草地学报

草地学报 ›› 2023, Vol. 31 ›› Issue (10): 2960-2967.DOI: 10.11733/j.issn.1007-0435.2023.10.007

• 研究论文 • 上一篇    下一篇

施肥对盐碱地饲用燕麦根际土壤微生物多样性的影响

孟利芳1, 姚晓翠1, 但瑶1, 许兴2, 郭庆茹3, 李欣4, 毛桂莲1   

  1. 1. 宁夏大学生命科学学院, 宁夏 银川 750021;
    2. 宁夏大学农学院, 宁夏 银川 750021;
    3. 农业技术推广服务中心, 宁夏 石嘴山 753600;
    4. 农业综合开发中心, 宁夏 石嘴山 753000
  • 收稿日期:2023-03-15 修回日期:2023-04-25 出版日期:2023-10-15 发布日期:2023-11-02
  • 通讯作者: 毛桂莲,E-mail:fransis-0327@163.com
  • 作者简介:孟利芳(1998-),女,汉族,甘肃酒泉人,硕士研究生,主要从事植物生理学研究,E-mail:menglifang911@163.com
  • 基金资助:
    宁夏回族自治区重点研发计划项目（2022BBF0303）；宁夏回族自治区农业育种专项（2019NYY20401）；国家重点研发计划项目（2021YFD1900600）资助

Effects of the Fertilization on the Microbial Diversity in the Rhizospheric Soil of Forage Oats in Salt-alkali Land

MENG Li-fang1, YAO Xiao-cui1, DAN Yao1, XU Xing2, GUO Qing-ru3, LI Xin4, MAO Gui-lian1   

  1. 1. School of Life Sciences, Ningxia University, Yinchuan, Ningxia 750021, China;
    2. School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China;
    3. Agricultural Technology Extension Service Center, Shizuishan, Ningxia 753600, China;
    4. Agricultural Comprehensive Development Center, Shizuishan, Ningxia 753000, China
  • Received:2023-03-15 Revised:2023-04-25 Online:2023-10-15 Published:2023-11-02

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摘要： 为了探究不同施肥模式对盐碱地饲用燕麦（Avena sativa L.）根际土壤微生物多样性的影响，本文设置盐碱地饲用燕麦‘甜燕1号’不施肥、施用常规肥和控释肥模式，基于16S rRNA和内转录间隔区（Internal transcribed spacer，ITS）基因高通量测序技术分别对根际土壤细菌和真菌多样性进行生物信息学分析。结果显示，施肥比不施肥模式增加了根际土壤细菌和真菌的α和β多样性，其中控释肥模式下细菌和真菌群落的操作性分类单元（Operational taxonomic unit，OTU）数量最高。3种施肥模式下优势细菌门均为放线菌门（Actinobacteria）和变形杆菌门（Proteobacteria），优势真菌门均为子囊菌门（Ascomycota）和担子菌门（Basidiomycota）。相关性分析表明，细菌和真菌的优势菌门相对丰度与pH值和全盐含量呈负相关关系，与土壤养分中的全磷含量相关性最高。综上，施肥改变了根际土壤细菌和真菌群落的多样性，且施控释肥比施常规肥提高了饲用燕麦根际土壤微生物多样性。研究结果可为盐碱地饲用燕麦施肥模式的选择提供理论支撑。

关键词: 盐碱地, 饲用燕麦, 施肥模式, 根际土壤, 细菌和真菌多样性

Abstract: To explore the effect of the different fertilization modes on the microbial diversity in the forage oats rhizospheric soil in saline-alkali land,we set up three fertilization modes:no fertilization,conventional fertilization and controlled-release fertilization to forage oats ‘Tianyan No.1’ in saline-alkali soil in this study,and 16S rRNA and ITS high-throughput sequencing were employed to investigate the bacterial and fungal diversity from rhizospheric soil,further be analyzed in the context of bioinformatics. The results showed that fertilization modes increased α and β diversity of the rhizospheric soil bacterial and fungal than no fertilization mode,among which the bacterial and fungal community under controlled-release fertilizer mode had the highest OTU number. The dominant bacterial phyla were mainly Actinobacteria and Proteobacteria,and the dominant fungal phyla mainly Ascomycota and Basidiomycota in three fertilization modes. Correlation analysis showed that the relative abundance of dominant bacterial and fungal phyla was negatively correlated with pH and total salt content,and was most significantly related to total phosphorus content in soil nutrients. These results concluded that fertilizer application changed the diversity of rhizosphere soil bacterial and fungal communities;notably,that controlled-release fertilizer application increased the microbial diversity of rhizospheric soil of forage oats compared with conventional fertilizer application. The results of this research could provide theoretical support for the selection of fertilization mode for forage oats in saline-alkali lands.

Key words: Saline-alkali land, Forage oats, Fertilization mode, Rhizospheric soil, Bacterial and fungal diversity

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