甜高粱苗期镉耐性和富集能力综合评价

doi:10.11733/j.issn.1007-0435.2023.10.016

草地学报 ›› 2023, Vol. 31 ›› Issue (10): 3040-3049.DOI: 10.11733/j.issn.1007-0435.2023.10.016

甜高粱苗期镉耐性和富集能力综合评价

黄娟, 周瑜, 张亚勤, 李泽碧, 王秋月, 奚江, 吴修林

重庆市农业科学院特色作物研究所, 重庆 402160

收稿日期:2023-03-27 修回日期:2023-06-29 出版日期:2023-10-15 发布日期:2023-11-02
通讯作者: 周瑜,E-mail:xinganmermer@163.com
作者简介:黄娟(1989-),女,汉族,重庆巴南人,硕士,助理研究员,主要从事高粱遗传改良与栽培生理研究,E-mail:hj.198800@163.com
基金资助:
重庆市自然科学基金面上项目（cstc2019jcyj-msxmX0125）；重庆市科研机构绩效激励引导专项（cstc2022jxjl80016）；重庆市技术创新与应用发展专项重点项目（cstc2021jscx-gksbX0014）；重庆市财政项目（cqaas2023sjczqn027）资助

Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage

HUANG Juan, ZHOU Yu, ZHANG Ya-qin, LI Ze-bi, WANG Qiu-yue, XI Jiang, WU Xiu-lin

Institute of Characteristic Crops Research, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China

Received:2023-03-27 Revised:2023-06-29 Online:2023-10-15 Published:2023-11-02

摘要/Abstract

摘要： 为筛选和鉴定出镉（Cadmium，Cd）富集能力强的甜高粱（Sorghum bicolor L.）品种，以期为利用甜高粱修复Cd污染土壤提供优异材料。本研究以26个甜高粱品种为材料，采用沙培盆栽试验，设置Cd胁迫（5 mg·kg^-1 Cd²⁺）和对照（0 mg·kg^-1）2个处理，测定苗期甜高粱生长特性和生理指标以及根部、茎叶的Cd含量，以各单项指标的耐性指数作为衡量耐Cd性的依据，运用主成分分析、隶属函数法和系统聚类综合评价Cd耐性和富集能力。结果表明：根长、株高、根鲜重、根干重、茎叶鲜重、茎叶干重、叶绿素含量、POD活性、SOD活性、MDA含量在品种和Cd处理间均存在极显著差异（P<0.01）。主成分分析将10个单项指标转化成3个相互独立的综合指标，利用隶属函数法分析，得到不同品种苗期耐Cd性综合评价值（D值）；通过对D值进行聚类分析，筛选出3份耐Cd性强的品种；进一步采用欧氏距离法对D值、富集系数和转移系数进行聚类分析，将26个甜高粱品种划分成4类，其中‘辽甜6号’和‘F10’属于Cd耐性强且富集转移性强的品种，适用于修复土壤Cd污染。

关键词: 甜高粱, Cd耐性, Cd富集能力, 综合评价

Abstract: The objective of this study was to screen out and identify the varieties of sweet sorghum (Sorghum bicolor L.) with strong concentration of Cd (Cadmium),and to provide excellent materials for the remediation of Cd contaminated soil. A sand culture pot experiment with Cd stress (5 mg·kg^-1 Cd²⁺) and control (0 mg·kg^-1) treatments was conducted to investigate the growth characteristics,physiological indicators,and Cd content in roots and shoots of seedlings of 26 sweet sorghum varieties. The Cd tolerance and enrichment ability of 26 sweet sorghum varieties was evaluated by principal components analysis,membership function analysis and systematic cluster analysis. The results showed that there were significant differences in root length,plant height,root fresh weight,root dry weight,shoots fresh weight,shoots dry weight,chlorophyll content,POD activity,SOD activity and MDA content between varieties and Cd treatment (P<0.01). The 10 individual indicators could be classified into 3 independent comprehensive components by principal components analysis. The cadmium-tolerance comprehensive evaluation value (D value) of different cultivars at seedling stage was obtained by membership function analysis. Three varieties with strong Cd tolerance were selected by cluster analysis of D value. Twenty-six sweet sorghum varieties were divided into 4 categories by euclidean distance method based on the D value,bioconcentration factor coefficient and transfer coefficient. In conclusion ‘Liaotian 6’ and ‘F10’ were suitable for phytoremediation in Cd contaminated soil because of their higher tolerance to Cd and better enrichment ability.

Key words: Sweet sorghum, Cadmium tolerance, Cadmium enrichment ability, Comprehensive evaluation

中图分类号:

S156

黄娟, 周瑜, 张亚勤, 李泽碧, 王秋月, 奚江, 吴修林. 甜高粱苗期镉耐性和富集能力综合评价[J]. 草地学报, 2023, 31(10): 3040-3049.

