Mycosystema

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Domestication and cultivation of a wild Pholiota limonella strain

GUO Zhihao, GENG Binbin, ZHANG Guoli, ZHANG Yinghao, TIAN Xuemei

Mycosystema, 2024, 43(12): 240140. DOI: 10.13346/j.mycosystema.240140

水平 Level	因素 Factors
水平 Level	玉米芯 Corn cob (%)	香菇菌渣 Lentinus edodes residue (%)	贝壳粉 Shell powder (%)	麸皮 Wheat bran (%)
1	0	0	0	0
2	15	30	2	10
3	30	50	4	20

Table 1 Design of L₉(3⁴) orthogonal experiment of Pholiota limonella cultivation material screening

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Fig. 1 Colonial morphology of plate culture (A) and liquid culture (B) of Pholiota limonella.
Fig. 2 A comparison of the number of fruiting bodies per cluster in the first two crops of the domestic cultivation of wild Pholiota limonella. Different capital letters mean extremely significant difference at the 0.01 level. Different small letters mean significant difference at 0.05 level. The same below.
Table 2 A comparison of parameters of cap and stipe of fruiting body in formulation screening test of domestic cultivation substrate of the wild Pholiota limonella
Fig. 3 Cultivated fruiting bodies of Pholiota limonella. A: Top surface of pilei; B: Underside of pilei.
Table 3 Agronomic traits of domestication cultivation of Pholiota limonella
Fig. 4 Fruiting bodies of Pholiota limonella of different fruiting models. A: Porous fruiting mode in perforated bag; B: Top fruiting mode of bag-cultivation; C: Fruiting bodies of porous fruiting mode; D: Fruiting bodies of top fruiting mode.
Fig. 5 A comparison of biological efficiency of different fruiting patterns of the cultivated Pholiota limonella.
Table 4 Orthogonal experiment variance analysis of formula optimization of Pholiota limonella cultivation substrate containing Lentinula edodes residue
Table 5 Biological efficiency and range analysis of orthogonal experiment on formula optimization of cultivation substract containing Lentinula edodes residue
Table 6 Results of amino acid analysis of fruiting bodies of cultivated Pholiota limonella
Table 7 Percentage of essential amino acids in total amino acids of Pholiota limonella fruiting bodies
Table 8 Comparison of RAA, RC and SRC of the essential amino acids of Pholiota limonella

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