大狼毒入侵对香格里拉山地草甸土壤微生物特征的影响

doi:10.11733/j.issn.1007-0435.2025.03.011

草地学报 ›› 2025, Vol. 33 ›› Issue (3): 778-788.DOI: 10.11733/j.issn.1007-0435.2025.03.011

• 研究论文 • 上一篇

大狼毒入侵对香格里拉山地草甸土壤微生物特征的影响

杨欣, 肖雪, 牛琼梅, 肖伟, 段蕊, 次旺, 侯晓晗, 初晓辉, 单贵莲

云南农业大学动物科学技术学院, 云南昆明 650201

收稿日期:2024-07-15 修回日期:2024-08-28 发布日期:2025-04-07
通讯作者: 单贵莲,E-mail:shanguilian8203@126.com;初晓辉,E-mail:478491637@qq.com
作者简介:杨欣（1999-）,女,汉族,云南楚雄人,硕士研究生,主要从事草地资源利用与可持续管理研究,E-mail：2522948093@qq.com;肖雪（1988-）,女,汉族,内蒙古赤峰人,博士,主要从事草地生态恢复学研究,E-mail：827145744@qq.com
基金资助:
云南省农业联合专项（202101BD070001-068）;202301BD07001-134,202301BD07001-174;云南省万人计划青年拔尖人才专项（YNWR-QNBJ-2018-031）资助

Effects of Euphorbia jolkinii Invasion on Soil Microbial Characteristics in Shangri La Mountain Meadows

YANG Xin, XIAO Xue, NIU Qiong-mei, XIAO Wei, DUAN Rui, CI Wang, HOU Xiao-han, CHU Xiao-hui, SHAN Gui-lian

College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China

Received:2024-07-15 Revised:2024-08-28 Published:2025-04-07

摘要/Abstract

摘要： 大狼毒（Euphorbia jolkinii）是滇西北山地草甸广泛分布的毒害草,其恣意扩张对当地草地生态系统造成了严重影响。为探明大狼毒扩张对滇西北退化山地草甸土壤微环境的影响,本研究根据大狼毒在退化草地群落中呈斑块状分布的特点,选取大狼毒未入侵斑块、大狼毒低覆盖度斑块（大狼毒覆盖度为8.9%）、大狼毒高覆盖度斑块（大狼毒覆盖度为38.2%）3个区分度明显的斑块类型,取样测定不同斑块土壤样品的养分含量,分析大狼毒扩张对土壤微生物群落组成、结构和多样性的影响。结果表明,大狼毒扩张使土壤养分含量显著增加,大狼毒入侵斑块土壤全氮（Total nitrogen,TN）、土壤有机碳（Soil organic carbon,SOC）、全磷（Total phosphorus,TP）、微生物量碳（Microbial biomass carbon,MBC）、微生物量氮（Microbial biomass nitrogen,MBN）、微生物量磷（Microbial biomass phosphorus,MBP）、速效氮（Available nitrogen,AN）、速效磷（Available nitrogen phosphorus,AP）和速效钾（Available k,AK）含量显著增加（P<0.05）。大狼毒入侵显著改变了土壤细菌群落的组成和结构,显著提高了土壤细菌群落的多样性和真菌群落的丰富度（P<0.05）,土壤细菌和真菌群落对大狼毒蔓延扩张的反应不一致。相关性分析表明MBN,TN与Acidobacteria,Actinobacteria呈显著正相关（P<0.05）,MBN和TN是大狼毒扩张样地土壤微生物群落变化的主要驱动因素。本研究结果为了解滇西北山地草甸大狼毒入侵草地根际土壤微生物多样性及其土壤微生物群落的环境响应特征奠定基础。

