外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响

doi:10.11733/j.issn.1007-0435.2020.03.001

草地学报 ›› 2020, Vol. 28 ›› Issue (3): 589-596.DOI: 10.11733/j.issn.1007-0435.2020.03.001

• 研究论文 • 下一篇

外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响

普布卓玛^1,2, 其美拉姆¹, 李丹丹¹, 多吉顿珠², 张靖¹, 孙运府¹, 苗彦军³, 付娟娟¹, 呼天明¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西藏自治区农科院草业科学研究所, 西藏拉萨 850000;
3. 西藏农牧学院植物科学学院, 西藏林芝 860000

收稿日期:2019-12-09 修回日期:2020-02-16 出版日期:2020-06-15 发布日期:2020-05-30
通讯作者: 付娟娟, 呼天明
作者简介:普布卓玛(1991-),女,藏族,西藏拉萨人,硕士研究生,主要从事植物逆境生物学研究,E-mail:puzhuo050509@nwafu.edu.cn;
基金资助:
国家自然科学基金项目（31901382）；西北农林科技大学科研启动经费项目（2452017195）；中国博士后科学基金项目（2018M631208）；国家自然科学基金项目（31872411）；国家牧草产业技术体系拉萨综合试验站项目（CARS-34）共同资助

Effects of Exogenous Proline on Growth and Antioxidant Enzyme Related Gene Expression of Elymus nutans under Low Temperature Stress

PUBU Zhuo-ma^1,2, QIMEI La-mu¹, LI Dan-dan¹, DUOJI Dun-zhu², ZHANG Jing¹, SUN Yun-fu¹, MIAO Yan-jun³, FU Juan-juan¹, HU Tian-ming¹

1. Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. Tibet Academy of Agriculture and Animal Husbandry Sciences, Lasa, Tibet 850000, China;
3. College of Plant Science, Agricultural and Animal Husbandry College of Tibet University, Linzhi, Tibet 860000, China

Received:2019-12-09 Revised:2020-02-16 Online:2020-06-15 Published:2020-05-30

摘要/Abstract

摘要： 本试验以西藏野生垂穗披碱草（Elymus nutans Griseb.）为研究对象，采用盆栽试验，研究叶面喷施外源脯氨酸（10 mM）对低温胁迫（4℃）下垂穗披碱草幼苗生长及抗氧化酶活性及其基因表达的影响。结果表明：低温胁迫下脯氨酸处理能够显著提高垂穗披碱草幼苗的根长、地上和地下鲜重、地上和地下干重，与低温胁迫相比分别增加27.11%，48.80%，48.83%，51.74%，38.87%；外源脯氨酸增加了低温胁迫下内源脯氨酸和可溶性糖的积累，分别增加42.96%和22.98%（P<0.05）；而且脯氨酸处理提高了APX、CAT、ChICu/ZnSOD、CytCu/ZnSOD基因的表达水平以及过氧化物酶（Peroxidase，POD）、过氧化氢酶（Catalase，CAT）、超氧化物歧化酶（Superoxide dismutase，SOD）和抗坏血酸过氧化物酶（Ascorbate peroxidase，APX）活性，降低了丙二醛（Malondialdehyde，MDA）含量和相对电导率水平（P<0.05）。综上所述，脯氨酸能够增强低温胁迫下垂穗披碱草幼苗的渗透调节能力及抗氧化酶活性，维持细胞膜的稳定性，缓解其生长抑制，从而提高垂穗披碱草的抗寒性。

