镉在草地早熟禾中的分布和化学形态研究

doi:10.11733/j.issn.1007-0435.2023.01.008

草地学报 ›› 2023, Vol. 31 ›› Issue (1): 73-80.DOI: 10.11733/j.issn.1007-0435.2023.01.008

镉在草地早熟禾中的分布和化学形态研究

崔婷, 王勇, 牛奎举, 董文科, 张然, 马晖玲

甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

收稿日期:2022-06-24 修回日期:2022-08-14 出版日期:2023-01-15 发布日期:2023-02-07
通讯作者: 马晖玲, E-mail:mahl@gsau.edu.cn
作者简介:崔婷(1996-)，女，甘肃会宁人，硕士研究生，主要从事重金属污染的植物修复研究，E-mail:3262605496@qq.com
基金资助:
甘肃省优秀研究生“创新之星”项目(2021CXZX-385)资助

Study on Distribution and Chemical Forms of Cd in Poa pratensis

CUI Ting, WANG Yong, NIU Kui-ju, DONG Wen-ke, ZHANG Ran, MA Hui-ling

College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Center for Grazing land Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2022-06-24 Revised:2022-08-14 Online:2023-01-15 Published:2023-02-07

摘要/Abstract

摘要： 为探究草地早熟禾(Poa pratensis L.)对重金属镉(Cadmium，Cd)的分配和耐受机制，以草地早熟禾耐Cd品种'午夜’('Midnight’，M)和Cd敏感品种'橄榄球2号’('Rugby II’，R)为试验材料，在水培条件下施加600 μmol·L^-1Cd胁迫，研究Cd在草地早熟禾中的组织分布、亚细胞分布及化学形态。结果表明，与R品种相比，Cd胁迫下M品种叶片失绿较少，生长抑制较小；表皮+叶肉、维管束及根表皮+皮层中的Cd含量显著低于R品种；另外，根系可溶部分Cd含量高于R品种。进一步分析发现，R品种含有更多毒性较高的无机态Cd及果胶和蛋白质结合态Cd，而M品种水溶性有机酸结合的Cd在根表皮+皮层、中柱等组织中含量较多，草酸盐结合的Cd在根系中柱含量显著高于R品种。因此，M品种通过减少Cd吸收、增加液泡区隔化、增加有机酸盐结合的Cd并降低Cd向地上的长距离运输来增强对Cd的抗性。

关键词: Cd胁迫, 草地早熟禾, 亚细胞分布, 化学形态

Abstract: To investigate the Cd distribution and tolerance mechanisms in Kentucky bluegrass，the Cd-tolerant and Cd-sensitive cultivars named Midnight (M) and Rugby (R) were used as the experimental materials. The distribution and chemical forms of Cd in tissue and subcellular at a hydroponic culture condition were studied under 600 μmol·L^-1Cd stress. The results showed that M genotype had less leaf chlorosis and growth inhibition under Cd stress compared with R genotype. The Cd concentrations in epidermis + mesophyll,vascular bundle,and rhizodermis + cortex of M genotype were significantly lower than that of R genotype. In addition,the soluble Cd concentrations in roots of M genotype were higher than that of R genotype. The inorganic Cd,pectinate and protein bound Cd in R genotype were higher than those in M genotype,while the M genotype had higher concentration of water-soluble organic acid bound Cd in rhizodermis + cortex and stele,and oxalate-bound Cd in stele. Therefore,the M genotype increased Cd tolerance by decreasing the absorption of Cd,increasing the vacuolar compartmentation,organic acid bound Cd,and decreasing the long-distance translocation of Cd from roots to shoots.

Key words: Cd stress, Kentucky bluegrass, Subcellular distribution, Chemical forms

中图分类号:

S812.5

崔婷, 王勇, 牛奎举, 董文科, 张然, 马晖玲. 镉在草地早熟禾中的分布和化学形态研究[J]. 草地学报, 2023, 31(1): 73-80.

CUI Ting, WANG Yong, NIU Kui-ju, DONG Wen-ke, ZHANG Ran, MA Hui-ling. Study on Distribution and Chemical Forms of Cd in Poa pratensis[J]. Acta Agrestia Sinica, 2023, 31(1): 73-80.

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镉在草地早熟禾中的分布和化学形态研究

Study on Distribution and Chemical Forms of Cd in Poa pratensis

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