关键词: 大狼毒, 入侵, 山地草甸, 土壤养分, 土壤微生物

Abstract: Euphorbia jolkinii is a poisonous weed widely distributed in the mountain meadows of northwest Yunnan. Its unchecked expansion has caused a serious impact on the local grassland ecosystem. To investigate the impact of the expansion of Euphorbia jolkinii on the soil microenvironment of degraded mountain grasslands in northwest Yunnan, in this study we selected three distinct patch types based on the patchy distribution of Euphorbia jolkinii in degraded grassland communities： uninvasive patches, low coverage patches （8.9% Euphorbia jolkinii） and high coverage patches （38.2% Euphorbia jolkinii）. Soil samples were taken to determine the nutrient content from different patches, and the effects of Euphorbia jolkinii expansion on soil microbial community composition, structure, and diversity were analyzed. The results showed that the expansion of Euphorbia jolkinii significantly increased soil nutrient content, and the invasion of Euphorbia jolkinii into patch soil resulted in a significant increase in TN, SOC, MBC, MBN, MBP, AN, AP, and AK content （P<0.05）. The invasion of Euphorbia jolkinii significantly changed the composition and structure of soil bacterial communities, significantly increased the diversity of soil bacterial communities and the richness of fungal communities （P<0.05）. The response of soil bacterial and fungal communities to the spread and expansion of Euphorbia jolkinii was inconsistent. The results of correlation analysis showed that MBN and TN were significantly positively correlated with Acidobacteria and Actinobacteria （P<0.05）, and MBN and TN were the main driving factors for changes in soil microbial communities in the expansion plot of Euphorbia jolkinii. The results of this study lay a foundation for understanding the environmental response characteristics of soil microbial diversity and soil microbial communities in the rhizosphere of invasive grassland in the mountain meadow of northwest Yunnan Province.

Key words: Euphorbia jolkinii, Invasion, Mountain meadow, Soil nutrients, Soil microorganism

中图分类号:

S812.6

杨欣, 肖雪, 牛琼梅, 肖伟, 段蕊, 次旺, 侯晓晗, 初晓辉, 单贵莲. 大狼毒入侵对香格里拉山地草甸土壤微生物特征的影响[J]. 草地学报, 2025, 33(3): 778-788.

YANG Xin, XIAO Xue, NIU Qiong-mei, XIAO Wei, DUAN Rui, CI Wang, HOU Xiao-han, CHU Xiao-hui, SHAN Gui-lian. Effects of Euphorbia jolkinii Invasion on Soil Microbial Characteristics in Shangri La Mountain Meadows[J]. Acta Agrestia Sinica, 2025, 33(3): 778-788.

