黄梁木叶精油对柱花草和水稻秸秆发酵品质及细菌群落的影响

doi:10.11733/j.issn.1007-0435.2024.10.035

草地学报 ›› 2024, Vol. 32 ›› Issue (10): 3326-3334.DOI: 10.11733/j.issn.1007-0435.2024.10.035

• 技术研发 • 上一篇

黄梁木叶精油对柱花草和水稻秸秆发酵品质及细菌群落的影响

周雨歆, 杨丹, 杨伟涛, 陈丹丹, 陈晓阳, 张庆

华南农业大学林学与风景园林学院/广东木本饲料工程技术研究中心/广东省森林植物种质创新与利用重点实验室, 广东广州 510642

收稿日期:2024-05-28 修回日期:2024-07-03 发布日期:2024-11-04
通讯作者: 张庆,E-mail:zqing1988@126.com
作者简介:周雨歆(2000-)，女，汉族，江西南昌人，硕士研究生，主要从事牧草青贮加工研究，E-mail：Deodatus@163.com
基金资助:
广东长隆慈善基金会(CLPF2021007Z);国家自然科学基金项目(31771345);广东省林业科技创新项目(2019KJCX001)资助

Effect of Neolamarckia cadamba Leaf Essential Oil on Fermentation Quality and Bacterial Community of Stylo and Rice Straw Silage

ZHOU Yu-xin, YANG Dan, YANG Wei-tao, CHEN Dan-dan, CHEN Xiao-yang, ZHANG Qing

College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong Province 510642, China

Received:2024-05-28 Revised:2024-07-03 Published:2024-11-04

摘要/Abstract

摘要： 为探究黄梁木(Neolamarckia cadamba)叶精油分别对柱花草(Stylosanthes guianensis L.)和水稻(Oryza sativa L.)秸秆青贮发酵品质和细菌群落结构的影响，本试验基于新鲜柱花草和水稻秸秆重量的0.5%和1%添加黄梁木叶精油(NCEO)进行青贮混合处理，每个处理3组重复，装入聚乙烯密封袋并用真空封口机密封，青贮30 d后测定样品的青贮发酵品质指标，分析细菌群落结构。结果表明：添加黄梁木叶精油显著降低了柱花草和水稻秸秆青贮饲料中pH值、氨态氮含量、乳酸菌和大肠菌群数量(P＜0.05)，显著提高了水稻秸秆青贮饲料的乳酸和真蛋白含量(P＜0.05)。乳酸菌在水稻秸秆青贮饲料中占优势地位，且数量随黄梁木叶精油添加量的增加而升高。黄梁木叶精油的添加也增加了异根瘤菌属、甲基杆菌属和黄单胞杆菌属的相对丰度。综上所述，添加1%黄梁木叶精油可有效改善柱花草和水稻秸秆青贮发酵品质。

关键词: 黄梁木叶精油, 青贮品质, 细菌群落, 柱花草, 水稻秸秆

Abstract: The objective of this experiment was to explore the effect of Neolamarkia cadamba leaf essential oil (NCEO) on the fermentation quality and bacterial community of stylo (Stylosanthes guianensis L.) and rice(Oryza sativa L.) straw silage. Based on the weight of fresh stylo and rice straw,adding the 0.5% and 1% NCEO into materials for 30 days ensiling. Vacuumed and sealed after three replicates of each treatment were packed into plastic bags (20 cm×30 cm). After 30 days of ensiling,the chemical composition and silage indexes of the samples were evaluated statistically. The results of fermentation parameter,protein fraction and bacterial community indicated that NCEO addition decreased (P<0.05) pH value,ammonia-N content,and numbers of lactic acid bacteria and coliform bacteria both in stylo and rice straw silage. And lactic acid content and true protein proportion of rice straw silage increased (P<0.05) with NCEO addition. Lactobacillus was the most dominant genus and increased with NCEO addition in rice straw silage. The NCEO addition also increased the relative abundance of Allorhizobium,Methylobacterium and Xanthomonas. In conclusion,the 1% NCEO addition was an effective way to improve the fermentation of stylo and rice straw silage.

Key words: Neolamarckia cadamba leaf essential oil, Fermentation quality, Bacterial community, Stylo, Rice straw

中图分类号:

S816.5+3

周雨歆, 杨丹, 杨伟涛, 陈丹丹, 陈晓阳, 张庆. 黄梁木叶精油对柱花草和水稻秸秆发酵品质及细菌群落的影响[J]. 草地学报, 2024, 32(10): 3326-3334.

ZHOU Yu-xin, YANG Dan, YANG Wei-tao, CHEN Dan-dan, CHEN Xiao-yang, ZHANG Qing. Effect of Neolamarckia cadamba Leaf Essential Oil on Fermentation Quality and Bacterial Community of Stylo and Rice Straw Silage[J]. Acta Agrestia Sinica, 2024, 32(10): 3326-3334.