HUANG Juan, ZHOU Yu, ZHANG Ya-qin, LI Ze-bi, WANG Qiu-yue, XI Jiang, WU Xiu-lin. Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage[J]. Acta Agrestia Sinica, 2023, 31(10): 3040-3049.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I10/3040

参考文献

[1] 宋伟,陈百明,刘琳. 中国耕地土壤重金属污染概况[J]. 水土保持研究,2013,20(2):93-298
[2] VERKLEIJ J,GOLAN-GOLDHIRSH A,ANTOSIEWISZ D M,et al. Dualities in plant tolerance to pollutants and their uptake and translocation to the upper plant parts[J]. Environmental and Experimental Botany,2009,67(1):10-22
[3] LARS J,AKESSON A. Current status of cadmium as an environmental health problem[J]. Toxicology and Applied Pharmacology,2009,238(3):201-208
[4] 张杨杨,李希铭,高鹏,等. 不同浓度镉胁迫下6种草本植物的耐性及富集特征的比较[J]. 草地学报,2021,29(6):1265-1276
[5] 郭智,黄苏珍,原海燕. Cd胁迫对马蔺和鸢尾幼苗生长、Cd积累及微量元素吸收的影响[J].生态环境,2008,17(2):651-656
[6] 徐涛英. 商陆内生菌促进甜高粱生长和重金属土壤修复的研究[D]. 长沙:湖南大学,2011:42
[7] TRA D T T,XIAO H,ZHANG S P,et al. Photosynthetic responses of sweet sorghum cultivars to cadmium toxicity[J]. Journal of Agricultural Science and Technology A,2015,5(12):521-527
[8] DÍAZ-NAVA L E,MONTES-GARCIA N,DOMÍNGUEZ J M,et al. Effect of carbon sources on the growth and ethanol production of native yeast Pichia kudriavzevii ITV-S42 isolated from sweet sorghum juice[J]. Bioprocess and Biosystems Engineering,2017,40(7):1069-1077
[9] STICKLEN M B. Feedstock crop genetic engineering for alcohol fuels[J]. Crop Science,2007,47(6):2238-2248
[10] 王凯荣,龚惠群,王久荣. 栽培植物的耐镉性与镉污染土壤的农业利用[J]. 农业环境保护,2000,19(4):196-199
[11] 杜瑞英,聂呈荣,林初夏,等. 镉污染土壤对潜在能源植物生长的影响[J]. 生态环境,2006,15(4):735-738
[12] METWALI E M R,GOWAYED S M H,AL-MAGHRABI O A,et al. Evaluation of toxic effect of copper and cadmium on growth,physiological traits and protein profile of wheat (Triticum aestivium L.),maize (Zea mays L.) and sorghum (Sorghum bicolor L.)[J]. World Applied Sciences Journal,2013,21(3):301-314
[13] ZANCHETA A C F,ABREU C A D,ZAMBROSI F C B,et al. Cadmium accumulation by jack-bean and sorghum in hydroponic culture[J]. International Journal of Phytoremediation,2015,17(3):298-303
[14] JIA W T,LV S L,FENG J J,et al. Morphophysiological characteristic analysis demonstrated the potential of sweet sorghum (Sorghum bicolor (L.) Moench) in the phytoremediation of cadmium-contaminated soils[J]. Environmental Science and Pollution Research International,2016,23(18):18823-18831
[15] JIA W T,MIAO F F,LV S L,et al. Identification for the capability of Cd-tolerance,accumulation andtranslocation of 96 sorghum genotypes[J]. Ecotoxicology and Environmental Safety,2017,145:391-397
[16] CHEN C,WANG X,WANG J L. Cadmium phytoextraction from loam soil in tropical southern China by Sorghum bicolor[J]. International Journal of Phytoremediation,2017,19(6):572-578
[17] 籍贵苏,永路,吕芃,等. 不同高粱种质对污染土壤中重金属吸收的研究[J]. 中国生态农业学报,2014,22(2):185-192
[18] 再吐尼古丽·库尔班,吐尔逊·吐尔洪,阿不都热依木·卡德尔,等. 甜高粱对土壤重金属Cd的吸收规律[J]. 西北农林科技大学学报,2012,40(12):152-156
[19] 吐尔逊·吐尔洪,再吐尼古丽·库尔班,叶凯. 重金属Cd和Pb在甜高粱幼苗体内的积累特性研究[J]. 中国农学通报,2013,29(3):80-85
[20] 张树攀. 高粱属牧草对土壤重金属镉的响应及富集效应的研究[D]. 扬州:扬州大学,2010:15-52
[21] 黄娟,周瑜,张亚勤,等. 减氮增密对杂交糯高粱籽粒产量和干物质生产的影响[J]. 华北农学报,2022,37(S1):154-160
