PEG引发对老化燕麦种子抗氧化性能的影响

doi:10.11733/j.issn.1007-0435.2016.05.001

草地学报 ›› 2016, Vol. 24 ›› Issue (5): 933-938.DOI: 10.11733/j.issn.1007-0435.2016.05.001

• 专论与进展 • 下一篇

PEG引发对老化燕麦种子抗氧化性能的影响

夏方山^1,2, 毛培胜², 王明亚², 闫慧芳², 侯龙鱼², 陈玲玲², 朱艳乔², 汪辉²

1. 山西农业大学动物科技学院, 山西太谷 030801;
2. 中国农业大学动物科技学院草业科学北京市重点实验室, 北京 100193

收稿日期:2015-10-12 修回日期:2016-03-16 出版日期:2016-10-15 发布日期:2017-01-13
通讯作者: 毛培胜
作者简介:夏方山(1983-),男,山东安丘人,博士,研究方向为草类植物育种与种子科学,E-mail:dqlxfs8583@163.com
基金资助:
博士点基金项目（20110008110003）；国家“十二五”科技支撑课题“优质多抗牧草新品种选育与良种繁育关键技术研究与示范（2011BAD17B01-02）”资助

Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds

XIA Fang-shan^1,2, MAO Pei-sheng², WANG Ming-ya², YAN Hui-fang², HOU Long-yu², CHEN Ling-ling², ZHU Yan-qiao², WANG Hui²

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. College of Animal Science and Technology, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China

Received:2015-10-12 Revised:2016-03-16 Online:2016-10-15 Published:2017-01-13

摘要/Abstract

摘要：

试验以45℃老化48 d的超干（含水量4%）燕麦（Avena sativa）种子为材料，通过不同浓度PEG-6000（0，-0.3，-0.6，-0.9和-1.2 Mpa）引发0（CK），3和6 h后，分析其超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性及丙二醛（MDA）含量的变化，以探讨PEG引发对老化的超干燕麦种子抗氧化性能的影响。结果表明：浓度为0和-0.3 Mpa的PEG引发会降低SOD，CAT和POD的活性，导致MDA含量增加，从而降低老化燕麦种子发芽率；而高浓度（-0.9和-1.2 Mpa）的PEG引发则相反。说明适当浓度的PEG引发主要通过提高超干燕麦种子的抗氧化能力来修复其老化损伤，并避免其吸胀损伤。

关键词: 燕麦, 引发, 聚乙二醇, 种子老化, 超干

Abstract:

This study was designed to analyze the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and malondialdehyde (MDA) content in ultra-dried (4% moisture content) oat (Avena sativa) seeds. The seeds were deteriorated at 45℃ for 48 d, and then primed with different concentrations of PEG-6000 (0, -0.3, -0.6, -0.9 and -1.2 Mpa) for 0 (CK), 3 and 6 h. This study was to explore the effect of PEG priming on physiological responses of aged ultra-dry oat seeds. The results showed that the activities of SOD, CAT and POD decreased in aged oat seeds primed with PEG at the concentration of 0 and -0.3 Mpa, leading to an increase in MDA content and decrease in germination percentage. However, the opposite trend was observed after priming with high concentration of PEG (-0.9 and -1.2 Mpa). This indicated that PEG priming repaired the damage of ageing and prevented the imbibition damage in ultra-dried oat seeds by improving antioxidant capacity.

Key words: Oat, Priming, Polyethylene glycol, Seed ageing, Ultra-dry

中图分类号:

S512.6

夏方山, 毛培胜, 王明亚, 闫慧芳, 侯龙鱼, 陈玲玲, 朱艳乔, 汪辉. PEG引发对老化燕麦种子抗氧化性能的影响[J]. 草地学报, 2016, 24(5): 933-938.

