燕麦种子蛋白质组的GeLC-MS/MS分析

doi:10.11733/j.issn.1007-0435.2012.01.018

›› 2012, Vol. 20 ›› Issue (1): 108-115.DOI: 10.11733/j.issn.1007-0435.2012.01.018

燕麦种子蛋白质组的GeLC-MS/MS分析

王霞霞^1,2, 柴守玺¹, 常磊¹, 柴继宽¹, 徐智明²

1. 甘肃农业大学, 甘肃兰州730030;
2. 安徽农科院, 安徽合肥230031

收稿日期:2011-09-21 修回日期:2011-12-05 出版日期:2012-02-15 发布日期:2012-07-06
作者简介:王霞霞(1977- ),女,甘肃庆阳人,博士研究生,主要从事作物生态生理的研究,E-mail:wangxiaxia1029@sina.com
基金资助:
安徽省博士后基金资助

GeLC-MS/MS Analysis of Oat Seed Proteome

WANG Xia-xia^1,2, CHAI Shou-xi¹, CHANG Lei¹, CHAI Ji-kuan¹, XU Zhi-ming²

1. Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Anhui Academy of Agricultural Sciences, Hefei, Anhui Province 230031, China

Received:2011-09-21 Revised:2011-12-05 Online:2012-02-15 Published:2012-07-06

摘要/Abstract

摘要： 采用十二烷基磺酸钠-聚丙烯酰胺凝胶(SDS-PAGE)电泳结合液相色谱串联质谱方法(GeLC-MS/MS)对燕麦(Avena sativa L.)品种白燕2号种子不同溶解性的蛋白质进行分离鉴定,以期建立其种子蛋白质表达谱,为探讨干燥燕麦种子储藏及萌发的生理生化机理提供基础。结果表明:GeLC-MS/MS方法鉴定出溶解于超纯水、2.5%NaCl和70%乙醇的燕麦种子蛋白质分别为40,33和15个,共54个非冗余蛋白,其中29个蛋白至少可溶于2种溶剂。通过生物信息学软件对鉴定蛋白的分子功能、细胞组成和生物学功能进行预测,其中40个蛋白质功能得到明确的预测,涉及细胞过程、刺激应答及酶调节活性等12类。这些蛋白质与燕麦种子成熟干燥时期生命活动有关,为进一步从蛋白质水平探索燕麦种子生理代谢机理提供了理论依据。

关键词: 燕麦, 蛋白质组, 质谱

Abstract: For exploring the mechanism of biochemistry,the proteins of dry oat seeds(Avena sativa L.’baiyan No.2’)were extracted with MillQ water,2.5% NaCl solution and 70% ethanol then identified using SDS-PAGE and mass spectrometery approach(LC-MS/MS).Results showed that fifty-four proteins were identified by LC-MS/MS,hereinto;twenty-nine proteins could be dissolved in two or three kinds of solutions.Forty,thirty-three and fifteen proteins could be dissolved in MillQ water,2.5% NaCl solution and 70% ethanol,respectively.The molecular function,cell components and biological pathways of fifty-four proteins were predicted by GO database.The protein functions of forty proteins were identified involving in twelve major categories including stimulate response,enzyme regulator activity,cellular processes and so on.These proteins affected the activity of dried ripe oat seed.These results provided information to explore the biochemistry mechanism of oat seeds at the protein level in the future.

Key words: Oat, Proteome, Mass spectrometry

中图分类号:

王霞霞, 柴守玺, 常磊, 柴继宽, 徐智明. 燕麦种子蛋白质组的GeLC-MS/MS分析[J]. , 2012, 20(1): 108-115.

WANG Xia-xia, CHAI Shou-xi, CHANG Lei, CHAI Ji-kuan, XU Zhi-ming. GeLC-MS/MS Analysis of Oat Seed Proteome[J]. , 2012, 20(1): 108-115.

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参考文献

[1] Wilkins M R,Pasquali C,Appel R D.From proteins to proteome:large scale protein identification by two-dimensional electrophoresis and amino acid analysis[J].Nature Biotechnology,1996,14:61-65
[2] Mak Y,Skylas D J,Willows R,et al.A proteomic approach to the identification and characterization of protein composition in wheat germ[J].Functional & Integrative Genomics,2006,6:322-337
[3] Skylas D J,Mackintosh J A,Cordwell S J,et al.Proteome approach to the characterisation of protein composition in the developing and mature wheat grain endosperm[J].Journal of Cereal Science,2000,32:169-188
[4] Bak-Jensen K S,Laugesen S,Roepstorff P,et al.Two-dimensional gel electrophoresis pattern (pH 6-11) and identification of water-soluble barley seed and malt proteins by mass spectrometry[J].Proteomics,2004,4:728-742
[5] (O)stergaard O,Finnie C,Laugesen S,et al.Proteome analysis of barley seeds:Identification of major proteins from two-dimensional gels (pI 4-7)[J].Proteomics,2004,4:2437-2447
[6] Vensel W H,Tanaka C K,Cai N,etal.Developmental changes in the metabolic protein profiles of wheat endosperm[J].Proteomics,2005,5:1594-1611
[7] Xie Z S,Wang J Q,Cao M L,et al.Pedigree analysis of an elite rice hybrid using proteomic approach[J].Proteomics,2006,6(2):474-486
[8] Natarajan S S,XU C P,Bae H,et al.Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis[J].Journal of Agricultural and Food Chemistry,2006,54(8):3114-3120
[9] 李春梅,杨守萍,盖钧镒,等.野生大豆与栽培大豆种子差异蛋白质组学研究[J].生物化学与生物物理进展,2007,34(12):1296-1302
[10] Neuhoff V,Arold N,Taube N,et al.Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250[J].Electrophoresis,1988,9(6):255-262
[11] Shotwell M A,Boyer S K,Chesnut R S,et al.Analysis of seed storage protein genes of oats[J].Biological Chemistry,1990,265(17):9652-9658
[12] 蔺瑞,张美莉,张家超.裸燕麦球蛋白的分离纯化及其抗氧化活性研究[J].食品科学,2011,32(1):31-34
[13] 刘刚,刘英,陈季旺,等.燕麦粉蛋白的理化性质研究[J].食品科学,2008,29(6):70-74
[14] Bewley J D.Seed germination and dormancy[J].The Plant Cell,1997,9:1055-1066
[15] 何大澄,肖雪媛.差异蛋白质组学及其应用[J].北京师范大学学报,2002,38(4):558-562
[16] Finnie C,Melchior S,Roepstorff P,et al.Proteome analysis of grain filling and seed maturation in barley[J].Plant Physiology,2002,129:1308-1319
[17] Finnie C,Bak-Jensen K S,Laugesen S,et al.Differential appearance of isoforms and cultivar variation in protein temporal profiles reveals in the maturing barley grain proteome[J].Plant Science,2006,170:808-821
[18] Finnie C,Maeda K,Ostergaard,O,etal.Aspects of the barley seed proteome during development and germination[J].Biochemical Society Transactions,2004,32:517-519
[19] Chmelik J,Rehulka P,Stáelcová M,et al.proteomic analysis of different extracts from barley grains[J].Forestry,2002,48(6):261-264
[20] Gallardo K,Job C,Groot S P C,et al.Proteomic analysis of Arabidopsis seed germination and priming[J].Plant Physiology,2001,126:835-848

燕麦种子蛋白质组的GeLC-MS/MS分析

GeLC-MS/MS Analysis of Oat Seed Proteome

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