The Antioxidative Function Changes in Embryonic Mitochondria of Elymus sibiricus Seeds of Different Storage Years
TANG Jia, MAO Chun-li, HUANG Qi, TANG Jun, SONG Yu-mei, SUN Ming, MAO Pei-sheng
College of Grassland Science and Technology, China Agricultural University, Key Laboratory of Pratacultural Science, Beijing Municipality, Beijing 100193, China
Abstract:Deterioration of internal physiological functions often occurs in seeds during natural storage. As the main site of reactive oxide species (ROS) production,mitochondria are the first destroyed organelles during the process of seed aging. To investigate the effect of physiological function in embryonic mitochondria of Siberian wildrye (Elymus sibiricus L.) seeds during the different storage duration,seed samples were evaluated after stored at room temperature for 0~4 years. The results showed that the germination potential,germination percentage and seedling fresh weight gradually decreased,and the number of dead seeds increased with the prolongation of storage duration. The activities of embryonic mitochondria malate dehydrogenase (MDH),glutathione reductase (GR),monodehydroascorbate reductase (MDHAR) gradually decreased,but the activity of superoxide dismutase (SOD) in dead seeds increased significantly. In addition,the production rate of mitochondrial O2·- increased,while mitochondrial H2O2 content showed a gradually decreasing trend. It indicated that mitochondrial O2·- accumulation was closely related to the cell oxidative damage.
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TANG Jia, MAO Chun-li, HUANG Qi, TANG Jun, SONG Yu-mei, SUN Ming, MAO Pei-sheng. The Antioxidative Function Changes in Embryonic Mitochondria of Elymus sibiricus Seeds of Different Storage Years. Acta Agrestia Sinica, 2020, 28(5): 1226-1232.
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