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参考文献

[1] LI M,ZHOU H,ZI X,et al. Silage fermentation and ruminal degradation of stylo prepared with lactic acid bacteria and cellulase[J]. Animal Science Journal,2017,88(10):1531-1537
[2] 黄乃馨,李雪枫,展小霞,等. 含水量对柱花草青贮品质和微生物群落动态的影响[J]. 草地学报,2024,55(1):232-244
[3] PEN M,SAVAGE D B,NOLAN J V,et al. Effect of stylosanthes guianensis supplementation on intake and nitrogen metabolism of Bos indicus cattle offered a basal diet of mixed rice straw and tropical grass[J]. Animal Production Science,2013,53(5):453-457
[4] NGUYEN V H,TOPNO S,BALINGBING C,et al. Generating a positive energy balance from using rice straw for anaerobic digestion[J]. Energy Reports,2016,2:117-122
[5] ZHAO J,DONG Z,LI J,et al. Ensiling as pretreatment of rice straw:the effect of hemicellulase and Lactobacillus plantarum on hemicellulose degradation and cellulose conversion[J]. Bioresource Technology,2018,266:158-165
[6] 王成,王益,周玮,等. 植物乳杆菌和含水量对辣木叶青贮品质和单宁含量的影响[J]. 草业学报,2019,28(6):109-118
[7] WANG C,ZHENG M,WU S,et al. Effects of gallic acid on fermentation parameters,protein fraction,and bacterial community of whole plant soybean silage[J]. Frontiers in Microbiology,2021,12:662966
[8] LIU Q,ZHANG J,SHI S,et al. The effects of wilting and storage temperatures on the fermentation quality and aerobic stability of stylo silage[J]. Animal Science Journal,2011,82(4):549-553
[9] SHINOZAKI Y,KITAMOTO H K. Ethanol production from ensiled rice straw and whole-crop silage by the simultaneous enzymatic saccharification and fermentation process[J]. Journal of Bioscience and Bioengineering,2011,111(3):320-325
[10] SILVA L D D,PEREIRA O G,SILVA T C D,et al. Effects of silage crop and dietary crude protein levels on digestibility,ruminal fermentation,nitrogen use efficiency,and performance of finishing beef cattle[J]. Animal Feed Science and Technology,2016,220:22-33
[11] 杨丹,吴硕,陈丹丹,等. 黄梁木精油活性成分测定及其对全株玉米青贮有氧稳定的影响[J]. 草地学报,2023,31(9):2860-2867
[12] SU G,ZHOU X,WANG Y,et al. Dietary supplementation of plant essential oil improves growth performance,intestinal morphology and health in weaned pigs[J]. Journal of Animal Physiology and Animal Nutrition,2020,104(2):579-589
[13] 张德安,杨若渚,刘杰,等. 饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析[J]. 畜牧兽医学报,2024,55(1):232-244.
[14] WANG C,PIAN R,CHEN X,et al. Beneficial effects of tannic acid on the quality of bacterial communities present in high-moisture mulberry leaf and stylo silage[J]. Frontiers in Microbiology,2020,11:586412
[15] KE W,DING Z,LI F,et al. Effects of malic or citric acid on the fermentation quality,proteolysis and lipolysis of alfalfa silage ensiled at two dry matter contents[J]. Journal of Animal Physiology and Animal Nutrition,2022,106(5):988-994
[16] ZHANG Q,YU Z,YANG H,et al. The effects of stage of growth and additives with or without cellulase on fermentation and in vitro degradation characteristics of Leymus chinensis silage[J]. Grass and Forage Science,2016,71(4):595-606
[17] Official methods of analysis of AOAC International[J]. Choice Reviews Online,1997,35(2):128
[18] VANSOEST P J,ROBERTSON J B,LEWIS B A. Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science,1991,74(10):3583-3597
[19] MURPHY R P. A method for the extraction of plant samples and the determination of total soluble carbohydrates[J]. Journal of the Science of Food and Agriculture,1958,9(11):714-717
[20] LAZZARI G,POPPI A C O,MACHADO J,et al. Effects of protein source and lipid supplementation on conservation and feed value of total mixed ration silages for finishing beef cattle[J]. Journal of Animal Science,2021,99(2):1-13
[21] CHEN D,ZHENG M,GUO X,et al. Altering bacterial community:A possible way of lactic acid bacteria inoculants reducing CO₂ production and nutrient loss during fermentation[J]. Bioresource Technology,2021,329:124915
[22] YANG H,WANG B,ZHANG Q,et al. Improvement of fermentation quality in the fermented total mixed ration with oat silage[J]. Microorganisms,2021,9(2):420
[23] NI K,WANG X,LU Y,et al. Exploring the silage quality of alfalfa ensiled with the residues of astragalus and hawthorn[J]. Bioresource Technology,2020,297:122249
