草地学报

›› 2004, Vol. 12 ›› Issue (2): 120-123.DOI: 10.11733/j.issn.1007-0435.2004.02.009

• 研究论文 • 上一篇    下一篇

攀西地区野生狗牙根遗传多样性研究

王赞1,2, 毛凯1, 吴彦奇1, 高洪文3, 韩建国2   

  1. 1. 四川农业大学草学系, 雅安, 625014;
    2. 中国农业大学草地所, 北京, 100094;
    3. 中国农业科学院畜牧所, 北京, 100094
  • 收稿日期:2003-10-23 修回日期:2004-03-15 出版日期:2004-05-15 发布日期:2004-05-15
  • 作者简介:王赞(1974- ),男,汉族,内蒙古磴口县人,中国农业大学在读博士研究生,主要从事牧草及草坪草种质资源研究

Assessment of Genetic Diversity of Native Bermudagrass Accessions Collected in Panxi Region,Sichuan Province

WANG Zan1,2, MAO Kai1, WU Yan-qi1, GAO Hong-wen3, HANG Jian-guo2   

  1. 1. Department of Grassland Science, Sichuan Agriculture University, Ya’an, Sichuan province 625014, China;
    2. Institute of Grassland Science, China Agriculture University, Beijing 100094, China;
    3. Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing, 100094, China
  • Received:2003-10-23 Revised:2004-03-15 Online:2004-05-15 Published:2004-05-15

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摘要： 从形态、同工酶水平研究15份狗牙根(其中野生种12份,对照3份)的遗传多样性。结果表明:野生种外部形态变异较大,聚类分析结果可分为3类;同工酶电泳结果显示野生种蛋白质多样性程度较高;不同居群蛋白质图谱与地理分布和生境条件有一定关系;过氧化物酶同工酶作为遗传标记,可用于本地区狗牙根种以下单位亲源关系和遗传多样性的研究。

关键词: 草原学, 野生狗牙根, 形态性状, 同工酶, 遗传多样性

Abstract: To analyze the genetic diversity of native bermudagrass (Cynodon dactylon) by the plant morphology and isozyme levels, we collected from Panxi region 12 native bermudgrass accessions and 3 introduced accessions as controls for the study.The result shows that: (1) The 12 native accessions vary distinctly in form and structure;(2) By cluster analysis, the 15 accessions could be classified into 3 types: tall and strong; low and hairy, and fine texture with short internodes. Tifway, an introduced accession, is the only one of this type;(3) The pattern of isozyme POD electrophoresis shows that the native accessions boast striking protein polymorphism which is related to their geographic distribution and ecological factors. POD isozyme, as a genetic mark, could be used to attest to the relationship and genetic diversity of burmudagrass accessions collected in the same area.

Key words: Grassland science, Native bermudagrass (Cynodon dactylon), Morphological trait, Isozyme, Genetic diversity

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