Research on Evaluation of Cold Tolerance of Perennial Forage Pennisetum

doi:10.11733/j.issn.1007-0435.2026.09.009

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (9): 3234-3245.DOI: 10.11733/j.issn.1007-0435.2026.09.009

Research on Evaluation of Cold Tolerance of Perennial Forage Pennisetum

ZHAO Chun-qiao^1,2, FANG Feng-guang^1,2, GUO Qiang¹, LI Cui¹, ZHENG Mei¹, YUE Yue-sen¹, FAN Xi-feng¹

1. Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
2. College of Life Science, Agriculture and Forestry, Qiqihar University,Qiqihar, Heilongjiang Province 161006, China

Received:2026-03-03 Revised:2025-05-08 Published:2026-09-11

多年生饲用狼尾草耐寒性评价研究

赵春桥^1,2, 房风光^1,2, 郭强¹, 李翠¹, 郑美¹, 岳跃森¹, 范希峰¹

1. 北京市农林科学院草业花卉与景观生态研究所, 北京 100097;
2. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006

通讯作者: 岳跃森, E-mail: yueyuesen@baafs.net.cn;范希峰, E-mail: fanxifeng@baafs.net.cn
作者简介:赵春桥(1986-),男,汉族,山东临清人,博士,副研究员,主要从事草逆境生理生态方向研究,E-mail:zhaochunqiao@baafs.net.cn

Abstract

Abstract: To clarify the key indicators for evaluating the cold tolerance of perennial forage Pennisetum spp.， a total of 42 Pennisetum germplasms were used as experimental materials in this study. Combining indoor low-temperature simulation with field trials， we analyzed the growth and physiological characteristics at the seedling stage and full growth period， overwintering rate， and their correlations of Pennisetum. The results showed that： Low-temperature stress significantly reduced seedling dry matter accumulation by 41.55% and plant height by 11.53%. The malondialdehyde （MDA） content in leaves and tiller nodes increased significantly by 90.43% and 108.48%， respectively. Osmotic regulation substances and antioxidant enzyme activities exhibited distinct responses to low temperature. A total of 9 indicators including soluble sugar and MDA contents in tiller nodes and leaves were screened out by principal component analysis to characterize cold tolerance at the seedling stage. Four accessions including ‘Sumu No.2’ ‘Guimu No.1’， Hybrid Pennisetum No.7 and Sweet Elephant Grass exhibited strong cold tolerance with an above 75% overwintering rate， while WQ Hybrid Pennisetum and Chinese Forage Grass showed weak cold tolerance with a below 50% overwintering rate.The overwintering rate was significantly negatively correlated with MDA content in tiller nodes， and significantly positively correlated with bud soluble sugar content. Soluble sugar and MDA contents were crucial indicators for characterizing the cold tolerance of Pennisetum. This study provides a theoretical foundation for the breeding and innovation of new Pennisetum germplasms with high cold tolerance.

Key words: Pennisetum americanum×Pennisetum purpureum, Pennisetum purpureum, Cold resistance, Physiological responses, Winter survival rate

摘要： 为明确表征多年生饲用狼尾草（Pennisetum spp.）耐寒性的关键指标，本研究以42份狼尾草种质为材料，采用室内低温模拟试验和田间试验相结合的方法，分析了狼尾草苗期和全生育期生长生理特征、越冬率及相关性。低温胁迫显著降低幼苗干物质累积（41.55%）和株高（11.53%），叶片和分蘖节丙二醛含量显著升高90.43%和108.48%，渗透调节物质以及抗氧化酶活性响应显著，通过主成分分析筛选出分蘖节和叶片可溶性糖（SS）、丙二醛（MDA）含量等9个表征苗期抗寒性的指标；‘苏牧2号’‘桂牧1号’、杂交狼尾草7号和甜象草耐寒性较强（越冬率>75%），武强杂交狼尾草和中华粮草耐寒性较弱（越冬率<50%）；越冬率与分蘖节MDA含量显著负相关，与芽SS含量显著正相关。MDA和SS含量是表征狼尾草耐寒性的重要指标。本研究为高抗寒性饲用狼尾草新种源的创制提供了理论参考。

关键词: 杂交狼尾草, 象草, 耐寒性, 生理响应, 越冬率

CLC Number:

S813.9

ZHAO Chun-qiao, FANG Feng-guang, GUO Qiang, LI Cui, ZHENG Mei, YUE Yue-sen, FAN Xi-feng. Research on Evaluation of Cold Tolerance of Perennial Forage Pennisetum[J]. Acta Agrestia Sinica, 2026, 34(9): 3234-3245.