XIA Fang-shan, MAO Pei-sheng, WANG Ming-ya, YAN Hui-fang, HOU Long-yu, CHEN Ling-ling, ZHU Yan-qiao, WANG Hui. Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds[J]. Acta Agrestia Sinica, 2016, 24(5): 933-938.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2016.05.001

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2016/V24/I5/933

参考文献

[1] DraganicI, LekicS. Seed priming with antioxidants improves sunflower seed germination and seedling growth under unfavorable germination conditions[J]. Turkisk Journal of Agriculture and Forestry,2012,36(4):421-428
[2] Parkhey S, Naithani S C, Keshavkant S. ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging[J]. Plant Physiology and Biochemistry,2012,57(1):261-267
[3] Perez-Garcia F, Gomez-Campo C, Ellis R H. Successful long-term ultra-dry storage of seed of 15 species of Brassicaceae in a genebank:variation in ability to germinate over 40 years and dormancy[J]. Seed science and technology[J]. 2009,37(3):640-649
[4] Li Y, Qu J J, Zhang W M, et al. Impact of ultra-dry storage on vigor capacity and antioxidant enzyme activities in seed of Ammopiptanthus mongolica[J]. Botanical Studies,2010,51(4):465-472
[5] Cui K, Wang H Y, Li K, et al. Physiological and biochemical effects of ultra-dry storage on barbados nut seeds[J]. Crop Science,2014,54(4):1748-1755
[6] Bewley J D, Bradford K J, Hilhorst H W M, et al. Seeds:Physiology of development, germination and dormancy[M]. 3^rd Edi. Springer Press,New York,2013:143-147
[7] 张自阳,姜小苓,卢春瑞,等. PEG引发处理对人工老化小麦种子活力及幼苗生长的影响[J]. 河南科技学院学报,2013,41(1):1-5
[8] 王炜,史雨刚,王曙光. PEG引发对老化大豆种子发芽及活力的影响[J]. 山西农业科学,2011,39(7):650-654
[9] 潘春柳,黄燕芬,邓志军,等. PEG处理对新鲜和老化穿心莲种子萌发及幼苗生长的影响[J]. 种子,2013,32(12):30-34
[10] 王慧超,赵昌琼,郑美娜,等. 聚乙二醇(PEG)浸种对茎瘤芥老化种子活力及其幼苗生长的影响[J]. 植物生理学通讯,2010,46(2):131-134
[11] 冯世鑫,马小军,李小勇,等. PEG浸种对老化的蔓性千斤拔种子萌发及活力的影响[J]. 作物杂志,2013(1):136-139
[12] 张春平,何平,杜丹丹,等. ZnSO₄和PEG引发对老化紫苏种子萌发及幼苗抗氧化酶活性的影响[J]. 中国中药杂志,2010,35(18):2372-2377
[13] Jafari H, Najafi R, Soltani A, et al. Seed deterioration and seedling germination of soybean (Glycine max L.) cv. Sahar[J]. International Journal of Agronomy and Plant Production,2013,4(SI):3762-3766
[14] 夏方山,闫慧芳,毛培胜,等. PEG引发对燕麦老化种子萌发与幼苗生长的影响[J]. 草业科学,2015,32(5):731-737
[15] 覃方锉,赵桂琴,焦婷,等. 含水量及添加剂对燕麦捆裹青贮品质的影响[J]. 草业学报,2014,23(6):119-125
[16] Shewry P R, Piironen V, Lampi A M, et al. Phytochemical and fiber components in oat varieties in the HEALTHGRAIN diversity screen[J]. Journal of Agricultural and Food Chemistry,2008,56(21):9777-9784
[17] ISTA. International Rules for Seed Testing[M]. Bassersdorf:Zurich,2013
[18] Rao K V M, Sresty T V S. Antioxidant parameters in the seedlings of pigeon pea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses[J]. Plant Science,2000,157(1):113-128
[19] Kalpana R, Madhava, Rao K V. On the aging mechanism in pigeon pea (Cajanus cajan L.) seed[J]. Seed Science and Technology,1995,23(1):1-9
[20] Bailly C, Benamar A, Corbineau F, et al. Changes in malondialdehyde content and superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging[J]. Physiologia Plantarum,1996,97(1):104-110
[21] McDonald M B. Seed deterioration:physiology, repair and assessment[J]. Seed Science and Technolgy,1999,27(1):177-237
[22] 王曙光,赵建奎,宁幸莲,等. PEG-6000引发对老化大豆种子膜透性及保护性酶活性的影响[J]. 华北农学报,2012,27(6):113-117
[23] 张海旺,芦翠乔,吴丁,等. 聚乙二醇(PEG)渗透处理对老化油菜种子过氧化及细胞膜透性的影响[J]. 华北农学报,1989,4(2):56-62
[24] Bewley J D, Bradford K J, Hilhorst H W M, et al. Seeds:Physiology of development, germination and dormancy[M]. 3^rd.New York:Springer,2013
[25] 宋松泉,程红焱,姜孝成. 种子生物学[M]. 北京:科学出版社,2008
[26] 顾龚平,吴国荣,陆长梅,等. PEG处理对大豆幼苗活力及活性氧代谢的影响[J]. 中国油料作物学报,2000,22(2):26-30
[27] 张凤银,陈禅友,胡志辉. PEG-6000胁迫对宿根天人菊种子萌发及幼苗生理特性的影响[J]. 西北农林科技大学学报(自然科学版),2014,42(1):132-136

