草地学报

›› 2011, Vol. 19 ›› Issue (2): 340-345.DOI: 10.11733/j.issn.1007-0435.2011.02.027

• 研究论文 • 上一篇    下一篇

内蒙古地区草地螟白僵菌不同地区遗传多样性分析

刘爱萍1, 曹艺潇1, 徐林波1, 高书晶1, 吴晋华1, 谢丙仁2   

  1. 1. 中国农业科学院草原研究所, 呼和浩特, 010010;
    2. 内蒙古草原工作站, 呼和浩特, 010010
  • 收稿日期:2010-11-22 修回日期:2011-01-20 出版日期:2011-04-15 发布日期:2011-04-15
  • 作者简介:刘爱萍(1961- ),女,内蒙古呼和浩特人,研究员,主要从事草地害虫生物防治研究,E-mail:liuaiping806@sohu.com
  • 基金资助:
    公益性行业(农业)科研专项项目“草地害虫寄生性天敌昆虫利用新技术”(201103002)资助

Genetic Diversity of Beauveria bassiana on Loxostege sticticalis L.in Inner Mongolia

LIU Ai-ping1, CAO Yi-xiao1, XU Lin-bo1, GAO Shu-jin1, WU Jin-hua1, XIE Bing-ren2   

  1. 1. Grassland Research Institute of Chinese Academy of Agriculturl Sciences, Hohhot, Inner Mongolia 010010, China;
    2. Prairie Station of Inner Mongolia, Hohhot, Inner Mongolia 010010, China
  • Received:2010-11-22 Revised:2011-01-20 Online:2011-04-15 Published:2011-04-15

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摘要： 应用ISSR(简单重复序列间隔区)标记对采集自内蒙古3个地区的17株白僵菌(Beauveria Bassiana(Bals.)Vuill.)菌株进行遗传多样性分析,探讨了白僵菌在不同地区之间和地区内部的遗传多样性。结果表明:从21个引物中筛选出15个多态性高、稳定性好的ISSR引物,建立了ISSR的最佳反应体系。在ISSR试验中,15个ISSR引物共扩增出90条带,其中87条为多态性条带,群体多态位点百分率(PPL)为96.67%,Nei’s基因多样性指数(H)为0.4309,Shannon’s信息多样性指数(I)为0.6106。在群体间的遗传相似度聚类分析中,内蒙古中部和西部地区的遗传一致度最高为0.9897,兴安盟地区和鄂尔多斯市地区的遗传一致度最高为0.9621。

关键词: 白僵菌, ISSR, 遗传多样性, 亲缘关系

Abstract: Genetic diversity of 17 Beauveria bassiana strains collected from 3 different areas of Inner Mongolia was estimated using inter-simple sequence repeat(ISSR) markers.Fifteen high reproducibility and polymorphism ISSR primers were chosen from the twenty-one samples.The optimal reactive conditions were determined for using these primers.With 15 ISSR primers,a total of 90 fragments were amplified,in which in which 87 were polymorphic.The percentage of polymorphic loci(PPL) was 96.67%.Nei’s genetic diversity index(H) was 0.4309 and Shannon’s genetic diversity index(I) was 0.6106.The cluster analysis of the inter-population genetic differentiation showed that the highest genetic similarity of the western part compared with the central region was 0.9897 point and the highest genetic similarity of the eastern part with the western part was 0.9621 point.

Key words: Beauveria bassiana, ISSR, Genetic diversity, Sibship

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