GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控

doi:10.11733/j.issn.1007-0435.2020.06.005

草地学报 ›› 2020, Vol. 28 ›› Issue (6): 1527-1534.DOI: 10.11733/j.issn.1007-0435.2020.06.005

GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控

赵利清^1,2, 彭向永³, 刘俊祥¹, 毛金梅⁴, 孙振元¹

1. 国家林业和草原局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091;
2. 国家开放大学, 北京 100039;
3. 曲阜师范大学生命科学学院, 山东曲阜 273165;
4. 新疆林业科学院经济林研究所, 新疆乌鲁木齐 830063

收稿日期:2020-07-14 修回日期:2020-08-19 出版日期:2020-12-15 发布日期:2020-12-02
通讯作者: 孙振元
作者简介:赵利清(1976-),女,内蒙古呼和浩特人,博士研究生,副教授,主要从事园林植物的分子育种研究,E-mail:195432830@qq.com
基金资助:
中央级公益性科研院所基本科研业务费专项资金（CAFYBB2018ZB002）资助

Effects of Glutathione(GSH)on Antioxidant Defense System of Lolium Perenne Seedlings under Lead Stress

ZHAO Li-qing^1,2, PENG Xiang-yong³, LIU Jun-xiang¹, MAO Jin-mei⁴, SUN Zhen-yuan¹

1. Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Foresstry, Beijing 100091, China;
2. The Open University of China, Beijing 100039, China;
3. Qufu Normal University, Qufu, Shandong Province 273165, China;
4. Economic Forest Research Institute Xinjiang Academy of Forestry, Urumqi, Xinjiang 830063, China

Received:2020-07-14 Revised:2020-08-19 Online:2020-12-15 Published:2020-12-02

摘要/Abstract

摘要： 本试验以12周龄的多年生黑麦草（Lolium perenne ‘cuttle’）幼苗为试验材料，用铅（Lead，Pb），Pb+还原型谷胱甘肽（Glutathione，GSH）和Pb+丁硫氨酸-亚砜亚胺（L-Buthionine-sulfoximine，BSO）处理1周，研究GSH对Pb胁迫下多年生黑麦草抗氧化系统的调控机理，寻求缓解植物Pb胁迫的有效措施。结果表明：Pb胁迫下，外源GSH能够显著提高多年生黑麦草Pb的吸收和转运，增加抗氧化酶超氧化物歧化酶（Superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GPX）和谷胱甘肽还原酶（Glutathione reductase，GR）活性，显著增加抗氧化剂GSH含量及GSH/氧化型谷胱甘肽（Oxidized glutathione，GSSG）比率，提高了植物的还原力。外源GSH也能够显著降低活性氧超氧阴离子（Superoxide anion，O₂^·-）和过氧化氢（Hydrogen peroxide，H₂O₂）的产生量、膜的相对透性（Relative electric conductivity，EC）和丙二醛（Malondialdehyde，MDA）含量，保持了细胞的稳定性，减轻了膜脂的过氧化程度。外源BSO作用基本上与GSH相反。相关分析表明：GSH可通过提高SOD和GPX等抗氧化酶的活性，促进GSSG和GSH的相互转化，降低活性氧产生量，提高植物抵抗逆境胁迫的能力。

关键词: 多年生黑麦草, 谷胱甘肽, 抗氧化系统, 铅

Abstract: In this study,Lolium perenne seedlings (12 weeks old) were exposed to Pb,Pb+GSH and Pb+BSO treatment for one week to investigate effects of glutathione on antioxidant defense system of leaf and root. The results showed that Pb+GSH treatment increased Pb absorption and transport. It also increased antioxidase activity such as superoxide dismutase(SOD),glutathione peroxidase(GPX) and glutathione reduction(GR),antioxidant GSH content and GSH/GSSG ratio. It enhanced Seedlings' reducing power. On the contrary,the content of ROS(O₂^·- and H₂O₂),malondialdehyde content (MDA) and relative electric conductivity(EC) declined under Pb+GSH treatment. Pb+BSO treatment had almost opposite effects to that of Pb+GSH treatment. The correlation analysis indicated that GSH could reduce reactive oxygen species production and alleviate oxidative stress by increasing antioxidase activity and promoting the mutual transformation of GSSG and GSH.

