10个苏丹草(Sorghum Sudanense)和7个高丹草(Sorghum bicolor×Sorghum sudanense)品种中进行了指纹图谱构建及遗传多样性分析。结果表明:20对引物在17个品种中共扩增出121条谱带,其中多态性条带有117条,多态性信息含量(PIC)平均为0.703;单对引物能将17个品种区分为2~14个类型,2~5对引物指纹图谱可以将17个品种区分开,可作为鉴别高丹草和苏丹草品种的分子依据;17个品种材料的平均Nei's基因多样性指数(H)为0.480,平均Shannon多样性信息指数(I)为0.820,品种间的相似系数介于0.58~0.96之间,可见,国审苏丹草和高丹草品种具有丰富的遗传多样性。

关键词: 苏丹草, 高丹草, SSR, 指纹图谱, 遗传多样性

CLC Number:

S544.1

WANG Jie, GAO Qiu, YANG Guo-feng, SUN Juan, MA Jin-xing, FENG Bao-chang. Fingerprint Constructing and Genetic Diversity Analyzing of Sorghum Sudanense and Sorghum bicolor × Sorghum sudanense with SSR Markers[J]. Acta Agrestia Sinica, 2016, 24(1): 156-164.

王杰, 高秋, 杨国锋, 孙娟, 马金星, 冯葆昌. 国审苏丹草和高丹草品种SSR指纹图谱构建及遗传多样性分析[J]. 草地学报, 2016, 24(1): 156-164.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2016.01.021

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2016/V24/I1/156

References

[1] 徐玉鹏,武之新,赵忠祥. 苏丹草的适应性及在我国农牧业生产中的发展前景[J]. 草业科学,2003,20(7):23-25
[2] 詹秋文. 高粱-苏丹草杂交种生物能源利用的前景分析[J]. 种子,2009,28(8):81-83
[3] 全国牧草品种审定委员会. 中国牧草登记品种集(修订版) [M]. 北京:中国农业大学出版社,1999:76-84
[4] 全国牧草品种审定委员会. 中国牧草登记品种集[M]. 北京:中国农业出版社,2008:71-82
[5] 农业部牧草与草坪种子质量监督检验测试中心. GB/T2930.1-2930.11-2001,牧草种子检验规程[S]. 北京:中国标准出版社,1982
[6] 石守定. 以草原生态保护补助奖励机制促草种质量监管工作[J]. 中国畜牧兽医报,2013(6):1-2
[7] 黄秀,曾捷,聂刚,等. 牛鞭草品种EST_SSR指纹图谱构建及遗传多样性分析[J]. 热带亚热带植物学报,2014,2(22):165-171
[8] 蒋林峰,张新全,黄琳凯,等. 中国鸭茅主栽品种DNA指纹图谱构建[J]. 植物遗传资源学报,2014,15(3):604-614
[9] 武海,蹇黎,朱利泉. 中国兰花资源分子标记鉴定研究进展[J]. 生物技术通报,2010(9):56-60
[10] 徐艳芳,向珣朝,张艳丽,等. SSR技术对杂交稻种冈优188的纯度鉴定[J]. 种子,2012,31(7):126-128
[11] 郭民华,王志红,黎东亮,等. 利用SSR标记快速检测玉米品种纯度[J]. 农学学报,2013,3(05):4-7
[12] 匡猛,杨伟华,许红霞,等. 中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析[J]. 中国农业科学,2011,44(1):20-27
[13] 张玉翠. 我国棉花品种SSR指纹数据库的初步构建[D]. 北京:中国农业科学院,2012
[14] 李莉,王俊峰,颜廷进,等. 基于SSR标记的山东省小麦DNA指纹图谱的构建[J]. 植物遗传资源学报,2013,14(3):537-541
[15] 杨丽芳,王芝学,梁海永. 利用SSR 技术对部分核桃品种(系)亲缘关系分析研究[J]. 安徽农业科学,2013,41(1):49-51,54
[16] 范建光,张海英,宫国义,等. 西瓜DUS测试标准品种SSR指纹图谱构建及应用[J]. 植物遗传资源学报,2013,14(5):892-899
[17] 詹秋文,李杰勤,汪保华,等. 42份高粱与苏丹草及其2个杂交种DNA指纹图谱的构建[J]. 草业学报,2008,17(6):85-92
[18] 李杰勤,王丽华,詹秋文,等. 高粱EST_SSR标记的建立及其在苏丹草中的应用初探[J]. 草业科学,2010,27(03):112-117
[19] Doyle J J, Doyle J L. Isolation of plant DNA from fresh tissue[J]. Focus,1990,12(12):13-15
[20] 王静毅,陈业渊,黄秉智,等. 部分香蕉品种SSR指纹图谱的构建[J]. 果树学报,2009,26(5):733-738
[21] 马红勃,许旭明,韦新宇,等. 基于SSR标记的福建省若干水稻品种DNA指纹图谱构建及遗传多样性分析[J]. 福建农业学报,2010,25(1):33-38
[22] 乔岩,王汉宁,张成,等. 玉米胚乳突变基因ae连锁累赘的SSR分析[J]. 草业学报,2011,20(1):140-147
[23] 毛培胜,王颖. 牧草种及品种鉴定技术的发展现状与应用前景[J]. 种子,2004,23(2):41-44
[24] Paterson A H, Tang H, Rokhsar D S. The Sorghum bicolor genome and the diversification of grasses[J]. Nature,2009,457(7229): 551-556
[25] 李晓宇,于肖夏,马艳红,等. 高丹草新品系的SSR分析[J]. 中国草地学报,2011,33(5):62-66
[26] 温莹. 高丹草EST_SSR标记的开发及遗传多样性的研究[D]. 内蒙古:内蒙古农业大学,2011
[27] 卢杰. 高丹草EST_SSR引物设计及遗传图谱的构建[D]. 安徽:安徽农业大学,2009
[28] 路景标. 高丹草SSR引物电子PCR及遗传多态性分析[D]. 安徽:安徽农业大学,2010
[29] 纪明妹. 超低氢氰酸高丹草新品系主要农艺特性及SSR分析[D]. 内蒙古:内蒙古农业大学,2011
[30] 朱永群,彭建华,庞良玉,等. 高丹草卫星搭载材料优异种质筛选及SSR分析[J]. 草地学报,2012,20(6):1150-1155
[31] 张敏. 贵州省部分小麦种质资源 SSR 遗传多样性研究及指纹图谱构建[D]. 贵阳:贵州大学,2007