赵春桥, 房风光, 郭强, 李翠, 郑美, 岳跃森, 范希峰. 多年生饲用狼尾草耐寒性评价研究[J]. 草地学报, 2026, 34(9): 3234-3245.

References

[1] YU F W， HUANG X， WANG G L. High-quality development of animal husbandry： Theoretical explanation and implementation path[J]. China Rural Economy，2021（4）：85-99 于法稳，黄鑫，王广梁. 畜牧业高质量发展：理论阐释与实现路径[J]. 中国农村经济，2021（4）：85-99
[2] LI H S， LI M Q， HUANG X H， et al. Soybean supply security in China under the strategic background of an agricultural power： The current basis， potential assessment， and strategy choices[J]. Agricultural Economic Issues，2024，45（7）：48-58 李辉尚，李美琪，黄晓慧，等. 农业强国战略背景下中国大豆供给安全：现实基础，潜力研判与策略选择[J]. 农业经济问题，2024，45（7）：48-58
[3] NAN Z B， WANG Y R， HE J S， et al. Achievements， challenges and prospects of China’s grass seed industry[J]. Acta Prataculturae Sinica，2022，31（6）：1 南志标，王彦荣，贺金生，等. 我国草种业的成就，挑战与展望[J]. 草业学报，2022，31（6）：1
[4] LIU Z P， LIU W X， YANG Q C， et al. Progress and existing problems in pasture breeding in China[J]. China Science Fund，2023，37（4）：528-536 刘志鹏，刘文献，杨青川，等. 我国牧草育种进展及存在问题[J]. 中国科学基金，2023，37（4）：528-536
[5] LIN K J， LIU Z P， LUO D， et al. Current research status， existing problems and development suggestions of forage germplasm resources[J]. Acta Botanica Sinica，2023，58（2）：241-247 林克剑，刘志鹏，罗栋，等. 饲草种质资源研究现状，存在问题与发展建议[J]. 植物学报，2023，58（2）：241-247
[6] WANG J， SHEN W T， ZHANG Y W， et al. Effects of planting density and cutting frequency on yield and quality of hybrid Pennisetum[J]. Acta Agrestia Sinica，2010，18（4）：589-593 王静，沈文彤，张蕴薇，等. 种植密度和刈割频率对杂交狼尾草产量和品质的影响[J]. 草地学报，2010，18（4）：589-593
[7] ZHANG X， GU H R， DING C L， et al. Analysis of relationship between several biological traits and yield of elephant grass[J]. Acta Agrestia Sinica，2009，17（5）：670-674 张霞，顾洪如，丁成龙，等. 象草若干生物学性状与产量的关系分析[J]. 草地学报，2009，17（5）：670-674
[8] HOU X C， TENG K， GUO Q， et al. Research progress on the Pennisetum genus forage grass[J]. Acta Botanica Sinica，2022，57（6）：814-825 侯新村，滕珂，郭强，等. 狼尾草属牧草研究进展[J]. 植物学报，2022，57（6）：814-825
[9] LIU G B， REN F. Comparative experiments on the forage quality and yield of several forage crops[J]. Crops Journal，2005（4）：20-22 刘贵波，仁甫. 几种饲草作物的饲用品质及产草量比较试验[J]. 作物杂志，2005（4）：20-22
[10] LI P， CHEN X， ZHAO C Q， et al. Research progress on the cultivation and comprehensive utilisation of tall grasses[J]. Acta Botanica Sinica，2024，59（5）：847-860 李萍，陈鑫，赵春桥，等. 高大禾草的栽培与综合利用研究进展[J]. 植物学报，2024，59（5）：847-860
[11] LI J P. Evaluation of cold tolerance of 19 resources of the Pennisetum genus[D]. Kunming：Yunnan Agricultural University，2024：57 李建平. 狼尾草属19份资源耐寒性评价[D]. 昆明：云南农业大学，2024：57
[12] WEN H F， FAN X F， ZHU Y， et al. Research progress on hybrid Pennisetum as an energy crop[J]. Chinese Agricultural Bulletin，2017，33（3）：99-104 温海峰，范希峰，朱毅，等. 杂交狼尾草作为能源植物的研究进展[J]. 中国农学通报，2017，33（3）：99-104