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参考文献

[1] LI J Z,LIU Y M,MO C H,et al. IKONOS image-based extraction of the distribution area of Stellera chamaejasme L. in Qilian County of Qinghai Province, China[J]. Remote Sensing, 2016, 8(2):148-149
[2] ZHAO B Y,LIU Z Y,LU H,et al. Damage and control of poisonous weeds in western grassland of China[J]. Agricultural Sciences in China,2010,9(10):1512-1521
[3] HARRIS R B. Rangeland degradation on the Qinghai-Tibetan Plateau:a review of the evidence of its magnitude and causes[J].Journal of Arid Environments,2010,74(1):1-12
[4] INDERJIT,VAN DER PUTTEN W H. Impacts of soil microbial communities on exotic plant invasions[J]. Trends in Ecology& Evolution,2010,25(9):512-519
[5] PUTTEN W H V D,BARDGETT R D,BEVER J D,et al. Plant-soil feedbacks:the past, the present and future challenges[J]. Journal of Ecology,2013,101(2):265-276
[6] TORRES N,HERRERA I,FAJARDO L,et al. Meta-analysis of the impact of plant invasions on soil microbial communities[J]. BMC Ecology and Evolution,2021,21(1):172
[7] SUN G,LUO P,WU N,et al. Stellera chamaejasme L. increases soil N availability,turnover rates and microbial biomass in an alpine meadow ecosystem on the eastern Tibetan Plateau of China[J]. Soil Biology and Biochemistry,2009,41(1):86-91
[8] CHENG J N,JIN H,ZHANG J L,et al. Effects of allelochemicals soil enzyme activities and environmental factors on rhizosphere soil microbial community of Stellera chamaejasme L. along a growth-coverage gradient[J]. Microorganisms,2022,10(1):158
[9] WANG Y Q, SONG M L, ZHOU R, et al. The effects of Ligularia flavescens on degraded alpine grassland plants, soil nutrients, and microbial biomass[J]. Journal of Grassland Science, 2022, 31(12):31-40
[10] NIU H B,LIU W X,WAN F H,et al. An invasive Aster(Ageratina adenophora) invades and dominates forest understories in China:altered soil microbial communities facilitate the invader and inhibit natives[J]. Plant and Soil,2007,294(1)73-85
[11] 高金会,王然,宋振,等.少花蒺藜草入侵对根际土壤磷细菌群落多样性的影响[J].生物安全学,2022,31(4):327-335
[12] REN B H,MENG M,YU J X,et al. Invasion by Cenchrus spinifex changes the soil microbial community structure in a sandy grassland ecosystem[J]. Heliyon,2023,9(11):e20860
[13] PIMENTEL D,LACH L,ZUNIGA R,et al. Environmental and economic costs of nonindigenous species in the United States[J]. BioScience,2000,50(1):53-65
[14] LIAO C Z,PENG R H,LUO Y Q,et al. Altered ecosystem carbon and nitrogen cycles by plant invasion:a meta-analysis[J]. New Phytologist,2008,177(3):706-714
[15] ZHAO Y H,MEMMOTT J,VAUGHAN I P,et al. The impact of a native dominant plant Euphorbia jolkinii on plant-flower visitor networks and pollen deposition on stigmas of co-flowering species in subalpine meadows of Shangri-La SW China[J]. Journal of Ecology,2021,109(5):2107-2120
[16] IWATA H,ITO T,KOKUBUGATA G,et al. The complete chloroplast genome of a coastal plant Euphorbia jolkinii(Euphorbiaceae)[J]. Mitochondrial DNA Part B,Resources,2022,7(3):569-570
[17] LEE S H,TANAKA T,NONAKA G I,et al. Structure and biogenesis of jolkinin a highly oxygenated ellagitannin from Euphorbia jolkinii[J].Journal of Natural Products,2004,67(6):1018-1022
[18] YAN Z Q,GUO H R,YANG J Y,et al. Phytotoxic flavonoids from roots of Stellera chamaejasme L.(Thymelaeaceae)[J]. Phytochemistry,2014,106:61-68
[19] ZHANG R H,QU S M,ZHANG B,et al. Interactive effects between the invasive weed Stellera chamaejasme and grass:can arbuscular mycorrhizal fungi and fungal pathogens coregulate interspecific relationships?[J]. Frontiers in Microbiology,2023,14:1236891
