Mycosystema

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Biological characteristics and domestication cultivation of Sparassis subalpina

GE Yanhong, HE Jianqing, HAN Zhen, XU Dong, LIU Haixin

Mycosystema, 2024, 43(12): 240092. DOI: 10.13346/j.mycosystema.240092

试验号 Test No.	A碳源 Carbon source	B氮源 Nitrogen source	C酸碱度 pH	D硫酸镁 MgSO₄ (g/L)	生长速率 Growth rate (cm/d)	菌丝长势 Mycelial growth vigor
①	1葡萄糖 Glucose	1 麦芽浸粉Malt dip powder	1 (5.5)	1 (0.05)	0.28±0.00 a	+++
②	1葡萄糖 Glucose	2 蛋白胨 Peptone	2 (6.0)	2 (0.10)	0.22±0.01 ab	++
③	1葡萄糖 Glucose	3 牛肉膏Beef paste	3 (6.5)	3 (0.15)	0.15±0.02 c	++
④	2果糖 Fructose	1 麦芽浸粉Malt dip powder	2 (6.0)	3 (0.15)	0.27±0.01 a	+++
⑤	2果糖 Fructose	2 蛋白胨 Peptone	3 (6.5)	1 (0.05)	0.26±0.03 a	++++
⑥	2果糖 Fructose	3 牛肉膏Beef paste	1 (5.5)	2 (0.10)	0.19±0.04 bc	+++
⑦	3 乳糖 Lactose	1 麦芽浸粉Malt dip powder	3 (6.5)	2 (0.10)	0.28±0.00 a	++
⑧	3 乳糖 Lactose	2 蛋白胨 Peptone	1 (5.5)	3 (0.15)	0.22±0.01 ab	++
⑨	3 乳糖 Lactose	3 牛肉膏Beef paste	2 (6.0)	1 (0.50)	0.14±0.01c	+
T1	0.65	0.83	0.68	0.67
T2	0.72	0.70	0.63	0.69
T3	0.64	0.62	0.69	0.64
X1	0.22	0.28	0.23	0.23
X2	0.24	0.23	0.21	0.23
X3	0.21	0.21	0.23	0.21
R	0.03	0.07	0.02	0.02
因素主次 Factors of priority	A₂	B₁	C₃	D₂
优水平 Excellent level	A₂B₁C₃D₂

Table 11 Analysis of the results of orthogonal experiments of the mycelial growth of Sparassis subalpina

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Table 1 Orthogonal experimental design
Table 2 Recipe of stock culture medium
Table 3 Screening test of stock culture medium for Sparassis subalpina
Fig. 1 Screening test of stock culture medium for Sparassis subalpina. A: Potato pine needle dextrose agar (PPDA) medium; B: Potato pine sawdust dextrose agar (PSDA) medium; C: Potato dextrose agar (PDA) medium; D: PDA comprehensive medium; E: PDA comprehensive medium mixed with VB₁; F: Carrot pine needles dextrose agar (CPDA) medium; G: Carrot pine sawdust dextrose agar (CSDA) medium; H: Carrot dextrose agar (CDA) medium; I: Combined medium.
Table 4 Effects of different carbon sources on mycelial growth of Sparassis subalpina
Fig. 2 Effects of different carbon sources on mycelial growth of Sparassis subalpina.
Table 5 Effects of different nitrogen sources on mycelial growth of Sparassis subalpina
Fig. 3 Effects of different nitrogen sources on mycelial growth of Sparassis subalpina.
Table 6 Effects of different inorganic salts on mycelial growth of Sparassis subalpina
Fig. 4 Growth of mycelia in different inorganic salt substrates of Sparassis subalpina.
Table 7 Effects of different pH on the mycelial growth of Sparassis subalpina
Fig. 5 Mycelial growth at different pH of Sparassis subalpina.
Table 8 Effects of different growth factors on the mycelial growth of Sparassis subalpina
Fig. 6 Mycelial growth under different growth factor substrates of Sparassis subalpina.
Table 9 Effects of different temperatures on the growth of Sparassis subalpina
Fig. 7 Growth of mycelium at different temperatures of Sparassis subalpina.
Table 10 Effects of different C:N ratios on mycelial growth of Sparassis subalpina
Fig. 8 Growth of mycelia on the media with different C/N ratios of Sparassis subalpina.
Fig. 9 The growth of mycelia in orthogonal experiments.
Table 12 The analysis of variance for the orthogonal test
Fig. 10 Enzyme activities of Sparassis subalpina.
Fig. 11 The growth rate of mycelium in different cultivation substrates. 1: Corn flour; 2: Peanut flour; 3: Soybean flour; 4: Highland barley flour; 5: Wheat flour; 6: Glutinous rice flour. The same below.
Table 13 The influence of different formulations on the formation of fruiting bodies
Table 14 The influence of different formulations on the formation of fruiting bodies
Fig. 12 The content of organic matter in different cultivation substrate.
Fig. 13 The whole period of cultivation.
Fig. 14 Cultivated fruiting bodies.

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