草地学报 ›› 2015, Vol. 23 ›› Issue (3): 448-452.DOI: 10.11733/j.issn.1007-0435.2015.03.002

• 前沿研究 • 上一篇    下一篇

蒙古冰草Afa家族串联重复序列克隆及染色体定位

赵彦1,2, 窦全文3, 云锦凤1,2, 王俊杰1,2   

  1. 1. 内蒙古农业大学生态环境学院, 内蒙古 呼和浩特 010018;
    2. 草地资源教育部重点实验室, 内蒙古 呼和浩特 010018;
    3. 中科院西北高原生物研究所, 青海 西宁 810008
  • 收稿日期:2014-05-26 修回日期:2014-10-11 出版日期:2015-06-15 发布日期:2015-07-02
  • 通讯作者: 云锦凤
  • 作者简介:赵彦(1976-),女,内蒙古呼和浩特人,博士,讲师,主要从事牧草遗传与育种研究,E-mail: zhaoyannmg@sina.com
  • 基金资助:

    国家自然科学基金(31160479)(31260578);内蒙古草业重大专项“优质牧草种质资源创新与产业化示范”(20120245)资助

Isolating and location of Tandem Repetitive Afa-family Sequences from Mongolian Wheatgrass (Agropyron mongolicum Keng)

ZHAO Yan1,2, DOU Quan-wen3, YUN Jin-feng1,2, WANG Jun-jie1,2   

  1. 1. Ecology and environment college of Inner Mongolia agricultural University (IMAU), Huhhot, Inner Mongolia 010019, China;
    2. Key Laboratory of Grassland Resources (IMAU), Ministry of Education, Hohhot, Inner Mongolia 010019, China;
    3. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810008, China
  • Received:2014-05-26 Revised:2014-10-11 Online:2015-06-15 Published:2015-07-02
  • Supported by:

    This work was supported by National Nature Science Foundation of China (No.31260578,No. 31160479)

摘要:

Afa家族串联重复序列因只出现在小麦及小麦族近缘属物种而得名,本研究从蒙古冰草中克隆得到一个Afa家族序列,长度为233 bp,命名为pAmAfa1,该序列在GENBANK中进行同源序列比对,结果表明该序列与大多数小麦族其他物种的Afa家族串联重复序列存在较高的相似性;系统进化分析表明,蒙古冰草pAmAfa1序列与大赖草pLrAfa3,pLrAfa5序列聚在一起,表明蒙古冰草P染色体组与大赖草的N、X染色体亲源关系较近。为了明确Afa家族串联重复序列在蒙古冰草染色体上的位置,双色荧光原位杂交技术被采用,以pAmAfa1为探针检测到杂交信号出现在染色体的末端或近端部的区域,每条染色体上都有杂交信号,表明Afa家族串联重复序列普遍存在于P染色体组中。

关键词: 蒙古冰草, Afa家族串联重复序列, 克隆, 荧光原位杂交

Abstract:

Tandem repetitive Afa-family sequences are known to occur in the wheat and related species of Triticeae. A member of the Afa-family sequences isolated from Mongolian Wheatgrass (Agropyron mongolicum Keng) (2n=2x=14,PP) is 233 bp, named as pAmAfa1, which is most similar with other Afa-family sequences in Triticeae species. The phylogenic analysis results show that pAmAfa1 is clustered with pLrAfa3 and pLrAfa5, and the findings indicate that P genome has homology with N and X genomes. To know which the genome of Mongolian wheatgrass carries the Afa-family sequence; FISH is carried out using pAmAfa1 as the probe. The signals appear in the telomeric regions and subtelomeric regions of all chromosomes. This finding indicates that P genomes contain Afa-family repeats.

Key words: Mongolian Wheatgrass, Tandem repetitive Afa-family sequences, Molecular Cloning, FISH

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