蒙古冰草MwDREB3基因的克隆及表达分析

doi:10.11733/j.issn.1007-0435.2015.02.024

草地学报 ›› 2015, Vol. 23 ›› Issue (2): 377-382.DOI: 10.11733/j.issn.1007-0435.2015.02.024

蒙古冰草MwDREB3基因的克隆及表达分析

赵彦^1,2,3, 陈雪英^1,2, 石凤敏^1,2, 云锦凤^1,2,3, 王俊杰^1,2,3

1. 内蒙古农业大学生态环境学院, 内蒙古呼和浩特 010018;
2. 内蒙古自治区草品种育繁工程技术研究中心, 内蒙古呼和浩特 010018;
3. 草地资源教育部重点实验室, 内蒙古呼和浩特 010018

收稿日期:2014-07-16 修回日期:2014-12-22 出版日期:2015-04-15 发布日期:2015-03-25
通讯作者: 云锦凤
作者简介:赵彦(1976-),女,内蒙古通辽人,博士,讲师,主要从事牧草遗传与育种研究,E-mail:zhaoyannmg@sina.com
基金资助:
国家自然科学基金(31160479);国家自然科学基金(31260578);内蒙古草业重大专项“优质牧草种质资源创新与产业化示范”(20120245)资助

Cloning and Expression Analysis of MwDREB3 from Mongolian Wheatgrass

ZHAO Yan^1,2,3, CHEN Xue-ying^1,2, SHI Feng-min^1,2, YUN Jin-feng^1,2,3, WANG Jun-jie^1,2,3

1. College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
2. Engineering Center of Technical Research on Cultivation and Propagation of Grass Varieties, Hohhot, Inner Mongolia 010018, China;
3. Key Laboratory of Grassland Resources (IMAU), Ministry of Education, Hohhot, Inner Mongolia 010018, China

Received:2014-07-16 Revised:2014-12-22 Online:2015-04-15 Published:2015-03-25

摘要/Abstract

摘要：

以蒙古冰草(Agropyron mongolicum)为研究材料,利用同源序列法克隆得到1个DREB3类转录因子基因,命名为MwDREB3,采用实时荧光定量RT-PCR技术分析MwDREB3 基因的表达特征,为蒙古冰草优异抗性基因的挖掘和利用提供理论依据.结果表明:该基因全长1056bp,包含1个完整的开放阅读框,长度为774bp,编码257个氨基酸,该蛋白近5'端含有1个保守的AP2结构域.系统进化树分析表明MwDREB3 编码的蛋白与小麦(Triticum aestivum)的DREB、山羊草(Aegilops columnaris)DRF1的进化关系较近.该基因在干旱、高盐、ABA诱导条件下,表达量上调;在低温条件下,表达量下调.

关键词: 蒙古冰草, DREB, 基因克隆, 表达分析

Abstract:

To explore and exploit the excellent resistance gene resources of Mongolian wheatgrass(Agropyron Mongolicum Keng), A DREB3 transcription factor gene was cloned by homologous sequences from the Mongolian wheatgrass, named MwDREB3 (1056bp), and the open reading frame was 774bp, encoding 257 amino acids, containing a conserved AP2 domain nearby the 5'end. Phylogenetic analysis revealed that MwDREB3 had a closer relationship with DREBs from wheat (Triticum aestivum) and Aegilops (Aegilops columnaris). Expression patterns of MwDREB3 gene were analyzed using real-time quantitative RT-PCR, the MwDREB3 expression was up under the conditions of drought, high salt and ABA induction, and was down under the low temperatures.

Key words: Mongolian wheatgrass, DREB, Cloning, Expression analysis

中图分类号:

Q944.58
S54

赵彦, 陈雪英, 石凤敏, 云锦凤, 王俊杰. 蒙古冰草MwDREB3基因的克隆及表达分析[J]. 草地学报, 2015, 23(2): 377-382.

ZHAO Yan, CHEN Xue-ying, SHI Feng-min, YUN Jin-feng, WANG Jun-jie. Cloning and Expression Analysis of MwDREB3 from Mongolian Wheatgrass[J]. Acta Agrestia Sinica, 2015, 23(2): 377-382.

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蒙古冰草MwDREB3基因的克隆及表达分析

Cloning and Expression Analysis of MwDREB3 from Mongolian Wheatgrass

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