Screening of Embryogenic Callus from Hybrid F1 of Caucasian Clover×White Clover

doi:10.11733/j.issn.1007-0435.2009.05.018

›› 2009, Vol. 17 ›› Issue (5): 650-654.DOI: 10.11733/j.issn.1007-0435.2009.05.018

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Screening of Embryogenic Callus from Hybrid F₁ of Caucasian Clover×White Clover

LUAN Xiao-min¹, WANG Ming-jiu¹, HE Li-jun², LI-Yao¹, LI Zhi-qiang¹

1. College of Ecology and Environmental Science, Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China;
2. College of Agronomy, Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China

Received:2009-03-24 Revised:2009-06-11 Online:2009-10-15 Published:2009-10-15

2种三叶草杂种F₁代胚性愈伤组织筛选

栾晓敏¹, 王明玖¹, 何丽君², 李耀¹, 李志强¹

1. 内蒙古农业大学生态环境学院, 呼和浩特, 010019;
2. 内蒙古农业大学农学院, 呼和浩特, 010019

通讯作者: 王明玖,E-mail:wangmj_0540@163.com
作者简介:栾晓敏(1982- ),女,蒙古族,内蒙古赤峰市人,硕士研究生,研究方向为草地生态及草地资源,E-mail:luan.xiao.min@163.com
基金资助:
国家自然科学基金(30660118),内蒙古教育基金(NJC07051)

Abstract

Abstract: The appropriate culture medium of callus inducement was screened using the adventitious shoots as explant from the stems of aseptic seedling of F₁ crossed between the embryos of Caucasian clover(Trifolium ambiguum Bieb.) and white clover(Trifolium.repens L.) by in vitro culture.The callus from stems of F₁ after successive transfer culture was transferred into differentiation medium and the effect of hormone combinations with different concentrations on the somatic embryogenesis was studied.The results show that hormone combination of MS+6-BA + NAA were the suitable medium for the callus inducement from stems of F₁ and MS+2,4-D+6-BA+NAA+KT+CH was the suitable media for the stem callus differentiation.Histological observation was performed on embryogenic callus after differentiation and the strumae structure were detected on the surface of callus.These strumae structures were embryogenic cell masses which could be further developed into the somatic embryo as the foundation of the rapid acquisition of regeneration plants of hybrid F₁.

Key words: Caucasian clover, White clover, Embryo culture in vitro, Hybrid F₁, Embryogenic callus

摘要： 以高加索三叶草(Trifolium ambiguum Bieb.)与白三叶(Trifolium.repens L.)杂交后胚离体培养产生的F₁无菌苗的茎段为外植体,筛选诱导愈伤组织的最适培养基,将F₁茎段产生的愈伤组织经过继代后转移到分化培养基中,研究不同浓度激素组合对体细胞胚胎产生的影响。结果表明:MS+6-BA+NAA是诱导F₁茎段愈伤组织的适宜培养基;MS+2,4-D+6-BA+NAA+KT+CH为F₁茎段愈伤组织的适宜分化培养基;对分化后的胚性愈伤进行组织学观察,可见其表面形成许多瘤状突起,即胚性细胞团,它们可以继续发育形成体细胞胚,为快速得到数量较多的杂种F₁再生苗奠定基础。

关键词: 高加索三叶草, 白三叶, 离体胚培养, 杂种F₁, 胚性愈伤组织

CLC Number:

S334.5

LUAN Xiao-min, WANG Ming-jiu, HE Li-jun, LI-Yao, LI Zhi-qiang. Screening of Embryogenic Callus from Hybrid F₁ of Caucasian Clover×White Clover[J]. , 2009, 17(5): 650-654.

栾晓敏, 王明玖, 何丽君, 李耀, 李志强. 2种三叶草杂种F₁代胚性愈伤组织筛选[J]. , 2009, 17(5): 650-654.

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Screening of Embryogenic Callus from Hybrid F₁ of Caucasian Clover×White Clover

2种三叶草杂种F₁代胚性愈伤组织筛选

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