Key words: Lolium perenne, Glutathione, Antioxidant defense system, Lead

中图分类号:

赵利清, 彭向永, 刘俊祥, 毛金梅, 孙振元. GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控[J]. 草地学报, 2020, 28(6): 1527-1534.

ZHAO Li-qing, PENG Xiang-yong, LIU Jun-xiang, MAO Jin-mei, SUN Zhen-yuan. Effects of Glutathione(GSH)on Antioxidant Defense System of Lolium Perenne Seedlings under Lead Stress[J]. Acta Agrestia Sinica, 2020, 28(6): 1527-1534.

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参考文献

[1] 原海燕,郭智,佟海英,等. Pb胁迫下外源GSH对马蔺体内Pb积累和非蛋白巯基化合物含量的影响[J]. 水土保持学报,2013,27(4):212-216
[2] Wahsha M,Bini C,Fontana S,et al. Toxicity assessment of contaminated soils from a mining area in Northeast Italy by using lipid peroxidation assay[J]. Journal of Geochemical Exploration,2012(113):112-117
[3] Hasanuzzaman M,Nahar K,Rahman A,et al. Exogenous glutathione attenuates lead-induced oxidative stress in wheat by improving antioxidant defense and physiological mechanisms[J]. Journal of Plant Interactions,2018,13(1):203-212
[4] 王日明,王志强,向佐湘. 外源γ-氨基丁酸对高温胁迫下黑麦草抗氧化防御系统及激素代谢的影响[J]. 草业科学,2019,36(1):111-122
[5] 华春,王仁雷,刘友良. 外源GSH对盐胁迫下水稻叶绿体活性氧清除系统的影响[J]. 植物生理与分子生物学学报,2003,29(5):415-420
[6] 闫慧芳,毛培胜,夏方山. 植物抗氧化剂谷胱甘肽研究进展[J]. 草地学报,2013,21(3):428-434
[7] Anjum N A,Ahmad I,Mohmood I,et al. Modulation of glutathione and its related enzymes in plants' responses to toxic metals and metalloids-A review[J]. Environmental and Experimental Botany,2012(75):307-324
[8] 赵娟,施国新,徐勤松,等. 外源谷胱甘肽(GSH)对水鳖Zn²⁺毒害的缓解作用[J]. 热带亚热带植物学报,2006(3):213-217
[9] Griffith O W,Meister A. Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine)[J]. Journal of Biological Chemistry,1979,254(16):7558-7560
[10] Mariana E,Dario F,Elizabeth A,et al. Glutathione,a key compound for As accumulation and tolerance in soybean plants treated with AsV and AsIII[J]. Environmental and Experimental Botany,2019(162):272-282
[11] 刘俊祥,孙振元,勾萍,等. 镉胁迫下多年生黑麦草的光合生理响应[J]. 草业学报,2012,21(3):191-197
[12] 周艳,刘慧英,邓嘉欣,等. GSH/GSSG对盐胁迫下番茄幼苗谷胱甘肽化修饰和抗氧化系统的影响[J/OL]. 分子植物育种,2020-05-28/2020-07-07
[13] Doke N,Ohashi Y. Involvement of an O₂^.- generating system in the induction of necrotic lesions on tobacco leaves infected with tobacco mosaic virus[J]. Physiological and Molecular Plant Pathology,1988,32(1):163-175
[14] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000:194-197,260-261
[15] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of microgram quantities of protein utilizing the principle of protein dye binding[J]. Biochemistry,1976,72(1-2):248-254
[16] 陈晶晶,王英哲,金艳,等. 铅胁迫对14个紫花苜蓿杂交组合生理生化指标的影响[J]. 草地学报,2018,26(5):1277-1282
