火烧和施氮对内蒙古半干旱草原土壤微生物群落碳源利用潜力的影响

doi:10.11733/j.issn.1007-0435.2014.04.006

草地学报 ›› 2014, Vol. 22 ›› Issue (4): 713-721.DOI: 10.11733/j.issn.1007-0435.2014.04.006

火烧和施氮对内蒙古半干旱草原土壤微生物群落碳源利用潜力的影响

么中元^1,2, 张乃莉², 崔喜艳¹

1. 吉林农业大学生命科学学院, 长春吉林 130118;
2. 中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093

收稿日期:2013-11-06 修回日期:2014-01-22 出版日期:2014-08-15 发布日期:2014-08-04
通讯作者: 崔喜艳
作者简介:么中元（1989-），男，吉林长春人，硕士研究生，主要从事生物化学与分子生物学及植物生态学方面的研究，E-mail：495572155@qq.com
基金资助:
国家基金委面上项目（31270559）资助

Impacts of Fire and N Addition on Microbial C Utilization Potential in A Semi-arid Grassland of Inner Mongolia

YAO Zhong-yuan^1,2, ZHANG Nai-li², CUI Xi-yan¹

1. School of Sciences Jilin Agricultural University, Changchun, Jilin Province 130118, China;
2. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2013-11-06 Revised:2014-01-22 Online:2014-08-15 Published:2014-08-04

摘要/Abstract

摘要：

火烧是草原生态系统中最常见的一种自然现象，而氮素通常被认为是干旱、半干旱地区的主要限制因子。在中国北方草原生态系统相对干旱、长期处于氮素限制条件下，对火干扰和氮沉降非常敏感。通过在内蒙古多伦县半干旱草原地区进行火烧和施氮的野外控制试验，采用BIOLOG微孔板技术探讨火烧与施氮对土壤微生物量及其碳源利用的影响。结果表明：火烧和施氮处理显著降低生长季初期土壤微生物量；但是施氮显著促进了土壤细菌类群对多聚物、糖类、酚类和胺类碳源的利用，及真菌类群对胺类碳源的利用；而且火烧与施氮处理在影响土壤细菌碳源利用上表现出明显的交互效应，火烧削弱了施氮对细菌碳源利用的促进作用。火烧与施氮对生长季初期土壤微生物的这些影响，可能通过影响有效养分的释放而影响后期植物生长，但由于火烧与施氮处理在影响土壤微生物过程中表现出来的复杂性，这种对植物后期生长的影响可能存在较大的不确定性。

关键词: 火干扰, 氮沉降, 细菌, 真菌, 碳源利用

Abstract:

Fire is one of the most common natural phenomena in grassland ecosystem. Nitrogen is generally considered as the main limiting factor in arid and semiarid environment. It has been well documented that the grassland ecosystems of northern China are very sensitive to fire and N deposition due to the situation of water-deficiency and long term N-limitation. Here, a field experiment with annual burning and N addition was established in the semi-arid grassland of Inner Mongolia. Soil physicochemical properties, microbial biomass carbon (C) and N, and the C utilization potential of bacteria were measured using chloroform fumigation extraction method and BIOLOG microplate technique to test the responses of soil organic nutrients, microbial biomass and C utilization potential of bacteria to fire and N addition. The results showed that fire and N addition significantly decreased soil microbial biomass in the early growing season of plants. N addition largely modified the polymers carbohydrates, phenols and amines-carbon utilization of bacterial, and also promoted the amines-carbon utilization of fungi. In addition, fire and N addition had interaction effects on bacterial carbon utilization. Fire weakened the effects of N addition on improving bacterial carbon utilization. These results indicate that the effects of fire and N addition on soil microbial affect the plant growth of later stage by influencing the release of soil available nutrient. However, the interaction effects of fire and N addition on the plant growth of later stage have a greater uncertainty due to the complexity of both factors.