PEG引发对老化燕麦种子抗氧化性能的影响

Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	宋词, 黄小涛, 石国玺, 周华坤, 王芳萍, 姚步青. 播期对青藏高原燕麦生长和饲草品质的影响[J]. 草地学报, 2021, 29(S1): 121-127.
[2]	吴海艳, 曲珍, 刘昭明, 拉巴顿珠, 同桑措姆, 曲尼, 尼玛卓嘎, 马玉寿. 基于主成分分析的燕麦品种生产性能的比较研究[J]. 草地学报, 2021, 29(9): 1967-1973.
[3]	朱海霞. 多孢木霉HZ-31菌株胁迫下野燕麦内参基因的选择[J]. 草地学报, 2021, 29(8): 1666-1674.
[4]	童永尚, 鱼小军, 徐长林, 汪鹏斌, 宋建超, 李珍, 李颖. 天祝高寒区播期对7个燕麦品种饲草产量及品质的影响[J]. 草地学报, 2021, 29(5): 1094-1106.
[5]	夏方山, 王聪聪, 李红玉, 刘曼, 郑川, 张耀丹, 范华芳. 硒引发对紫花苜蓿种子抗氧化性能的影响[J]. 草地学报, 2021, 29(3): 472-477.
[6]	梁国玲, 刘文辉, 马祥. 590份皮燕麦种质资源穗部性状遗传多样性分析[J]. 草地学报, 2021, 29(3): 495-503.
[7]	刘备, 宋玉梅, 孙铭, 毛培胜. 燕麦劣变种子吸胀过程中线粒体AsA-GSH循环的生理响应[J]. 草地学报, 2021, 29(2): 211-219.
[8]	贾志锋, 马祥, 琚泽亮, 刘凯强, 赵桂琴. 施氮量和播种量对燕麦光合特性、激素含量及种子产量的影响[J]. 草地学报, 2021, 29(2): 293-302.
[9]	张琦, 魏臻武, 闫天芳, 耿小丽. 燕麦种质资源农艺性状遗传多样性的鉴定评价[J]. 草地学报, 2021, 29(2): 309-316.
[10]	李文, 魏廷虎, 阿保地, 永措巴占, 才仁塔次, 周玉海, 李文浩, 郭卫兴, 张雁平. 化肥减施配合有机肥对高寒区燕麦营养品质和土壤养分的影响[J]. 草地学报, 2021, 29(12): 2878-2886.
[11]	胡佳燕, 刘畅, 李志坚, 周帮伟, 白静. 不同类型皮燕麦在吉林省西部的产量与农艺性状的比较研究[J]. 草地学报, 2021, 29(10): 2247-2257.
[12]	汪雪, 刘晓静, 赵雅姣, 王静. 紫花苜蓿/燕麦间作对燕麦碳、氮代谢及其物质积累的影响研究[J]. 草地学报, 2021, 29(10): 2258-2264.
[13]	周磊, 王璐, 赵宝平, 米俊珍, 王凤梧, 刘景辉, 张丹, 刘烨焜. 北方农牧交错区不同播期和刈割期对燕麦饲草产量和品质的影响[J]. 草地学报, 2021, 29(10): 2355-2363.
[14]	付东青, 王彦超, 宋磊, 王旭哲, 张凡凡, 马春晖. 施氮量和种植密度对石河子复播早熟饲用燕麦生产性能的影响[J]. 草地学报, 2021, 29(10): 2364-2371.
[15]	贾志锋, 马祥, 琚泽亮, 刘凯强, 赵桂琴. 氮肥和种植密度对燕麦叶片衰老特性及细胞结构的影响[J]. 草地学报, 2021, 29(1): 80-87.