关键词: 脯氨酸, 低温胁迫, 垂穗披碱草, 抗氧化酶, 基因表达

Abstract: The wild Elymus nutans in Tibet was used as a test material,and 10 mM proline was sprayed on the leaves of E. nutans to investigate the physiological responses and the expression of antioxidant enzyme-related genes of plants in response to cold stress (4℃). The results showed that proline treatment significantly increased the root length (27.11%),aboveground fresh (48.80%) and dry weight (48.83%),and underground fresh and dry weight (51.74% and 38.87%) of E. nutans under low temperature stress (4℃). Meanwhile,pretreatment with proline increased proline and soluble sugar contents by 42.96% and 22.98% (P<0.05),respectively;increased the relative expression level of APX,CAT,ChICu/ZnSOD, and CytCu/ZnSOD genes. Antioxidant enzymes activities of POD,CAT,SOD,and APX were also improved (P<0.05) by proline treatment. Proline treatment led to a decrease in MDA accumulation and relative conductivity by 47.84% and 20.87%,respectively (P<0.05). In summary,these findings suggest that proline regulates the stability of cell membrane structure through enhancing the ability of osmotic adjustment and antioxidant defense activities,thereby improves the cold tolerance of E. nutans.

Key words: Proline, Low temperature stress, Elymus nutans, Antioxidant enzyme, Gene expression

中图分类号:

普布卓玛, 其美拉姆, 李丹丹, 多吉顿珠, 张靖, 孙运府, 苗彦军, 付娟娟, 呼天明. 外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响[J]. 草地学报, 2020, 28(3): 589-596.

PUBU Zhuo-ma, QIMEI La-mu, LI Dan-dan, DUOJI Dun-zhu, ZHANG Jing, SUN Yun-fu, MIAO Yan-jun, FU Juan-juan, HU Tian-ming. Effects of Exogenous Proline on Growth and Antioxidant Enzyme Related Gene Expression of Elymus nutans under Low Temperature Stress[J]. Acta Agrestia Sinica, 2020, 28(3): 589-596.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2020.03.001

