Cloning and Expression Analysis of the EnWRKY26 Gene in Elymus nutans Griseb

doi:10.11733/j.issn.1007-0435.2026.06.007

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (6): 2032-2042.DOI: 10.11733/j.issn.1007-0435.2026.06.007

Cloning and Expression Analysis of the EnWRKY26 Gene in Elymus nutans Griseb

WANG Jiao-feng, LIU Yu-cheng, YANG Shi-yu, HU Tian-ming, HAN Xiang-yan, YANG Pei-zhi

Department of Grassland Science, College of Grassland Agriculture, Northwest A& F University, Yangling, Shaanxi Province 712100, China

Received:2025-11-10 Revised:2026-01-12 Published:2026-06-02

垂穗披碱草EnWRKY26基因克隆及表达分析

王娇凤, 刘昱成, 杨世裕, 呼天明, 韩祥艳, 杨培志

西北农林科技大学草业与草原学院, 陕西杨凌 712100

通讯作者: 呼天明,E-mail:hutianming@126.com;韩祥艳,E-mail:xy_han@nwafu.edu.cn;杨培志,E-mail:yangpeizhi@126.com
作者简介:王娇凤(2001-),女,汉族,山西忻州人,硕士研究生,主要从事牧草逆境生物学研究,E-mail:wang_jiaofeng@126.com
基金资助:
国家自然科学基金青年科学基金项目（32401478）；国家自然科学基金-区域创新发展联合基金（U21A20240）；国家自然科学基金-区域联合发展重点基金（U20A2050）资助

Abstract

Abstract: WRKY transcription factors are widely involved in plant growth and development and play important roles in biotic and abiotic stress responses. In this study we cloned the EnWRKY26 gene from Elymus nutans Griseb ‘Ba Qing’ and conducted bioinformatics analysis， subcellular localization analysis， gene expression profiling， and transcription factor activity validation. The results indicated that EnWRKY26 was 996 bp in length， encoding 331 amino acids， with a molecular weight of 35 501.70 Da. The theoretical isoelectric point of the EnWRKY26 protein was 9.49， with an instability index of 53.78 and an average hydrophobicity of -0.652， indicating that it was an unstable hydrophilic protein. The entire protein sequence lacks transmembrane domains， and its secondary structure is predominantly composed of random coils. EnWRKY26 is most closely related to TraseCS7B02G418600 and TraseCS7D02G497700 in terms of phylogenetic relationship. Subcellular localization analysis revealed that EnWRKY26 was localized in the nucleus. Transcription factor activity validation confirmed that it possesses transcriptional activation activity. Under salt stress treatment， the relative expression level of EnWRKY26 showed an increasing trend. This study provides a theoretical basis for an in-depth understanding of the molecular mechanism by which EnWRKY26 regulates the response of Elymus nutans to salt stress.

Key words: Elymus nutans, EnWRKY26, Gene cloning, Expression analysis, Transcription factors

摘要： WRKY转录因子广泛参与植物的生长发育过程，并在响应生物和非生物胁迫中发挥重要作用，本研究从‘巴青’垂穗披碱草（Elymus nutans Griseb ‘Ba Qing’）中成功克隆了EnWRKY26基因，并对其进行了生物信息学分析、亚细胞定位分析、基因表达分析和转录因子活性验证等。结果表明：EnWRKY26全长996 bp，共编码331个氨基酸，分子量为35，501.70 Da；EnWRKY26蛋白理论等电点为9.49，不稳定系数为53.78，疏水性平均值为-0.652，是一种不稳定亲水性蛋白，整个蛋白序列无跨膜区域，二级结构以无规卷曲为主；EnWRKY26与小麦TraseCS7B02G418600和小麦TraseCS7D02G497700亲缘关系最近；亚细胞定位分析显示EnWRKY26存在于细胞核中；转录因子活性验证表明其具有转录激活活性；在盐胁迫处理下，EnWRKY26相对表达量呈现升高趋势。本研究为深入解析EnWRKY26调控垂穗披碱草响应盐胁迫的分子机制提供理论依据。

关键词: 垂穗披碱草, EnWRKY26, 基因克隆, 表达分析, 转录因子

CLC Number:

Q945.78

WANG Jiao-feng, LIU Yu-cheng, YANG Shi-yu, HU Tian-ming, HAN Xiang-yan, YANG Pei-zhi. Cloning and Expression Analysis of the EnWRKY26 Gene in Elymus nutans Griseb[J]. Acta Agrestia Sinica, 2026, 34(6): 2032-2042.

王娇凤, 刘昱成, 杨世裕, 呼天明, 韩祥艳, 杨培志. 垂穗披碱草EnWRKY26基因克隆及表达分析[J]. 草地学报, 2026, 34(6): 2032-2042.

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Cloning and Expression Analysis of the EnWRKY26 Gene in Elymus nutans Griseb

垂穗披碱草EnWRKY26基因克隆及表达分析

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