[24] SMITH L H. Theoretical carbohydrate requirement for alfalfa silage production[J]. Agronomy Journal,1962,54(4):291-293
[25] WANG Y,WANG C,ZHOU W,et al. Effects of wilting and lactobacillus plantarum addition on the fermentation quality and microbial community of moringa oleifera leaf silage[J]. Frontiers in Microbiology,2018,9:1817
[26] CAI Y,BENNO Y,OGAWA M,et al. Influence of lactobacillus spp. from an inoculant and of weissella and leuconostoc spp. from forage crops on silage fermentation[J]. Applied and Environmental Microbiology,1998,64(8):2982-2987
[27] KUNG L J,SMITH M L,BENJAMIM D S E,et al. An evaluation of the effectiveness of a chemical additive based on sodium benzoate,potassium sorbate,and sodium nitrite on the fermentation and aerobic stability of corn silage[J]. Journal of Dairy Science,2018,101(7):5949-5960
[28] MCDONALD P,HENDERSON A,HERON S. The biochemistry of silage[M]. 2nd ed. Marlow:Chalcombe publications,1991:9-12
[29] SEGLAR B. Fermentation analysis and silage quality testing[D]. Minneapolis,Minnesota:University of Minnesots,2003:126
[30] HE L,CHEN N,LV H,et al. Gallic acid influencing fermentation quality,nitrogen distribution and bacterial community of high-moisture mulberry leaves and stylo silage[J]. Bioresource Technology,2020,295:122255
[31] PANG H,QIN G,TAN Z,et al. Natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype,16 s ribosomal RNA and recA gene analysis[J]. Systematic and Applied Microbiology,2011,34(3):235-241
[32] PAHLOW G,MUCK R E,DRIEHUIS F,et al. Microbiology of ensiling[J]. Silage Science and Technology,2003,42:31-93
[33] DONG Z,SHAO T,LI J,et al. Effect of alfalfa microbiota on fermentation quality and bacterial community succession in fresh or sterile Napier grass silages[J]. Journal of Dairy Science,2020,103(5):4288-4301
[34] LIU B,HUAN H,GU H,et al. Dynamics of a microbial community during ensiling and upon aerobic exposure in lactic acid bacteria inoculation-treated and untreated barley silages[J]. Bioresource Technology,2019,273:212-219
[35] DUNIERE L,SINDOU J,CHAUCHEYRAS-DURAND F,et al. Silage processing and strategies to prevent persistence of undesirable microorganisms.[J]. Animal Feed Science and Technology,2013,182(1/4):1-15
[36] ZHENG M L,NIU D Z,JIANG D,et al. Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar[J]. Journal of Applied Microbiology,2017,122(6):1456-1470
[37] NI K,ZHAO J,ZHU B,et al. Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum[J]. Bioresource Technology,2018,265:563-567
[38] PLENGVIDHYA V,BREIDT F J,LU Z,et al. DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations[J]. Applied and Environmental Microbiology,2007,73(23):7697-7702
[39] ZHAI Y,PEREZ-DIAZ I M. Contribution of Leuconostocaceae to CO₂-mediated bloater defect in cucumber fermentation[J]. Food Microbiology,2020,91:103536
[40] DUTKIEWICZ J,MACKIEWICZ B,LEMIESZEK M K,et al. Pantoea agglomerans:a mysterious bacterium of evil and good. Part.Ⅳ. Beneficial effects[J]. Annals of Agricultural and Environmental Medicine：Aaem,2016,23(2):206-222
[41] OGUNADE I M,JIANG Y,CERVANTES A A P,et al. Bacterial diversity and composition of alfalfa silage as analyzed by illumina Miseq sequencing:effects of Escherichia coli O157:H7 and silage additives[J]. Journal of Dairy Science,2018,101(3):2048-2059
[42] HOLLAND M. Methylobacterium and plants[J]. Recent Res Dev in Plant Physiol,1997,1:207-213
[43] GREEN P N. About this rererence work[J].Bergey's Manual,2015,7:60608
[44] TURGUT A,BASIM H. Development of a real-timePCR assay using locked nucleic acid probe for the detection of octopine,nopaline,and vitopine strains of tumorigenic Allorhizobium vitis in grapevines[J]. Crop Protection,2021,145:105620
[45] WICHMANN F,HUG B M,WIDMER F,et al. Phenotypic and molecular genetic characterization indicate no major race-specific interactions between Xanthomonas translucens pv. Graminis and lolium multiflorum[J]. Plant Pathology,2011,60(2):314-324

黄梁木叶精油对柱花草和水稻秸秆发酵品质及细菌群落的影响

Effect of Neolamarckia cadamba Leaf Essential Oil on Fermentation Quality and Bacterial Community of Stylo and Rice Straw Silage

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