[20] YANG Y L,WANG J L,CHEN Y,et al. Remote-sensing monitoring of grassland degradation based on the GDI in shangri-La,China[J]. Remote Sensing,2019,11(24):3030
[21] 李嘉懿,初晓辉,尹海燕,等.大狼毒型亚高山草甸植被演替对围封的响应[J].中国草地学报2021,43(6):10-16
[22] 马祖艳.基于生理生化及代谢组学的大狼毒化感作用机制研究[D].昆明:云南农业大学,2023:9-56
[23] 单贵莲,马祖艳,李嘉懿,等.大狼毒对紫花苜蓿幼苗生理及内源激素含量的影响[J].草业学报,2023,32(10):153-161
[24] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科学技术出版社,2000:44-57
[25] 牛琼梅,单贵莲,罗钦,等.毒害草入侵扩散对滇西北亚高山草甸土壤微生物多样性的影响[J].草地学报,2023,31(7):1996-2004
[26] LIU Y X,XU J W,JIN Y H,et al. Relationship between herbaceous plant invasion and soil properties in the Changbai Mountain Tundra[J]. Acta Ecologica Sinica,2017,38(4):1235-1244
[27] ZHANG T,FU W D,ZHANG R H,et al. Effects of invasive Cenchrus spinifex on nitrogen pools in sandy grassland[J]. The Journal of Applied Ecology,2017,28(5):1522-1532
[28] CHE R X, LIU D, QIN J L, et al. Increased litter input significantly changed the total and active microbial communities in degraded grassland soils[J]. Soils Sediments,2020(20):2804-2816
[29] 沈聪,刘爽,苏建宇,等.半干旱荒漠区柠条根际细菌群落结构与功能[J].基因组学与应用生物学,2021,40(11):3508-3517
[30] 王雪莹,包新光,张峰,等.荒漠植物红砂根际土壤细菌群落特征及土壤酶活性研究[J].草地学报,2024,32(12):3764-3773
[31] WANG H,HE X,ZHANG Z F,et al. Eight years of manure fertilization favor copiotrophic traits in paddy soil microbiomes[J]. European Journal of Soil Biology,2021,106:103352
[32] HOU Y P,LIU L,CHU H,et al. Effects of exotic plant Rhus typhinainvasion on soil properties in different forest types[J]. Acta Ecologica Sinica,2015,35(16):5324-5330
[33] ADRIAN H,PAOLO D L D,BODELIER P L E. Revisiting life strategy concepts in environmental microbial ecology[J]. Fems Microbiology Ecology, 2017, 93(3):1093
[34] WU J P, LIU Z F, WANG X L,et al. Effects of understory removal and tree girdling on soil microbial community composition and litter decomposition in two Eucalyptus plantations in South China[J]. Functional Ecology,2011(25):921-931
[35] 于建新.少花蒺藜草入侵对土壤氮转化关键微生物群落的影响及其对氮添加的响应[D].沈阳:沈阳农业大学,2023:36-38
[36] LEKBERG Y,GIBBONS S M,ROSENDAHL S,et al. Severe plant invasions can increase mycorrhizal fungal abundance and diversity[J]. The ISME Journal,2013,7(7):1424-1433
[37] 郭倩,王博,樊勇明,等.半灌木扩张草原土壤细菌群落特征及其影响因素[J].草地学报,2024,32(5):1410-1419
[38] CHANG E H,CHIU C Y. Changes in soil microbial community structure and activity in a cedar plantation invaded by moso bamboo[J]. Applied Soil Ecology,2015(91):1-7
[39] 王桔红,张丽娜,陈学林,等.入侵植物对根际土壤微生物群落影响的研究进展[J].生态科学, 2016,35(6):204-210
[40] FURTAK K,GAŁĄZKA A,NIEDŹWIECKI J. Changes in soil enzymatic activity caused by hydric stress[J]. Polish Journal of Environmental Studies, 2020(4):2653-2660
[41] GUAN F, TANG X, FAN S, et al. Changes in soil carbon and nitrogen stocks followed the conversion from secondary forest to Chinese fir and moso bamboo plantations[J]. Catena,2015,133:455-460
[42] SARATHCHANDRA S U,GHANI A,YEATES G W,et al. Effect of nitrogen and phosphate fertilisers on microbial and nematode diversity in pasture soils[J]. Soil Biology and Biochemistry,2001,33(7/8):953-964
[43] DAI Y, YAN Z, JI J, et al. Soil bacteria diversity in rhizosphere under two types of vegetation restoration based on high throughput sequencing[J]. Acta Pedologica Sinica,2017(3):735-748

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大狼毒入侵对香格里拉山地草甸土壤微生物特征的影响

Effects of Euphorbia jolkinii Invasion on Soil Microbial Characteristics in Shangri La Mountain Meadows

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