[22] 杨柳,何正军,赵文吉,等. 狭叶红景天幼苗对水分及遮阴的生长及生理生化响应[J]. 生态学报,2017,37(14):4706-4714
[23] FAN K C,HIS H C,CHEN C W,et al. Cadmium accumulation and tolerance of mahogany (Swietenia macrophylla) seedlings for phytoextraction applications[J]. Journal of Environmental Management,2011,92(10):2818-2822
[24] 胡冰钰,方志刚,娄来清,等. 14份柳枝稷种质资源苗期耐镉性综合评价[J]. 草业学报,2019,28(1):27-36
[25] 张彦,王劲松,董二伟,等. 中晚熟区主要高粱品种耐瘠性综合评价[J]. 中国农业科学,2021,54(23):4954-4968
[26] 田小霞,李丽,毛培春,等. 马蔺苗期耐镉性分析及鉴定指标筛选[J]. 核农学报,2018,32(3):0591-0599
[27] 王慧慧,王晨,张明华,等. 砧用南瓜幼苗对镉的耐性和积累能力研究[J]. 西北植物学报,2018,38(12):2257-2266
[28] 孙建云. 甘蓝(Brassica oleracea L.)耐镉性的品种差异及其机理研究[D]. 南京:南京农业大学,2011:51-52
[29] LU Z W,ZHANG Z,SU Y,et al. Cultivar variation in morphological response of peanut roots to cadmium stress and its relation to cadmium accumulation[J]. Ecotoxicology and Environmental Safety,2013,91:147-155
[30] 张永平,范红伟,杨少军,等. 外源水杨酸对镉胁迫下甜瓜幼苗生长、光合作用和活性氧代谢的缓解效应[J]. 植物生理学报,2014,50(10):1555-1562
[31] 张茹,赵宝平,王永宁,等. 不同镉浓度对3个燕麦品种光合特性及镉富集转运系数的影响[J]. 草地学报,2022,30(8):2089-2099
[32] 薛永,王苑螈,姚泉洪,等. 植物对土壤重金属镉抗性的研究进展[J]. 生态环境学报,2014,23(3):528-534
[33] JINADASA N,COLLINS D,HOLFORD P,et al. Reactions to cadmium stress in a cadmium-tolerant variety of cabbage(Brassica oleracea L.):is cadmium tolerance necessarily desirable in food crops?[J]. Environmental Science and Pollution Research,2015,23(6):5296-5306
[34] 岳松青,曹辉,荀咪,等. 基于指标综合分析的苹果砧木耐镉性评价[J]. 植物生理学报,2019,55(5):649-656
[35] 刘春,杜雪玲,施昌倩,等. 幼苗期生菜耐镉性综合评价与低镉积累种质的筛选[J]. 湖南农业大学学报(自然科学版),2021,47(6):648-654
[36] 张付贵,肖欣,闫贵欣,等. 甘蓝型油菜幼苗期耐镉性评价方法的研究[J]. 中国油料作物学报,2017,39(1):047-054
[37] 余玮,揭雨成,邢虎成,等. 苎麻耐镉品种差异及其筛选指标分析[J]. 作物学报,2011,37(2):348-354
[38] 周瑜,黄娟,吴毓,等. 基于主成分和隶属函数分析的高粱品种耐深播性综合评价[J]. 干旱地区农业研究,2020,38(2):214-220
[39] 张英,马玉申,汪娅梅,等. 苎麻种质资源镉富集能力基因型差异研究[J]. 中国农业科技导报,2021,23(12):54-65
[40] TSUBOI K,SHEHZAD T,YONEDA J,et al. Genetic analysis of cadmium accumulation in shoots of sorghum landraces[J]. Crop Science,2017,57(1):22-31
[41] 孙婕妤,MUHAMMAD L,李强,等. 不同地被菊品种对镉的富集特性及生理响应[J]. 吉林农业大学学报,2018,40(1):92-98
[42] GUO Q,MENG L,MAO P C,et al. An assessment of agropyron cristatum tolerance to Cadmium contaminated soil[J]. Biologia Plantarum,2014,58(1):174-178
[43] 陈建军,于蔚,祖艳群,等. 玉米(Zea mays)对镉积累与转运的品种差异研究[J]. 生态环境学报,2014,23(10):1671-1676
[44] LI Y,LIU K H,WANG Y,et al. Improvement of cadmium phytoremediation by Centella asiatica L. after soil inoculation with cadmium-resistant Enterobacter sp. FM-1[J]. Chemosphere,2018,202:280-288
[45] TAIWO A M,GBADEBO A M,OYEDEPO J A,et al. Bioremediation of industrially contaminated soil using compost and plant technology[J]. Journal of Hazardous Materials,2016,304(5):166-172
[46] YOON J K,CAO X D,ZHOU Q X,et al. Accumulation of Pb,Cu,and Zn in native plants growing on a contaminated Florida site[J]. Science of the Total Environment,2006,368(2-3):456-464
[47] 聂发辉. 关于超富集植物的新理解[J]. 生态环境,2005,14(1):136-138
[48] 王宝媛,濮阳雪华,宋桂龙,等. 20个高羊茅品种镉耐性评价及富集特征[J]. 草地学报,2017,25(1):107-114
[49] 胡旭,郝江珊,王新雨,等. 181份海雀稗种质耐镉性和镉富集能力评价[J]. 分子植物育种,2021,19(18):6191-6200

甜高粱苗期镉耐性和富集能力综合评价

Comprehensive Evaluation of Cadmium Tolerance and Enrichment Ability of Sweet Sorghum at Seedling Stage

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