[13] ZOU D M， JIANG C S. Current status and prospects of tropical forage breeding in China[J]. Chinese Grassland，2002，24（5）：65-68 邹冬梅，蒋昌顺. 我国热带牧草育种现状与前景[J]. 中国草地，2002，24（5）：65-68
[14] BURTON G W. Registration of ‘merkeron’ napiergrass[J]. Crop Science，1989，29（5）：cropsci1989.0011183X002900050050x
[15] JING H C， ZHANG J Y， JIN J B， et al. Basic biology and biological breeding of forage crops[J]. National Natural Science Foundation of China，2023，37（4）：537-551 景海春，张景昱，金京波，等. 饲草作物基础生物学与生物育种[J]. 中国科学基金，2023，37（4）：537-551
[16] LU J Y， TIAN H， XIONG J B， et al. Comprehensive evaluation of cold tolerance of seedlings of 14 native Pennisetum materials[J]. Acta Prataculturae Sinica，2024，33（8）：1-10 陆姣云，田宏，熊军波，等. 14份乡土狼尾草材料幼苗的耐冷性综合评价[J]. 草业学报，2024，33（8）：1-10
[17] WU G X， TANG X L， YANG D G. Advances in the study of plant physiological responses to low-temperature stress[J]. Crop Journal，2008（3）：17-19 吴广霞，唐献龙，杨德龙，等.植物低温胁迫生理进展[J]. 作物杂志，2008（3）：17-19
[18] QUAN W， XUE W T， ZHAO T Y， et al. Research progress on the response mechanisms of plants to low temperature stress[J]. Journal of China Agricultural University，2023，28（2）：14-22 权威，薛文通，赵天瑶，等. 植物对低温胁迫的响应机制研究进展[J]. 中国农业大学学报，2023，28（2）：14-22
[19] WANG F， WANG Q， ZHAO X Y. Research progress on the phenotypic and physiological response mechanisms of plants under low temperature stress[J]. Molecular Plant Breeding，2019，17（15）：5144-5153 王芳，王淇，赵曦阳. 低温胁迫下植物的表型及生理响应机制研究进展[J]. 分子植物育种，2019，17（15）：5144-5153
[20] LI Y B， YANG S Q， REN G X， et al. Physiological changes and cold resistance comparison of different Poaceae forage under low-temperature treatment[J]. Acta Ecologica Sinica，2009，29（3）：1341-1347 李轶冰，杨顺强，任广鑫，等. 低温处理下不同禾本科牧草的生理变化及其抗寒性比较[J]. 生态学报，2009，29（3）：1341-1347
[21] MAO T T， ZHAO X Q， BAI X D， et al. Effects of low-temperature stress on photosynthetic performance， antioxidant enzyme system and related gene expression in maize seedlings[J]. China Agricultural Science and Technology Bulletin，2025，27（5）：49-58 毛桃桃，赵小强，柏小栋，等. 低温胁迫对玉米幼苗光合性能，抗氧化酶系统及相关基因表达的影响[J]. 中国农业科技导报，2025，27（5）：49-58
[22] JIANG S J， CHANG Y， FAN W Y， et al. Effects of temperature stress training on lipid peroxidation products and antioxidant enzyme activities in tomato seedlings under low temperature stress[J]. Chinese Agricultural Science Bulletin，2007，23（10）：444-448 姜述君，常缨，范文艳，等. 温度逆境锻炼对低温胁迫下番茄幼苗细胞膜脂质过氧化产物及抗氧化酶活性的影响[J]. 中国农学通报，2007，23（10）：444-448
[23] XIANG H T， ZHENG D F， HE N， et al. Research progress on the physiological responses of plants to low-temperature stress and the alleviating effects of exogenous abscisic acid[J]. Acta Prataculturae Sinica，2021，30（1）：1-10 项洪涛，郑殿峰，何宁，等. 植物对低温胁迫的生理响应及外源脱落酸缓解胁迫效应的研究进展[J]. 草业学报，2021，30（1）：1-10
