Identification of WRKY Family Genes and Expression Analysis under Alkaline Salt Stress in Bermudagrass

doi:10.11733/j.issn.1007-0435.2026.02.008

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 456-468.DOI: 10.11733/j.issn.1007-0435.2026.02.008

Identification of WRKY Family Genes and Expression Analysis under Alkaline Salt Stress in Bermudagrass

MA Shi-rui¹, YU Zheng-fa¹, YU Qi-kun¹, YU Rui¹, ZHU Li-li¹, ZHANG Zhi-yang¹, LI Pei-ying^1,2,3

1. College of Grassland science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
2. Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang 830052, China;
3. Key Laboratory of Grassland Resources and Ecology of the Ministry of Education of the Western Arid Zone, Urumqi, Xinjiang 830052, China

Received:2025-04-30 Revised:2025-08-02 Published:2026-01-22

狗牙根WRKY基因家族鉴定及碱性盐胁迫下表达分析

马诗睿¹, 于正发¹, 喻启坤¹, 于瑞¹, 朱莉莉¹, 张志扬¹, 李培英^1,2,3

1. 新疆农业大学草业学院, 新疆乌鲁木齐 830052;
2. 新疆草地资源与生态自治区重点实验室, 新疆乌鲁木齐 830052;
3. 西部干旱区草地资源与生态教育部重点实验室, 新疆乌鲁木齐 830052

通讯作者: 李培英，E-mail:lpy@xjau.edu.cn
作者简介:马诗睿（2001-），女，汉族，新疆昌吉人，硕士研究生，主要从事草种抗性分析机制研究，E-mail:1529837814@qq.com；
基金资助:
国家自然科学基金（32260341）；新疆维吾尔自治区重点研发项目（2023B02031-1）资助

Abstract

Abstract: Bermudagrass has important application value in landscaping and ecological restoration because of its strong drought resistance， saline-alkali resistance， and developed rhizomes and stolons. However， the molecular mechanism of its response to alkaline salt stress is still unclear. The WRKY gene family plays an important role in plant growth， development and defense against biotic and abiotic stresses， but it has not been fully identified in bermudagrass. Therefore， based on genomic data， this study systematically identified the physical and chemical properties， chromosome distribution， cis-acting elements， gene structure， conserved motifs and phylogenetic trees of WRKY gene family members in bermudagrass. At the same time， based on transcriptome data， the expression pattern of its family members under alkaline salt stress was analyzed. The results showed that 129 WRKY transcription factors were identified in the bermudagrass genome and named according to their location on the chromosome. Phylogenetic analysis was carried out by comparing with Arabidopsis WRKY protein， and the above transcription factors were divided into three families ： I， II and III， which were unevenly distributed on 18 chromosomes with the largest number of chromosomes 7 （22）.Collinearity analysis showed that fragment replication mainly drove the evolution of CdWRKYs in bermudagrass. Based on the transcriptome data， 11 differentially expressed CdWRKYs under alkaline salt stress were screened out. The qRT-PCR analysis showed that the expression levels of CdWRKY59， CdWRKY81， CdWRKY113 and CdWRKY126 reached the peak at 3 h， and their expression folds were 99.6-312.3 times than the control. It is speculated that these WRKY family members might play an important role in alkaline salt stress tolerance. The results of this study analyzed the role of WRKY genes in the response of bermudagrass to alkaline salt stress， and laid a foundation for further functional analysis of WRKY gene family.

Key words: Bermudagrass, WRKY transcription factor, Genome-wide identification, Alkaline salt stress, Expressive mode

摘要： 本研究基于狗牙根（Cynodon dactylon）基因组数据，采用生物信息学方法对其WRKY基因家族成员进行鉴定，同时基于转录组数据分析其在碱性盐胁迫下的表达模式。结果显示，狗牙根基因组中共鉴定出129个WRKY转录因子，根据其在染色体上的位置进行命名。通过与拟南芥WRKY蛋白比对进行系统发育分析，将上述转录因子分为Ⅰ、Ⅱ、Ⅲ共3个族，其不均匀分布在狗牙根18条染色体上，其中7号染色体的数量最多（22个）。共线性分析表明，片段复制主要驱动了狗牙根CdWRKYs的进化。基于转录组数据，筛选出11个碱性盐胁迫下差异表达的CdWRKYs，经qRT-PCR分析表明，推测CdWRKY59、CdWRKY81、CdWRKY113与CdWRKY126可能在狗牙根碱性盐胁迫耐受中具有重要作用。本研究结果可为WRKY基因家族的进一步功能分析奠定基础。

关键词: 狗牙根, WRKY转录因子, 全基因组鉴定, 碱性盐胁迫, 表达模式

MA Shi-rui, YU Zheng-fa, YU Qi-kun, YU Rui, ZHU Li-li, ZHANG Zhi-yang, LI Pei-ying. Identification of WRKY Family Genes and Expression Analysis under Alkaline Salt Stress in Bermudagrass[J]. Acta Agrestia Sinica, 2026, 34(2): 456-468.

马诗睿, 于正发, 喻启坤, 于瑞, 朱莉莉, 张志扬, 李培英. 狗牙根WRKY基因家族鉴定及碱性盐胁迫下表达分析[J]. 草地学报, 2026, 34(2): 456-468.

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Identification of WRKY Family Genes and Expression Analysis under Alkaline Salt Stress in Bermudagrass

狗牙根WRKY基因家族鉴定及碱性盐胁迫下表达分析

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