草地学报

草地学报 ›› 2015, Vol. 23 ›› Issue (5): 1114-1118.DOI: 10.11733/j.issn.1007-0435.2015.05.033

• 研究专报 • 上一篇    下一篇

辐射白刺花遗传多样性的ISSR和RAPD比较分析

李凤涛1, 赵丽丽1, 王普昶2, 陈超1, 吴小丽1   

  1. 1. 贵州大学动物科学学院草业科学系, 贵州 贵阳 550025;
    2. 贵州省草业研究所, 贵州 贵阳 550025
  • 收稿日期:2014-08-04 修回日期:2015-01-22 出版日期:2015-10-15 发布日期:2015-12-01
  • 通讯作者: 赵丽丽
  • 作者简介:李凤涛(1989-),女,贵州贵阳人,硕士,主要从事草地生态与管理,E-mail:lft23520@163.com
  • 基金资助:

    贵州省科教青年英才培育工程项目[黔省专合字(2012)142号];国家自然科学基金(31260572);贵州大学引进人才科研项目[贵大人基合字(2010)036号];贵州大学教改项目(JG2013067)资助

Comparison of Genetic Diversity of Irradiated Sophora viciifolia Using ISSR and RAPD Markers

LI Feng-tao1, ZHAO Li-li1, WANG Pu-chang2, CHEN Chao1, WU Xiao-li1   

  1. 1. College of Animal Sciences, Guizhou University, Guiyang, Guizhou Province 550025, China;
    2. Guizhou Practaculture Research Institute, Guiyang, Guizhou Province 550006, China
  • Received:2014-08-04 Revised:2015-01-22 Online:2015-10-15 Published:2015-12-01

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摘要：

采用ISSR和RAPD标记对8个60Co-γ辐射梯度诱变白刺花(Sophora viciifolia)进行遗传多样性分析。结果表明:20条ISSR和6条RAPD引物的PPL多态率分别为51.37%和21.03%,ISSR的Nei's指数和平均Shannon指数均大于RAPD;ISSR的标记指数(1.28)是RAPD(0.20)的6.4倍,表明ISSR具有较高的标记效率。Mantel检测表明,ISSR+RAPD标记与ISSR标记的结果显著相关(P<0.05)。综合分析表明白刺花用ISSR标记可检测出更高的多态性,更能准确反映白刺花材料间的遗传关系。

关键词: 白刺花, 辐射诱变, ISSR, RAPD, 遗传多样性

Abstract:

The RAPA and ISSR markers were used in analyzing the genetic diversity of mutagenic Sophora viciifolia Franch with eight different 60Co-γ-rays gradients. The results showed that the percentage of polymorphic bands of 20 ISSR primers and 6 RAPD primers were 51.27% and 21.03%, respectively. The Nei's gene heterozygosity and Shannon's information index of ISSR were greater than RAPD. For 8 materials, ISSRs got a MI value(1.28) that was 6.4 times higher than that of RAPDs(0.20), indicating that ISSRs had a higher MI efficiency. The Mantel test revealed that the correlation was significant between ISSR+RAPDs and ISSRs(r=0.9791, P<0.05). Comprehensive research showed that ISSRs could detect the higher polymorphism of Sophora viciifolia, and more accurately reflect the genetic relationship among Sophora viciifolia.

Key words: Sophora viciifolia, Radiant mutagenesis, ISSR, RAPD, Genetic diversity

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