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2020/V28/I3/589

参考文献

[1] Janowiak F,Maas B,Dörffling K. Importance of abscisic acid for chilling tolerance of maize seedlings[J]. Journal of Plant Physiology,2002,159(6):635-643
[2] 陈默君,贾慎修. 中国饲用植物[M]. 北京:中国农业出版社,2002:119-120
[3] 舒必超,杨勇,刘雪勇,等. 低温胁迫对狗牙根生理及基因表达的影响[J]. 草业学报,2018,27(11):106-119
[4] 董文科,马祥,张玉娟,等. 低温胁迫对不同早熟禾品种糖酵解代谢及其相关基因表达的影响[J]. 草地学报,2019,27(06):1503-1510
[5] 王宁,袁美丽,陈浩,等. 干旱胁迫及复水对入侵植物节节麦幼苗生长及生理特性的影响[J]. 草业学报,2019,28(01):70-78
[6] 张海娜,鲁向晖,金志农,等. 高温条件下稀土尾砂干旱对4种植物生理特性的影响[J]. 生态学报,2019,39(07):2426-2434
[7] 项洪涛,齐德强,李琬,等. 低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响[J]. 草业学报,2019,28(04):81-94
[8] Oraee A,Tehranifar A,Nezami A,et al. Effects of drought stress on cold hardiness of non-acclimated viola (Viola×wittrockiana ‘Iona Gold with Blotch’) in controlled conditions[J]. Scientia Horticulturae,2018,238:98-106
[9] De Freitas,Paulo André Ferreira,De Carvalho H H,et al. Salt acclimation in sorghum plants by exogenous proline:physiological and biochemical changes and regulation of proline metabolism[J]. Plant Cell Reports,2019,38(3):403-416
[10] De Freitas,Paulo André Ferreira,Rafael D S M,et al. Salt Tolerance Induced by Exogenous Proline in Maize Is Related to Low Oxidative Damage and Favorable Ionic Homeostasis[J]. Journal of Plant Growth Regulation,2018,37(3):911-924
[11] Ryu J Y,Hong S Y,Jo S H,et al. Molecular and functional characterization of cold-responsive C-repeat binding factors from Brachypodium distachyon[J]. BMC Plant Biology,2014(14):15
[12] 王小华,庄南生. 脯氨酸与植物抗寒性的研究进展[J]. 中国农学通报,2008,24(11):398-402
[13] Jain R,Shrivastava A K,Solomon S,et al. Low temperature stress-induced biochemical changes affect stubble bud sprouting in sugarcane (Sacchaum spp. hybrid)[J]. Plant Growth Regulation,2007,53(1):17-23
[14] Beck E H,Heim R,et al. Plant resistance to cold stress:mechanisms and environmental signals triggering frost hardening and dehardening[J]. Journal of Biosciences,2004,29(4):449-459
[15] Fu J,Geng J,Miao Y,et al. Transcriptomic analyses reveal genotype-and organ-specific molecular responses to cold stress in Elymus nutans[J]. Biologia Plantarum,2018,62(4):671-683
[16] 李合生. 植物生理生化实验原理和技术[M]. 第2版.北京:高等教育出版社,2000:134-261
[17] Nakano Y,Asada K. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts[J]. Plant & Cell Physiology,1981,22(5):867-880
[18] Levin S A. The Problem of Pattern and Scale in Ecology[M]. Springer US:Ecological Time Series,1995:277-326
[19] Kj Livak,Td Schmittgen. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2^-△△Ct Method[J]. Methods,2000,25(4):402-408
[20] 赵慧,赵一博,郭江波,等. 植物耐受低温胁迫研究进展[J]. 种子,2017,36(5):47-50
[21] 曹慧明,史作民,周晓波,等. 植物对低温环境的响应及其抗寒性研究综述[J]. 中国农业气象,2010,31(2):310-314
[22] 董文科,马祥,周学文,等. 外源甜菜碱对低温胁迫下紫花苜蓿幼苗生理特性的影响[J]. 草地学报,2019,27(01):130-140
[23] 于凤玲,支庆祥. 低温对植物的危害以及植物的抗寒性[J]. 畜牧与饲料科学,2009,30(9):190-191
[24] 王宁. 植物抗寒生理性研究进展[J]. 北方园艺,2014(04):174-177
[25] 王芳,王淇,赵曦阳. 低温胁迫下植物的表型及生理响应机制研究进展[J]. 分子植物育种,2019,17(15):5144-5153
[26] 付娟娟,刘建,孙永芳,等. 冷胁迫对2种垂穗披碱草生长和生理特性的影响[J]. 草地学报,2014,22(04):789-795
[27] 史红梅,张桂香,张海燕,等. 低温胁迫对高粱幼苗脯氨酸积累的影响[J]. 山西农业科学,2014,42(09):945-947
[28] Ren Y,Miao M,Meng Y,et al. DFR1-Mediated Inhibition of Proline Degradation Pathway Regulates Drought and Freezing Tolerance in Arabidopsis[J]. Cell Reports,2018,23(13):3960-3974
[29] 陈贵,胡文玉. 提取植物体内MDA的溶剂及MDA作为衰老指标的探讨[J]. 植物生理学报,1991(1):44-46
[30] 李爱军,张桂香,周福平,等. 低温胁迫下高粱幼苗对外源脯氨酸的响应[J]. 种子,2019,38(05):44-47
[31] Delauney A J,Verma D P S. Proline biosynthesis and osmoregulation in plants[J]. Plant Journal for Cell & Molecular Biology,13,4(4):215-223
[32] Guerrier G. Proline Accumulation in Leaves of NaCl-sensitive and NaCl-tolerant Tomatoes[J]. Biologia Plantarum,1997,40(4):623-628
[33] 普布卓玛,罗艺岚,高金柱,等. 2,4表-油菜素内酯对低温胁迫下西藏野生垂穗披碱草幼苗抗氧化保护和渗透调节的影响[J]. 草地学报,2019,27(03):547-552
[34] 王玮,吴传万,王欣,等. 外源脯氨酸对盐胁迫下萝卜幼苗生长、抗氧化酶活性及渗透调节物质积累的影响[J]. 江西农业报,2019,31(03):51-56
[35] 刘书仁,郭世荣,孙锦,等. 脯氨酸对高温胁迫下黄瓜幼苗活性氧代谢和渗调物质含量的影响[J]. 西北农业学报,2010,19(04):127-131
[36] 舒必超,杨勇,刘雪勇,等. 低温胁迫对狗牙根生理及基因表达的影响[J]. 草业学报,2018,27(11):106-119
[37] Jiang Y,Watkins E,Liu S,et al. Antioxidative responses and candidate gene expression in prairie junegrass under drought stress[J].Journal of the American Society for Horticultural Science,2010,135(4):303-309