[24] QIN D L， LI Z， WEI J P， et al. Research progress on crop cold resistance and its chemical regulation mechanisms[J]. Crop Journal，2016（4）：26-35 秦东玲，李钊，尉菊萍，等. 作物抗冷性及其化学控制机理研究进展[J]. 作物杂志，2016（4）：26-35
[25] GUAN X J， OUYANG X R. Research progress on low-temperature cold damage in maize[J]. Crop Research，2004（S1）：353-357 关贤交，欧阳西荣. 玉米低温冷害研究进展[J]. 作物研究，2004（S1）：353-357
[26] LIU G B， JIAO D Z， ZHOU C. Research progress on the biological and ecological characteristics of Leymus chinensis[J]. Ecological Science，2024，43（6）：1-10 刘国兵，焦德志，周婵. 羊草生物学和生态学特性研究进展[J]. 生态科学，2024，43（6）：1-10
[27] JI S X， DAI S J， LIU W. Research progress on the mechanisms of plant response to low temperature stress[J]. Life Sciences，2010，22（10）：973-979 计淑霞，戴绍军，刘炜. 植物应答低温胁迫机制的研究进展[J]. 生命科学，2010，22（10）：973-979
[28] YIN Y Q， HU J B，DENG M J. Research progress on the antioxidant system of plant leaves and their response to abiotic stress[J]. Chinese Agricultural Bulletin，2007，23（1）：105-110 尹永强，胡建斌，邓明军. 植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J]. 中国农学通报，2007，23（1）：105-110
[29] SUN M， LUO Z X， PENG M， et al. Effects of low temperature on reactive oxygen metabolism in rice seedling roots and the expression of related genes[J]. Molecular Plant Breeding，2025，23（24）：1-12 孙萌，罗志雄，彭敏，等. 低温对水稻幼苗根系活性氧代谢及其相关基因表达的影响[J]. 分子植物育种，2025，23（24）：1-12
[30] LIN J Y， CHEN Q， ZHANG L， et al. Research progress on melatonin in plant low-temperature stress[J]. Biotechnology Bulletin，2025，41（7）：1-10 林佳怡，陈强，张磊，等. 褪黑素在植物低温胁迫中的研究进展[J]. 生物技术通报，2025，41（7）：1-10
[31] XU Q， YAN Y， WEI Q， et al. Salicylic acid alleviates cold stress in rice via regulating nutrient absorption， osmotic material content， antioxidation system， and expression of cold tolerance genes[J]. Journal of Plant Growth Regulation，2025，44（6）：3260-3272
[32] HU Y B， LIU Y N， ZHANG L， et al. Preliminary evaluation of cold tolerance of eight Pennisetum forage varieties （lines） under natural low-temperature stress[J]. Grass Science，2025（2）：43-49 胡远彬，刘亚男，张靓，等. 自然低温胁迫下8个狼尾草属牧草品种（系）耐寒性初步评价[J]. 草学，2025（2）：43-49
[33] HU Y， SUN M， LUO D， et al. Transcriptome profiling the response of elephant grass （Cenchrus purpureus） root to cold stress[J]. Grass Research，2025，5（1）：1-15
[34] MAO C， ZHANG Y， YANG D， et al. Proteomic analysis reveals lignin and trehalose pathways involved in cold stress response of elephant grass[J]. International Journal of Biological Macromolecules，2025（338）：149707
[35] WU B， LUO D， YUE Y， et al. New insights into the cold tolerance of upland switchgrass by integrating a haplotype-resolved genome and multi-omics analysis[J]. Genome Biology，2025，26（1）：128