[17] Wang H H,Shan X Q,Wen B,et al. Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response[J]. Environmental and Experimental Botany,2007,61(3):246-253
[18] Pourrut B,Perchet G,Silvestre J, et al. Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots[J]. Journal of Plant Physiology,2008,165(6):571-579
[19] 付晴晴,谭雅中,翟衡,等. NaCl胁迫对耐盐性不同葡萄株系叶片活性氧代谢及清除系统的影响[J]. 园艺学报,2018,45(1):30-40
[20] 毛雪飞,杨洁. 锌镉胁迫下4种农田杂草生理生化特性及对重金属的累积特征[J]. 西南林业大学学报(自然科学),2019,39(6):9-18
[21] 刘霄霏,李惠英,陈良,等. 外源硒对镉胁迫下黑麦草生长和生理的影响[J]. 草地学报,2020,28(1):72-79
[22] 丁继军,刘柿良,潘远智,等. 外源AsA、GSH对Cd胁迫下石竹幼苗生长的影响[J]. 应用生态学报,2014,25(2):419-426
[23] 段喜华,唐中华,郭晓瑞. 植物谷胱甘肽的生物合成及其生物学功能[J]. 植物研究,2010,30(1):98-105
[24] Yuan H Y,Guo Z,Liu Q Q,et al. Exogenous glutathione increased lead uptake and accumulation in Iris lactea var. chinensis exposed to excess lead[J]. International Journal of Phytoremediation,2018,20(11):1136-1143
[25] Nakamura S,Wongkaew A,Nakai Y,et al. Foliar-applied glutathione activates zinc transport from roots to shoots in oilseed rape[J]. Plant Science,2018(283):424-434
[26] John L F,Michael W P,Ken N,et al. Increased glutathione biosynthesis plays a role in nickel tolerance in thlaspi nickel hyperaccumulators[J]. The Plant Cell,2004,16(8):2176-2191
[27] Nakamura S I,Suzui N,Ito-Tanabata S,et al. Application of glutathione and dithiothreitol to oil seed rape (Brassica napus L.) roots affects cadmium distribution in roots and inhibits Cd translocation to shoots[J]. Soil Science and Plant Nutrition,2016,62(4):379-385
[28] Requejo R,Tena M. Influence of glutathione chemical effectors in the response of maize to arsenic exposure[J]. Journal of Plant Physiology,2012,169(7):649-656
[29] Khan M,Daud M K,asharat A,et al. Alleviation of lead-induced physiological,metabolic and ultramorphological changes in leaves of upland cotton through glutathione[J]. Environmental Science and Pollution Research,2016,23(9):8431-8440
[30] 丁继军,潘远智,李丽,等. 外源谷胱甘肽对石竹幼苗镉毒害的缓解效应[J]. 植物生态学报,2013,37(10):950-960
[31] 李西,吴亚娇,孙凌霞. 铅胁迫对三种暖季型草坪草生长和生理特性的影响[J]. 草业学报,2014,23(4):171-180
[32] Mittler R. Oxidative stress,antioxidants and stress tolerance[J]. Trends in Plant Science,2002,7(9):405-410
[33] Margis R,Dunand C,Teixeira F K,et al. Glutathione peroxidase family-an evolutionary overview[J]. Febs Journal,2008,275(15):3959-3970
[34] 刘传平,郑爱珍,田娜,等. 外源GSH对青菜和大白菜镉毒害的缓解作用[J]. 南京农业大学学报,2004,27(4):26-30
[35] 丁顺华,陈珊,卢从明. 植物叶绿体谷胱甘肽还原酶的功能研究进展[J]. 植物生理学报,2016,52(11):1703-1709

GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控

Effects of Glutathione(GSH)on Antioxidant Defense System of Lolium Perenne Seedlings under Lead Stress

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