Key words: Fire, Nitrogen deposition, Bacteria, Fungi, Carbon source utilization

中图分类号:

么中元, 张乃莉, 崔喜艳. 火烧和施氮对内蒙古半干旱草原土壤微生物群落碳源利用潜力的影响[J]. 草地学报, 2014, 22(4): 713-721.

YAO Zhong-yuan, ZHANG Nai-li, CUI Xi-yan. Impacts of Fire and N Addition on Microbial C Utilization Potential in A Semi-arid Grassland of Inner Mongolia[J]. Acta Agrestia Sinica, 2014, 22(4): 713-721.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2014.04.006

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2014/V22/I4/713

参考文献

[1] 贺金生,王政权,方精云,等. 全球变化的地下生态学:问题与展望[J]. 科学通报,2004,49(13):1226-1233
[2] Zhang N, Wan S, Li L, et al. Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China [J]. Plant and Soil,2008,311(1/2):19-28
[3] Kandeler E, Palli S, Stemmer M, et al. Tillage changes microbial biomass and enzyme activities in particle-size fractions of a Haplic Chernozem[J]. Soil Biology and Biochemistry,1999,31(9):1253-1264
[4] Pickett S T. The ecology of natural disturbance and patch dynamics [M]. United Kingdom: Academic Press,1985:86
[5] Van Der Heijden M G A, Bardgett R D, Van Straalen N M. The unseen majority: Soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems [J]. Ecology Letters,2008,11(3):296-310
[6] 傅泽强,杨友孝,戴尔阜. 内蒙古干草原火动态及火险气候区划研究[J]. 中国农业资源与区划,2001,22(6):18-22
[7] 原烨,张钊,赵宁,等. 火烧和刈割耙除对草原土壤理化性质的影响[J]. 草业科学,2011,28(10):1770-1776
[8] 周瑞莲,张普金. 高寒山区火烧土壤对其养分含量和酶活性的影响及灰色关联分析[J]. 土壤学报,1997,34(1):89-96
[9] Bauhus J, Khanna P K, Raison R J. The effect of fire on carbon and nitrogen mineralization and nitrification in an Australian forest soil [J]. Soil Research,1993,31(5):621-639
[10] Pardini G, Gispert M, Dunjó G. Relative influence of wildfire on soil properties and erosion processes in different Mediterranean environments in NE Spain [J]. Science of the Total Environment,2004,328(1):237-246
[11] 白爱芹,傅伯杰,曲来叶,等. 大兴安岭火烧迹地恢复初期土壤微生物群落特征[J]. 生态学报,2012,32(15):4762-4771
[12] Bai Y, Wu J, Clark C M, et al. Tradeoffs and thresholds in the effects of nitrogen addition on biodiversity and ecosystem functioning: Evidence from inner Mongolia Grasslands[J]. Global Change Biology,2010,16(1):358-372
[13] Bassin S, Volk M, Suter M, et al. Nitrogen deposition but not ozone affects productivity and community composition of subalpine grassland after 3 yr of treatment [J]. New Phytologist,2007,175(3):523-534
[14] Clark C M, Tilman D. Loss of plant species after chronic low-level nitrogen deposition to prairie grasslands[J]. Nature,2008,451(7179):712-715
[15] 孙凤霞,张伟华,徐明岗. 长期施肥对红壤微生物生物量碳氮和微生物碳源利用的影响[J]. 应用生态学报,2010,21(11):2792-2798
[16] Treseder K K. Nitrogen additions and microbial biomass: A meta-analysis of ecosystem studies [J]. Ecology Letters,2008,11(10):1111-1120
[17] Söderström B, Bååth E, Lundgren B. Decrease in soil microbial activity and biomasses owing to nitrogen amendments[J]. Canadian Journal of Microbiology,1983,29(11):1500-1506
[18] Guo J H, Liu X J, Zhang Y, et al. Significant acidification in major Chinese croplands [J]. Science,2010,327(5968):1008-1010
[19] Bardgett R D, Lovell R D, Hobbs P J, et al. Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands [J]. Soil Biology and Biochemistry,1999,31(7):1021-1030
[20] 刘先华,李凌浩. 内蒙古锡林河流域植被多样性特点及其与气候因子的关系[J]. 植物生态学报,1998,22(5):466-472
