1株高寒草地土壤真菌的鉴定及产纤维素酶特性的研究

doi:10.11733/j.issn.1007-0435.2018.02.033

草地学报 ›› 2018, Vol. 26 ›› Issue (2): 505-511.DOI: 10.11733/j.issn.1007-0435.2018.02.033

1株高寒草地土壤真菌的鉴定及产纤维素酶特性的研究

李欣¹, 芦光新¹, 王军邦², 姚世庭¹, 党宁¹, 周华坤³, 张英¹, 谢永丽¹

1. 青海大学, 青海西宁 810016;
2. 中国科学院地理科学与资源研究所生态系统网络观测与摸拟重点实验室, 北京 100101;
3. 中国科学院西北高原生物研究所, 青海省寒区恢复生态学重点实验室, 青海西宁 810008

收稿日期:2017-04-04 修回日期:2018-03-21 出版日期:2018-04-15 发布日期:2018-07-05
通讯作者: 芦光新, 王军邦
作者简介:李欣(1993-),女,青海大通人,硕士研究生,E-mail:907434358@qq.com
基金资助:
国家自然科学基金项目31460152；41261064；青海省创新平台建设专项（2017-ZJ-Y20）资助

Identification of a Soil Fungus Strain Isolated from an Alpine Grassland and Characteristics of Cellulase Production

LI Xin¹, LU Guang-xin¹, WANG Jun-bang², YAO Shi-ting¹, DANG Ning¹, ZHOU Hua-kun³, ZHANG Ying¹, XIE Yong-li¹

1. Qinghai University, Xining, Qinghai Province 810016, China;
2. Key Lab of Ecosystem Network Observation and Modeling of CAS Synthesis Research Center of Chinese Ecosystem Research Network, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
3. Key Laboratory of Cold Regions Restoration Ecology, Qinghai Province, Northwest Plateau Institute of Biology, Xining, Qinghai Province 810008, China

Received:2017-04-04 Revised:2018-03-21 Online:2018-04-15 Published:2018-07-05

摘要/Abstract

摘要：

为研究1株高寒草地土壤真菌的产纤维素酶特性，采用微生物培养法和液体摇瓶发酵法，对供试菌株的培养特性和产酶特性进行了研究。结果表明：经ITS-rDNA分子鉴定，供试菌株初步确定为Thielavia hyalocarpa，供试菌株的最适生长温度为25℃；对不同的碳、氮源利用能力不同，在供试的5种碳源和氮源中，葡萄糖和蛋白胨分别为最适碳源和氮源，对5种碳源的利用表现为：葡糖糖 > 糊精、麦芽糖和淀粉 > 蔗糖菌株， H对5种氮源的利用表现为：蛋白质 > 硝酸钠 > 尿素 > 硫酸胺 > 磷酸胺；菌株H产的纤维素酶在pH 5.5和50℃下有较高的催化活性，产的木聚糖酶在pH为5和55℃温度下有较高的催化活性。

关键词: 高寒草地, 纤维素酶, 木聚糖酶, 培养特性

Abstract:

To study the celluse-producing characteristics of a soil fungus isolated from an alpine meadow, the culture characteristics and enzyme production characteristics of the tested strains were studied by using the traditional microorganism culture method and liquid shake flask fermentation method. The results showed that the strain was identified as Thielavia hyalocarpa by ITS-rDNA molecular identification. The optimum growth temperature was 25℃. Five carbon sources and nitrogen sources were tested. Among the nitrogen sources, glucose and peptone were the most suitable carbon sources and nitrogen sources, respectively. The utilization of five carbon sources was as follows:glucosamine > dextrin, maltose and starch > sucrose. The performance of strain H on five nitrogen sources was as follows:protein > sodium nitrate > urea > amine sulfate > ammonium phosphate; cellulase produced by strain H has high catalytic activity at pH 5.5 and 50℃, and the pH value of produced xylanase is 5, it had a high catalytic activity at the temperature 55℃.

Key words: Alpine meadows, Cellulase, Xylanase, Biological characteristics

中图分类号:

S154

李欣, 芦光新, 王军邦, 姚世庭, 党宁, 周华坤, 张英, 谢永丽. 1株高寒草地土壤真菌的鉴定及产纤维素酶特性的研究[J]. 草地学报, 2018, 26(2): 505-511.

LI Xin, LU Guang-xin, WANG Jun-bang, YAO Shi-ting, DANG Ning, ZHOU Hua-kun, ZHANG Ying, XIE Yong-li. Identification of a Soil Fungus Strain Isolated from an Alpine Grassland and Characteristics of Cellulase Production[J]. Acta Agrestia Sinica, 2018, 26(2): 505-511.

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1株高寒草地土壤真菌的鉴定及产纤维素酶特性的研究

Identification of a Soil Fungus Strain Isolated from an Alpine Grassland and Characteristics of Cellulase Production

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