草地学报

›› 2003, Vol. 11 ›› Issue (3): 219-222.DOI: 10.11733/j.issn.1007-0435.2003.03.006

• 研究论文 • 上一篇    下一篇

多年生黑麦草组织培养与植株再生研究

张万军, 王涛   

  1. 中国农业大学农业与生物技术国家重点实验室, 北京, 100094
  • 收稿日期:2002-12-10 修回日期:2003-04-25 出版日期:2003-08-15 发布日期:2003-08-15
  • 作者简介:张万军(1976- ),男,内蒙古凉城县人,硕士;王涛,研究员,为本文通讯作者,主要从事植物基因工程研究,Email:cauwangtao@vip.sina,com

A Study on the Tissue Culture and Plant Regeneration of Perennial Ryegrass

ZHANG Wan-jun, WANG Tao   

  1. National Laboratory for Agriculture and Biotechnology, China Agriculture University, Beijing, China 100094
  • Received:2002-12-10 Revised:2003-04-25 Online:2003-08-15 Published:2003-08-15

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摘要： 对多年生黑麦草种子为外植体的植株再生过程进行系统研究,结果表明,在改良的MS培养基(MSM),以MS无机盐+9.9mg/L维生素B1+9.5mg/L维生素B6+4.5mg/L尼克酸+1mg/LCH+30g/L蔗糖+琼脂8g/L为基本成分,附加5mg/L2,4-D和0.05mg/LKT时,适合种子的愈伤组织诱导;继代培养基附加2mg/L2,4-D和0.1mg/LKT;分化培养基附加1mg/L2,4-D和1mg/LKT;生根培养基为无激素的MS培养基。完成植株再生约需12周,愈伤组织分化率为70%。

关键词: 生物技术, 多年生黑麦草, 组织培养, 再生

Abstract: A systemic study of plant regeneration of perennial ryegrass has been conducted.The result showed the modified MS medium (MSM) that composed of MS salt +9.9 mg/L vitamin B1+9.5 mg/L vitamin B6+4.5 mg/L nicotinic acid+1 mg/L hydrolyze casine+30 g/L sucrose+8 g/L agar as basic medium was suitable for inducing callus formation from ryegrass seeds when 5 mg/L 2,4-D and 0.05 mg/L KT added.The subculture medium containing 2 mg/L 2,4-D and 0.1 mg/L KT.The medium for callus differentiation into plantlets containing 1 mg/L 2,4-D and 1 mg/L KT.The medium for regenerated plantlets rooting was basic MS medium free of hormone.The whole period of ryegrass plant regeneration takes about 12 weeks.The differentiation ratio of calli reached 70%.

Key words: Biotechnology, Perennial ryegrass, Tissue culture, Plant regeneration

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