Chromosome Karyotyping of Bothriochloa ischaemum in Shanxi

doi:10.11733/j.issn.1007-0435.2017.01.006

Acta Agrestia Sinica ›› 2017, Vol. 25 ›› Issue (1): 38-41.DOI: 10.11733/j.issn.1007-0435.2017.01.006

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Chromosome Karyotyping of Bothriochloa ischaemum in Shanxi

LI Lei, DONG Kuan-hu

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2016-01-04 Revised:2016-10-31 Online:2017-02-15 Published:2017-05-06

山西白羊草染色体核型分析

李磊, 董宽虎

山西农业大学动物科技学院, 山西太谷 030801

通讯作者: 董宽虎,E-mail:dongkuanhu@126.com
作者简介:李磊(1990-),女,山西繁峙人,硕士研究生,主要从事牧草种质资源和育种研究,E-mail:18735429427@163.com
基金资助:
山西省科技攻关项目“山西野生白羊草的驯化与选育（20120311011-1）”资助

Abstract

Abstract:

Somatic cell chromosome karyotypes of Bothriochloa ischaemum in Shanxi were studied using root tip squash method to provide cytological basis for the germplasm resources and breeding of B. ischaemum. The chromosomes of mitotic metaphase cells from thirty root tips were counted accurately. Five good split phase were chosen for karyotype analyses. The results showed that the chromosome number is 2n=40, and its karyotype formula is K(2n) = 4x = 40 = 34m + 6sm. There are 17 pairs of m and 3 pairs of sm. The karyotype of B. ischaemum is 1B.The karyotype asymmetry coefficient is 58.32%.

Key words: Bothriochloa ischaemum(L.)Keng, Chromosome, Karyotype analysis

摘要：

选用山西白羊草（Bothriochloa ischaemum）种子为材料，采用根尖压片法进行细胞染色体核型分析，为白羊草的种质资源利用及育种提供细胞学依据。观察统计了30个完整的中期分裂相确定染色体数目，并选其中5个分裂相进行核型分析。结果表明，白羊草染色体数为2n=40，核型公式为K（2n）=4x=40=34m+6sm，其中中部着丝粒染色体（m）为17对，近中部着丝粒染色体（sm）为3对，核型类型为1B。核型不对称系数为58.32％。

关键词: 白羊草, 染色体, 核型分析

CLC Number:

S543.9

LI Lei, DONG Kuan-hu. Chromosome Karyotyping of Bothriochloa ischaemum in Shanxi[J]. Acta Agrestia Sinica, 2017, 25(1): 38-41.

李磊, 董宽虎. 山西白羊草染色体核型分析[J]. 草地学报, 2017, 25(1): 38-41.

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Chromosome Karyotyping of Bothriochloa ischaemum in Shanxi

山西白羊草染色体核型分析

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