草地学报

›› 2004, Vol. 12 ›› Issue (4): 298-302.DOI: 10.11733/j.issn.1007-0435.2004.04.010

• 研究论文 • 上一篇    下一篇

鲁梅克斯k-1杂交酸模及野生酸模遗传多态性分析

张玉玲1, 鞠晓峰1, 杨青川2, 李柏林3, 杨茁萌1   

  1. 1. 新疆农业大学草业工程学院, 乌鲁木齐, 830052;
    2. 中国农业科学院畜牧研究所, 北京, 100094;
    3. 北京市农林科学院, 北京, 100089
  • 收稿日期:2003-05-22 修回日期:2004-10-21 出版日期:2004-11-15 发布日期:2004-11-15
  • 作者简介:张玉玲(1977- )女,内蒙古赤峰人,硕士,讲师,主要研究方向为牧草生物育种

Genetic Diversity of Rumex k-1(Rumex patientia×R.tianschanicus cv.Rumex k-1) and Wild Rumex spp.

ZHANG Yu-ling1, JU Xiao-feng1, YANG Qing-chuan2, LI Bo-lin3, YANG Zhuo-meng1   

  1. 1. Dept. of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang Uygur Autonomous Region 830052, China;
    2. Institute of Animal Science, Chinese Agricultural Acdemy, Beijing 100094 China;
    3. Beijing Agricultural and Forestry Acdemy, Beijing 100089 China
  • Received:2003-05-22 Revised:2004-10-21 Online:2004-11-15 Published:2004-11-15

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摘要： 利用RAPD技术对鲁梅克斯k-1杂交酸模的3个繁育世代和野生酸模进行遗传多态性分析,结果表明:3个世代之间不存在多态性位点差异,3个世代是稳定遗传的;不同种和不同地点的同一个野生种之间存在着多态性位点差异;在合格种的12个单株间具有明显的遗传多态性;采用混合取样可消除品种内的个体遗传差异性,较好地代表群体遗传特征;从0PA组选出7个能扩增出清晰且稳定带谱的引物,7个引物共检测出4个位点,DNA片段的大小在300～200bp之间.

关键词: 草原学, 鲁梅克斯k-1杂交酸模, 野生酸模, 遗传多样性, RAPD

Abstract: The genetic polymorphisms of three continued generations of Rumex k-1 (R. patientia×R.tianschanicus cv.Rumex k-1) and several wild Rumex spp. were analysed, using random amplified polymorphic DNA markers(RAPD). The result shows that there are no different polymorphic loci among the three successive generations of Rumex k-1, showing genetic stability, except for the 12 plants of the certified seed of Rumex k-1; while among the different populations of the same wild Rumex species growing in different sites, there appear abundant genetic polymorphisms loci. Seven primers selected from OPA twenty primers amplifies 45 RAPD fragments. The length of most of the amplified fragments range from 300 bp to 2500 bp.

Key words: Grassland science, Rumex k-1, Wild Rumex spp., Genetic polymorphism, Random amplified polymorphic DNA markers

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