草地学报

›› 2005, Vol. 13 ›› Issue (1): 43-46.DOI: 10.11733/j.issn.1007-0435.2005.01.011

• 研究论文 • 上一篇    下一篇

中国假俭草居群遗传多样性研究Ⅱ:三种等位酶证据

宣继萍, 刘建秀   

  1. 江苏省中国科学院植物研究所中山植物园, 南京, 210014
  • 收稿日期:2003-10-30 修回日期:2004-12-08 出版日期:2005-02-15 发布日期:2005-02-15
  • 通讯作者: 刘建秀
  • 作者简介:宣继萍(1976- ),女,浙江东阳人,助理研究员,硕士,主要从事暖季型草坪草种质资源的遗传多样性研究
  • 基金资助:
    国家自然科学基金项目“中国假俭草种质资源遗传多样性研究”(30070538)资助

Study on the Heredidy Diversity of China Native Centipedegrass Population (Ⅱ):Evidence of Alleozyme

XUAN Ji-ping, LIU Jian-xiu   

  1. Zhongshan Arboretum, Institute of Botany, Chinese Academy of Sciences of Jiangsu Province, Nanjing, Jiangsu Province 210014, China
  • Received:2003-10-30 Revised:2004-12-08 Online:2005-02-15 Published:2005-02-15

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摘要： 选择产自我国不同地域的6个假俭草(Eremochloa ophiuroides (Munro) Hack)居群,利用垂直平板丙烯酰胺凝胶电泳技术分析了过氧化物同工酶(POD)、酯酶同工酶(EST)和苹果酸脱氢酶同工酶(MDH)酶谱的9个等位酶位点.结果表明:中国假俭草的基因多样性为0.336,其中居群内为0.176,占总变异的52.4%,居群间为0.16,占总变异的47.6%;居群间遗传一致度为0.945～1.000,其中连云港和贵州2个居群间的遗传一致度明显低于其余4个;中国假俭草居群内的遗传多样性大于居群间遗传多样性,种内遗传差异很小.

关键词: 假俭草, 居群, 等位酶, 遗传多样性

Abstract: The nine loci of peroxidase isozymes(POD), esterase(EST), and malate dehydrogenase(MDH) in 6 populations of centipedegrass(Eremochloa ophiuroides(Munro)Hack.)collected from different areas of mainland China were detected and analyzed with polyacrylamide gel electrophoresis method.The results show that the gene diversity of the centipedegrass is 0.336, of which 0.176 is within the populations, amounting to 52.4% of the total; (0.16) is among the populations, taking 47.6% of the total. Genetic similarity among the centipede-grass populations reaches 0.945~1.000. The similarity between the Lianyungang population and Guizhou population is slighter than among the other 4 populations. The results illustrate that the genetic diversity within a population is more notable than that among the populations in Chinese-native centipedegrass, while the genetic difference of the centipedegrass species is very slight.

Key words: Centipedegrass, Population, Alleozyme, Genetic diversity

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