草地学报

›› 2005, Vol. 13 ›› Issue (4): 287-290.DOI: 10.11733/j.issn.1007-0435.2005.04.006

• 研究报告 • 上一篇    下一篇

紫花苜蓿根瘤菌种子处理剂的研究

韦革宏, 朱毓华, 万晓红, 杨雪颖   

  1. 西北农林科技大学生命科学学院, 西北农林科技大学微生物研究中心, 陕西杨凌, 712100
  • 收稿日期:2005-02-05 修回日期:2005-05-09 出版日期:2005-11-15 发布日期:2005-11-15
  • 作者简介:韦革宏(1969- ),男,教授,博士,主要从事根瘤菌多样性及其应用研究,Email:weigehong@yahoo.com.cn
  • 基金资助:
    全国优秀博士学位论文作者基金(200254);教育部新世纪优秀人才支持计划基金(NCET-04-0953);教育部回国人员基金;西北农林科技大学拔尖人才支持计划基金

Study of Rhizobia Seed Treatment Formulations for Alfalfa

WEI Ge-hong, ZHU Yu-hua, WAN Xiao-hong, YANG Xue-ying   

  1. College of Life Sciences, Research Center of Microbiology, Northwest A & F University, Yangling, Shanxi Province, 712100, China
  • Received:2005-02-05 Revised:2005-05-09 Online:2005-11-15 Published:2005-11-15

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摘要： 研究不同拌种处理对紫花苜蓿(Medicago sativa)的影响,结果表明:室内与室外盆栽发芽结果基本一致,发芽率都没有明显提高;在苜蓿分枝期拌种处理的结瘤数和鲜重都有不同程度的增加,根瘤数量提高21.7%-232%,鲜重增加了3.7%-25.4%,光合速率和蒸腾速率略低于对照;苜蓿种子处理剂活菌量(CFUml-1)跟踪监测结果表明,S型最适合活菌保存,在常温保存条件(20-30℃)优于4℃保存。

关键词: 紫花苜蓿, 根瘤菌, 拌种

Abstract: A study of the formulations of alfalfa rhizobia seed dressing was conducted.Six groups of alfalfa seeds were mixed with three different treatment formulations.The result shows that there has been no obvious difference in germination rate between potted plants kept in or out of the experiment house.During the alfalfa plants branching period,their root nodule numbers per plant,weight of resh plant,photosynthesis rate,transpiration rate,and water use efficiency were measured.Comparing alfalfa plants grown from seed with or without rhizobia treatment,we found that the former produced more root nodules and attained higher fresh plant weight.The number of root nodules was raised 21.7%-23.2%,and the weight of fresh plants increased by 3.7%-25.4%,while the photosynthesis rate and the transpiration rate are lower than CK.The CFU ml-1 of seed treatment formulations was monitored.Among the three seed treatment formulations,S formulation was the fittest for preservation of live rhizobia.The preservation temperature of 20-30℃ was better than cold storage of 4℃.

Key words: Alfalfa, Rhizobia, Seed dressing

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