Research on Evaluation of Cold Tolerance of Perennial Forage Pennisetum

多年生饲用狼尾草耐寒性评价研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CAO Lei, YANG Zhong-fu, FENG Guang-yan, ZHANG Xin-quan. Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum [J]. Acta Agrestia Sinica, 2026, 34(9): 3172-3184.
[2]	YANG Yu-jiao, HE Chao-xin, ZHOU Jie, LI Lin-ju, ZHANG Xin-quan, PENG Yan. Identification of the ERF Gene Family in Pennisetum Purpureum and Analysis its Response to Low-Temperature Stress [J]. Acta Agrestia Sinica, 2026, 34(9): 3223-3233.
[3]	WANG Xin, XIAO Zhuo-lin, WEI Zun-zheng, TENG Ke, MU Na, ZHOU Le, YUE Yue-sen, FAN Xi-feng. Creation of a Triploid Hybrid of Pennisetum glaucum ‘Ningza No.4’×P. purpureum ‘purple’ and Screening for High-yielding， High-antocyanin Superior Lines [J]. Acta Agrestia Sinica, 2026, 34(8): 2866-2876.
[4]	JIA Ji-yuan, LIN Song, YANG Dan, JI Shi-jun, ZOU Xiao-bin, HU Zhi-yong, JIN Ya-rong, YAN Hai-dong, HUANG Lin-kai. Comparative Study on Production Performance and Regional Overwintering Ability of Elephant Grass Varieties （Lines） [J]. Acta Agrestia Sinica, 2026, 34(8): 2971-2979.
[5]	HE Chao-xin, YU Shuai, ZHANG Xin-quan, PENG Yan. Identification of True Hybrids in the F₁ Generation of Elephant Grass and Comprehensive Evaluation and Screening of High-yield Germplasm [J]. Acta Agrestia Sinica, 2026, 34(8): 2980-2990.
[6]	YU Shuai, ZUO Su-tian, NIE Gang, JIANG Zhe-ji, LING Yao, ZHANG Xin-quan. Comprehensive Evaluation of Yields and Cold Resistance in New Pennisetum purpureum Breeding Materials [J]. Acta Agrestia Sinica, 2026, 34(8): 3004-3014.
[7]	DUAN Wei, MIJIT Munire, YIMIN Jamilaigul, WANG Yu-xiang, LI Qian. Physiological Responses to Low-Temperature Stress and Cold Resistance Evaluation of 20 Medicago falcata Germplasms [J]. Acta Agrestia Sinica, 2026, 34(2): 611-623.
[8]	CHEN Qi, ZHANG Yan, HUO Xiao-wei, YI Feng-yan, SHI Feng-ling, GAO Cui-ping, LI Zhen-yi, ZHANG Zhi-qiang. Effect of MsTIFY10a Gene on Cold Resistance of Transgenic Alfalfa Plants [J]. Acta Agrestia Sinica, 2025, 33(9): 2786-2795.
[9]	FANG Ting, LUO Jia-jia, ZHANG Yu-ge, MAO Yu-ye, GUO Yu-tong, DONG Rong-shu, CAI Ze-ping. Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum [J]. Acta Agrestia Sinica, 2025, 33(7): 2099-2113.
[10]	LIANG Qing-wei, YANG Xiu-fang, NA Ri-su, YU Xue-ran, HAN Xiao-hua. Effects of Last Cutting on Root Neck Antioxidation of Three Fall Dormancy Rating Alfalfa Varieties [J]. Acta Agrestia Sinica, 2025, 33(1): 119-124.
[11]	LIU Ze-yu, YUAN Zi-tong, SUI Ke, ZHANG Yu-xia, LIU Ting-yu, SHAN Xin-he, ZHANG Zhi-yong, WANG Xian-guo. Comprehensive Evaluation of Cold Resistance of Alfalfa Varieties of the “WL” Series Based on Cold Resistance Coefficients [J]. Acta Agrestia Sinica, 2024, 32(8): 2592-2598.
[12]	ZHU Chen-chen, SHI Kun, HE Qin-kun, ZHENG Yu-qin, YUAN Feng, LIU Ya-ling, WANG Zan. Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings [J]. Acta Agrestia Sinica, 2024, 32(4): 1044-1054.
[13]	LIN Ping-dong, GAO Long, CHEN Xin-zhu. Optimization of Anthocyanins Extraction Process of Red Elephant Grass and Study on its Antioxidant Activity [J]. Acta Agrestia Sinica, 2024, 32(12): 3988-3996.
[14]	YAO Jin, HE Yun, LU Jia-ding, ZHANG Xiao-xia, CUI Ya-lei, MA Sen, WANG Zhi-chang, ZHU Xiao-yan, SHI Ying-hua, LI De-feng, SUN Hao. Winter Survival Rate,Tillering Characteristics,and Production Performance of Autumn-Sown Forage Oats in Henan Province [J]. Acta Agrestia Sinica, 2023, 31(2): 528-539.
[15]	YIN Yu-lin, MA Wen-xin, LI Ya-juan, TIAN Xin-hui, DU Wen-hua. Study on the Cold Resistance of New Strains of Timothy Grass from the Central Part of Gansu Province [J]. Acta Agrestia Sinica, 2021, 29(3): 625-630.