外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响

Effects of Exogenous Proline on Growth and Antioxidant Enzyme Related Gene Expression of Elymus nutans under Low Temperature Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	牟丹, 杨帆, 卡着才让, 祁学忠, 顾智辉, 谢久祥, 李希来. 高加索三叶草响应不同降温模式低温胁迫的代谢组学分析[J]. 草地学报, 2021, 29(9): 1877-1884.
[2]	王宝强, 赵颖, 朱晓林, 王旺田, 魏小红. 盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响[J]. 草地学报, 2021, 29(8): 1689-1696.
[3]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[4]	宋建超, 鱼小军, 魏孔涛, 汪鹏斌, 童永尚, 李珍, 贺有龙, 汪海波. 施氮对高寒区垂穗披碱草饲草生产性能及营养品质的影响[J]. 草地学报, 2021, 29(7): 1555-1564.
[5]	其美拉姆, 普布卓玛, 崔彤彤, 张新飞, 姜惠娜, 付娟娟, 呼天明. 外源钙对低温胁迫下西藏野生垂穗披碱草生理及相关基因表达的影响[J]. 草地学报, 2021, 29(5): 919-928.
[6]	秦金萍, 刘颖, 马玉寿, 王彦龙, 李世雄. 返青期休牧对退化高寒草地植被特征及优势种光合生理的影响[J]. 草地学报, 2021, 29(5): 1034-1042.
[7]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[8]	夏方山, 王聪聪, 李红玉, 刘曼, 郑川, 张耀丹, 范华芳. 硒引发对紫花苜蓿种子抗氧化性能的影响[J]. 草地学报, 2021, 29(3): 472-477.
[9]	李波, 李晨阳, 李红, 杨曌. 短期低温胁迫对‘龙牧807’苜蓿幼苗生理代谢的影响[J]. 草地学报, 2021, 29(3): 515-521.
[10]	刘备, 宋玉梅, 孙铭, 毛培胜. 燕麦劣变种子吸胀过程中线粒体AsA-GSH循环的生理响应[J]. 草地学报, 2021, 29(2): 211-219.
[11]	许超, 何承刚, 牟兰, 毕玉芬, 姜华. 干热胁迫下紫花苜蓿Rubisco羧化酶和活化酶活性变化及其基因表达的研究[J]. 草地学报, 2021, 29(2): 228-233.
[12]	张玉霞, 丛百明, 王显国, 张庆昕, 杜晓艳, 田永雷. 苜蓿抗寒性与根系抗氧化酶活性相关性分析[J]. 草地学报, 2021, 29(2): 244-249.
[13]	岳丽楠, 师尚礼, 祁娟, 杨培志, 刘刚, 刘文辉, 张英俊, 荆晶莹. 免耕补播对北方退化草地生产力及营养品质的影响[J]. 草地学报, 2021, 29(11): 2583-2590.
[14]	王升升, 段珍, 张吉宇. 发根农杆菌介导的白花草木樨毛状根转化体系的建立[J]. 草地学报, 2021, 29(11): 2591-2599.
[15]	姜惠娜, 敬松, 李晗玉, 佘木子, 张新飞, 呼天明, 付娟娟, 苗彦军. 西藏野生垂穗披碱草EnPLA1基因克隆与表达分析[J]. 草地学报, 2021, 29(10): 2141-2148.