Identification and Bioinformatics Analysis of GATA Transcription Factor Family in Lycium ruthenicum

doi:10.11733/j.issn.1007-0435.2025.06.002

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (6): 1724-1735.DOI: 10.11733/j.issn.1007-0435.2025.06.002

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Identification and Bioinformatics Analysis of GATA Transcription Factor Family in Lycium ruthenicum

ZENG Yu¹, ZHU Jian-gang², DU Yu¹, LIU Yun¹, JIA Xi-bei¹, GUO Yong-chun¹, MA Rui¹, MA Yan-jun¹

1. Forest College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Gansu Province Academy of Qilian Water Resource Conservation Forests Research Institute, Zhangye, Gansu Province 734099, China

Received:2024-06-27 Revised:2024-08-20 Online:2025-06-15 Published:2025-06-13

黑果枸杞GATA转录因子家族鉴定与生物信息学分析

曾雨¹, 祝建刚², 杜雨¹, 刘筠¹, 贾西贝¹, 郭咏淳¹, 马瑞¹, 马彦军¹

1. 甘肃农业大学林学院, 甘肃兰州 730070;
2. 甘肃省祁连山水源涵养林研究院, 甘肃张掖 734099

通讯作者: 马彦军,E-mail:mayanjun@gsau.edu.cn
作者简介:曾雨（1998-），女，汉族，四川资阳人，硕士研究生，主要从事林木遗传育种研究，E-mal：1498064186@qq.com
基金资助:
甘肃省高校产业支撑计划（2023CYZC-46）；甘肃省自然科学基金（24JRRG016，20JR5RA578）；国家自然科学基金（31760244，31560215）资助

Abstract

Abstract: To investigate the role of GATA transcription factor family in Lycium ruthenicum under stress conditions， this study conducted transcriptome sequencing and analysis on Lycium ruthenicum under different NaCl salt stress concentrations， screened GATA transcription factor family members， and analyzed their physical and chemical properties， conserved structural domains， phylogenetic trees， and expression patterns. The results showed that Lycium ruthenicum contains 34 GATA members， divided into four subfamilies， with amino acid numbers ranging from 94 to 540 aa， molecular weights ranging from 10 223.79 to 60 326.46 Da， and isoelectric points ranging from 5.64 to 10.22. The analysis of conserved motifs showed that LrGATAs members often contain a C-X₂-C-X_17-20-C-X₂-C zinc finger structural domain， motif 1 is a relatively conserved motif in LrGATAs. The expression patterns of different LrGATAs genes under salt stress were different. The results of qPCR showed that after low salt stress， the expression of LrGATA6， LrGATA13 in roots and LrGATA16， LrGATA23 in leaves was upregulated， while after high salt stress， the expression of LrGATA23 in roots and LrGATA19， LrGATA23 in leaves was upregulated. The results of this study can provide scientific evidence for further research on the functions of GATA family genes in Lycium ruthenicum and their application in salt stress resistance.

Key words: Lycium ruthenicum, NaCl stress, GATA transcription factor, Expression analysis

摘要： 为研究黑果枸杞（Lycium ruthenicum）GATA转录因子家族在盐逆境胁迫下的作用，本文对不同浓度NaCl盐胁迫下的黑果枸杞进行转录组测序和分析，筛选GATA转录因子家族成员，并分析其理化性质、保守结构域、系统发育树及表达模式。结果表明，黑果枸杞中共包含34个GATA成员，分为4个亚族，其氨基酸数目介于94~540 aa之间，分子量在10 223.79~60 326.46 Da之间，等电点介于5.64~10.22之间。保守基序的分析表明，LrGATAs成员多含C-X₂-C-X_17-20-C-X₂-C锌指结构域，motif 1是LrGATAs较为保守的基序。盐胁迫下不同LrGATAs基因的表达模式存在差异。荧光定量PCR结果表明，低盐胁迫后，根中LrGATA6，LrGATA13和叶中LrGATA16，LrGATA23基因表达量上调，高盐胁迫后，根中LrGATA23，叶中LrGATA19和LrGATA23基因表达量上调。本研究结果可为深入研究黑果枸杞GATA家族基因的功能及其在盐胁迫抗性中的应用提供科学依据。

关键词: 黑果枸杞, NaCl胁迫, GATA转录因子, 表达分析

CLC Number:

S722.3+6

ZENG Yu, ZHU Jian-gang, DU Yu, LIU Yun, JIA Xi-bei, GUO Yong-chun, MA Rui, MA Yan-jun. Identification and Bioinformatics Analysis of GATA Transcription Factor Family in Lycium ruthenicum[J]. Acta Agrestia Sinica, 2025, 33(6): 1724-1735.

曾雨, 祝建刚, 杜雨, 刘筠, 贾西贝, 郭咏淳, 马瑞, 马彦军. 黑果枸杞GATA转录因子家族鉴定与生物信息学分析[J]. 草地学报, 2025, 33(6): 1724-1735.

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Identification and Bioinformatics Analysis of GATA Transcription Factor Family in Lycium ruthenicum

黑果枸杞GATA转录因子家族鉴定与生物信息学分析

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