Fingerprint Constructing and Genetic Diversity Analyzing of Sorghum Sudanense and Sorghum bicolor × Sorghum sudanense with SSR Markers

国审苏丹草和高丹草品种SSR指纹图谱构建及遗传多样性分析

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MA Cong-yu, HAN Chong-yang, MA Sai-nan, LI Xu-xu, CAI Jia-bang, WANG Yang, ZHANG Xin-quan, NIE Gang. The Fingerprints of 13 White Clover (Trifolium repens L.) Cultivars Based on SSR Markers [J]. Acta Agrestia Sinica, 2021, 29(9): 1892-1899.
[2]	LUO Ming-xin, LIU Feng-min, CHEN Ji-yan, CUI Jun-tao, ZHANG Wei-li. Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ [J]. Acta Agrestia Sinica, 2021, 29(9): 1992-2000.
[3]	LIANG Xiao-yu, JI Yang, HU Yuan-bin, YI Jun, BAI Shi-qie, ZHANG Jing, ZHANG Xin-quan. Exploitation and Universal Analysis of EST-SSR Molecular Markers in Cichorium intybus [J]. Acta Agrestia Sinica, 2021, 29(9): 2081-2090.
[4]	LIU Ling-yun, FAN Xi-feng, TENG Ke, YUE Yue-sen, CHANG Zhi-hui, WU Ju-ying. Advances in Development of Genetic Diversity of Carex L. [J]. Acta Agrestia Sinica, 2021, 29(6): 1148-1157.
[5]	CHEN Zhi-xiang, LUO Xiao-yan, LI Shuan-lin, WU Ru-yue, WANG Wen-qiang, DING Xi-peng. Genetic Diversity and Population Structure Analysis of the Pigeonpea (Cajanus cajanL.) Germplasm Resources Based on SSR Markers [J]. Acta Agrestia Sinica, 2021, 29(5): 904-911.
[6]	LIANG Guo-ling, LIU Wen-hui, MA Xiang. Phenotypic Diversity of the Panicle Among 590 Covered Oats Germplasm Resources in Apline of Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2021, 29(3): 495-503.
[7]	ZHANG Qi, WEI Zhen-wu, YAN Tian-fang, GENG Xiao-li. Identification and Evaluation of Genetic Diversity of Agronomic Traits in Oat Germplasm Resources [J]. Acta Agrestia Sinica, 2021, 29(2): 309-316.
[8]	AN Ming-zhu, HAN Bo, JIANG Hua, DUAN Xin-hui, WEN Yi-fu, ZHOU Kai. Research Progress of Dactylis Species Origin and Phylogeny Analysis [J]. Acta Agrestia Sinica, 2021, 29(12): 2637-2644.
[9]	WANG Jian-li, LIU Jie-lin, ZHU Rui-fen, ZHONG Peng, YOU Jia, DI Gui-li, HAN Wei-bo, SHEN Zhong-bao. Genetic Diversity Analysis of Major Agronomic Traits in 28 Amaranthus hypochondriacus Germplasm Resources [J]. Acta Agrestia Sinica, 2020, 28(4): 1050-1059.
[10]	YANG Yan-ting, SHI Feng-ling, XU Bo, ZHANG Yu-tong, YAN Wei. Identification and Genetic Diversity of Alfalfa Varieties [J]. Acta Agrestia Sinica, 2020, 28(4): 1060-1067.
[11]	MA Dong-mei, ZHANG Xi-bin, HUANG Ting, WANG Weng-jing, ZHAO Li-juan, MA Qiao-li. Genetic Diversity Analysis of 10 Salt Tolerant Alfalfa [J]. Acta Agrestia Sinica, 2019, 27(6): 1477-1485.
[12]	LIU Ya-ling, GENG Ya-ping, XIE Xiao-dong, WANG Fang, ZHANG Peng-fei. Genetic Diversity and Genetic Structure Analysis of Astragalus Based on SSR Molecular Marker [J]. Acta Agrestia Sinica, 2019, 27(5): 1154-1162.
[13]	DENG Zhen-Shan, LI Mai-ping, HAO Lei, CHEN Kai-kai, LI Jing, LIU Yu-zhen, ZHANG Bao-bao, QI Xiang-ying. Diversity and Plant Growth Promoting Activities of the Culturable Endophytic Bacteria Associated with Pennisetum sp. [J]. Acta Agrestia Sinica, 2019, 27(5): 1213-1221.
[14]	XU Zhen-peng, WAN Tao, CAI Ping, ZHANG Xiao-ming, YI Wei-dong, WAN Yi-chao. Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors [J]. Acta Agrestia Sinica, 2018, 26(1): 70-76.
[15]	NAN Ming, ZHAO Gui-Qin, CHAI Ji-Kuan. Phenotypic Diversity and Comprehensive Evaluations of Avena sativa L. Germplasm in Semi-arid Area of the Loess Plateau [J]. Acta Agrestia Sinica, 2017, 25(6): 1197-1205.