[21] Schmidt S K, Lipson D A. Microbial growth under the snow: implications for nutrient and allelochemical availability in temperate soils [J]. Plant and Soil,2004,259(1/2):1-7
[22] 赵艳,张晓波. 返青前后草地早熟禾草坪根际微生物区系动态[J]. 生态环境,2007,16(6):1733-1736
[23] Brookes P C, Landman A, Pruden G, et al. Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil [J]. Soil Biology and Biochemistry,1985,17(6):837-842
[24] Vance E D, Brookes P C, Jenkinson D S. Microbial biomass measurements in forest soils: The use of the chloroform fumigation-incubation method in strongly acid soils [J]. Soil Biology and Biochemistry,1987,19(6):697-702
[25] Joergensen R G, Mueller T. The fumigation-extraction method to estimate soil microbial biomass: Calibration of the K_ec value [J]. Soil Biology and Biochemistry,1996,28(1):33-37
[26] Classen A T, Boyle S I, Haskins K E, et al. Community‐level physiological profiles of bacteria and fungi: plate type and incubation temperature influences on contrasting soils[J]. FEMS Microbiology Ecology,2003,44(3):319-328
[27] Liu W, Xu W, Hong J, et al. Interannual variability of soil microbial biomass and respiration in responses to topography, annual burning and N addition in a semiarid temperate steppe[J]. Geoderma,2010,158(3):259-267
[28] Frey S D, Knorr M, Parrent J L, et al. Chronic nitrogen enrichment affects the structure and function of the soil microbial community in temperate hardwood and pine forests [J]. Forest Ecology and Management,2004,196(1):159-171
[29] DeForest J L, Zak D R, Pregitzer K S, et al. Atmospheric nitrate deposition and the microbial degradation of cellobiose and vanillin in a northern hardwood forest [J]. Soil Biology and Biochemistry,2004,36(6):965-971
[30] Su J, Li X, Li X, et al. Effects of additional N on herbaceous species of desertified steppe in arid regions of China: A four-year field study [J]. Ecological Research,2013,28(1):21-28
[31] Yang H, Li Y, Wu M, et al. Plant community responses to nitrogen addition and increased precipitation: The importance of water availability and species traits [J]. Global Change Biology,2011,17(9):2936-2944
[32] Anthony G O, Donnell, Melanie Seasman, et al. Plants and fertilizers as drivers of changes in microbial community structure and function in soils [J]. Plant and Soil,2001,232(1):135-145
[33] Rubio G, Zhu J, Lynch J P. A critical test of the two prevailing theories of plant response to nutrient availability [J]. American Journal of Botany,2003,90(1):143-152
[34] Greenberg C H, Tomcho A L, Lanham J, et al. Short-term effects of fire and other fuel reduction treatments on breeding birds in a southern Appalachian Upland Hardwood Forest [J]. The Journal of Wildlife Management,2007,71(6):1906-1916
[35] Stephens S L, Moghaddas J J. Experimental fuel treatment impacts on forest structure, potential fire behavior, and predicted tree mortality in a California mixed conifer forest[J]. Forest Ecology and Management,2005,215(1):21-36
[36] 吴建峰,林先贵. 土壤微生物在促进植物生长方面的作用[J]. 土壤,2003,35(1):18-21
[37] Stevens C J, Dise N B, Mountford J O, et al. Impact of nitrogen deposition on the species richness of grasslands [J]. Science,2004,303(5665):1876-1879

