Abstract: Elymus nutans Griseb. was used as the donor plant and Elymus sibiricus Linn. was used as the recipient plants to study the role of arbusculae mycorrhizal fungi (AMF) in plants resisting insect pests in this study. Funneliformis mosseae was selected as the inoculum. A total of 5 treatments，including the presence of hyphae but no root connection (A),sterile filaments and Root system connection (B),no root system but with mycelium connection and rotation before pest treatment (C),both mycelium and root system connection (D),and no inoculation but root system connection (E),were set up to measure AMF colonization,aboveground and belowground biomass,carbon and nitrogen content and defense enzymes. The results showed that， AM fungi significantly increased underground biomass of receiver plants(P<0.05);AM fungi induced a significant increase in defense enzyme activities in donor and receiver plants after the occurrence of insect pests(P<0.05). This experiment indicated that AMF not only promoted development growth of host plants,but also its mycelial network could act as a pathway for the transmission of defensive chemical signals between plants.

Key words: Elymus nutans Griseb, Arbuscular mycorrhizal fungi, Common mycelial network, Insect resistance

摘要： 为研究丛枝菌根真菌(Arbusculae mycorrhizal fungi，AMF)在植物抵御虫害过程中的作用，本研究以垂穗披碱草(Elymus nutans Griseb.)为供体植物，老芒麦(Elymus sibiricus Linn.)为受体植物，以摩西管柄囊霉(Funneliformis mosseae)作为接种菌种，共设置5个处理，即：存在菌丝，但无根系连接(A)、无菌丝和根系连接(B)、无根系但有菌丝连接，且在虫害处理前旋转(C)、既有菌丝又有根系连接(D)、无接菌但存在根系连接(E)，测定了菌根侵染率、地上地下生物量、碳氮含量以及防御酶活性等指标。结果表明：AM真菌显著提高了受体植物地下生物量(P<0.05)；虫害发生后，AM真菌诱导了供受体植物体内的防御酶活性显著升高(P<0.05)。本试验证明了AMF可以促进宿主植物的生长发育，菌丝网络则为主要防御化学信号的传递途径。

关键词: 垂穗披碱草, 丛枝菌根真菌, 菌丝网络, 抗虫防御