不同组培基质对菊苣叶片再生的影响

doi:10.11733/j.issn.1007-0435.2014.01.033

›› 2014, Vol. 22 ›› Issue (1): 208-212.DOI: 10.11733/j.issn.1007-0435.2014.01.033

不同组培基质对菊苣叶片再生的影响

张玉, 白史且, 李达旭

四川省草原科学研究院, 成都犀浦 611743

收稿日期:2013-05-24 修回日期:2013-07-03 出版日期:2014-02-15 发布日期:2014-01-28
通讯作者: 白史且，E-mail：baiturf@yahoo.com.cn
作者简介:张玉（1975-），女，四川仁寿人，博士，主要从事牧草育种，E-mail：zhyforage@126.com
基金资助:
四川省科技厅应用基础项目（2013JY0111）；“十二五”牧草育种攻关（2011NZ0098-11）；国家“十二五”科技支撑计划（2011BAD17B03）资助

Effects of Different Culture Media on the Leaf Regeneration of Chicory

ZHANG Yu, BAI Shi-qie, LI Da-xu

Sichuan Academy of Grassland Science, Xipu, Chengdu 611731, China

Received:2013-05-24 Revised:2013-07-03 Online:2014-02-15 Published:2014-01-28

摘要/Abstract

摘要： 以3个菊苣（Cichorium intybus L.）品种叶片为外置体，以MS，N6，B5，SH和N6B5MS为基本培养基，添加不同激素对其不定芽诱导及植株再生进行研究。结果表明：3个菊苣品种在5种培养基上的愈伤诱导率和分化率存在差异，其中以MS培养基添加2.0 mg·L^-16-BA和0.2 mg·L^-1 IBA再生和分化最好，平均愈伤诱导率和分化率分别为70.12%和72.07%。在不同生根培养基中，生根率和根长存在一定的差异，生根数量没有显著差异，其中以1/2MS培养基中添加0.1 mg·L^-1的NAA效果最显著，生根率、每个外植体的平均根数和根长分别达到97.07%，4.03个和6.51 cm。

关键词: 培养基, 菊苣, 再生

Abstract: Efficient adventitious shoot induction and plant regeneration of young leaves from three chicory cultivars on MS, N6, B5, SH and N6B5MS media with different hormone concentrations were studied in this paper. Results showed that average callus induction rate and differentiation rate had significant difference among three chicory cultivars on five media. The best medium was MS supplemented 2.0 mg·L^-1 6-BA and 0.2 mg·L^-1 IBA, average callus induction rate and differentiation rate were 70.12% and 72.07%, respectively. Rooting rate and length had significant difference between MS and 1/2MS media, whereas the number of root had no significant difference. The best root medium was 1/2MS supplemented 0.1 mg·L^-1NAA, Rooting rate, the length and number of root were 97.07%, 6.51 cm and 4.03, respectively.

Key words: Medium, Chicory, Regeneration

中图分类号:

Q943.1

张玉, 白史且, 李达旭. 不同组培基质对菊苣叶片再生的影响[J]. , 2014, 22(1): 208-212.

ZHANG Yu, BAI Shi-qie, LI Da-xu. Effects of Different Culture Media on the Leaf Regeneration of Chicory[J]. , 2014, 22(1): 208-212.

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不同组培基质对菊苣叶片再生的影响

Effects of Different Culture Media on the Leaf Regeneration of Chicory

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