火烧和施氮对内蒙古半干旱草原土壤微生物群落碳源利用潜力的影响

Impacts of Fire and N Addition on Microbial C Utilization Potential in A Semi-arid Grassland of Inner Mongolia

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄彩霞, 芦光新, 李欣, 范月君, 周华坤, 王英成, 王志慧, 姚世庭. 产漆酶真菌对高寒草甸土壤有机质矿化特性的研究[J]. 草地学报, 2021, 29(S1): 173-178.
[2]	石国玺, 王芳萍, 马丽, 张中华, 王弋博, 汪之波, 姚步青, 周华坤. 长期、短期增温对高寒草甸AM真菌群落结构的影响[J]. 草地学报, 2021, 29(S1): 179-189.
[3]	王谭国艳, 马志远, 李沛洋, 郭俊宏, 张嘉懿, 蒋胜竞. 短期增温对青藏高原高寒草甸不同植物根际丛枝菌根真菌的影响[J]. 草地学报, 2021, 29(9): 1959-1966.
[4]	魏勇, 高嵩涓, 曹卫东, 段廷玉. 绿肥影响农田土传病害的研究进展[J]. 草地学报, 2021, 29(8): 1605-1614.
[5]	连鹤娜, 陈泰祥, 陈振江, 魏学凯, 李春杰. 内生真菌醉马草作为机场驱鸟草的潜力分析[J]. 草地学报, 2021, 29(8): 1649-1657.
[6]	唐丽辉, 刘奕伶, 赵文星, 张云昊, 吴可欣, 郭梓豫, 莫重辉, 赵宝玉, 路浩. 箭叶橐吾内生真菌分离鉴定及组织分布特性研究[J]. 草地学报, 2021, 29(8): 1658-1665.
[7]	郑敏娜, 梁秀芝, 韩志顺, 康佳惠, 陈燕妮. 不同改良措施对盐碱土土壤细菌群落多样性的影响[J]. 草地学报, 2021, 29(6): 1200-1209.
[8]	孙雷雷, 赵桂琴, 柴继宽, 刘富渊, 聂秀美, 王军. 贮藏方式和贮藏时间对苜蓿干草霉菌数量和种类的影响[J]. 草地学报, 2021, 29(5): 884-893.
[9]	王新宇, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 郭刚. 雁北地区不同品种玉米全株青贮发酵品质及细菌群落结构分析[J]. 草地学报, 2021, 29(5): 1087-1093.
[10]	潘越, 周冀琼, 郭川, 杨喆, 刘琳, 闫艳红, 赵艳兰, 刘珊, 孙飞达. 丛枝菌根真菌与根瘤菌对3种豆禾混播植物种间互作的影响[J]. 草地学报, 2021, 29(4): 644-654.
[11]	陆永祥, 严显明, 周朝相, 朱会先, 苟文龙, 游明鸿, 李达旭, 陈仕勇, 李平, 白史且. 不同添加剂对高水分全株玉米青贮饲料发酵品质和细菌群落的影响[J]. 草地学报, 2021, 29(4): 842-847.
[12]	任璐, 刘晓峰, 周建波, 殷辉, 张宝俊, 赵晓军. 醉鱼草内生细菌ZJ1的抑菌作用及其脂肽类抑菌代谢产物鉴定[J]. 草地学报, 2021, 29(3): 434-442.
[13]	吴建波, 王小丹. 藏北高寒草原土壤酶活性对氮添加的响应及其影响因素[J]. 草地学报, 2021, 29(3): 555-562.
[14]	苏贝贝, 张英, 道日娜. 4种豆科栽培牧草根际土壤细菌群落分布特征研究[J]. 草地学报, 2021, 29(2): 250-258.
[15]	王丽学, 韩静, 陈龙宾, 余新越, 刘景喜, 马毅, 霍文娟. 不同乳酸菌组合对苜蓿青贮细菌群落结构的影响[J]. 草地学报, 2021, 29(2): 388-395.