SRAP分子标记在假俭草杂交后代真实性鉴定中的应用

doi:10.11733/j.issn.1007-0435.2009.02.001

›› 2009, Vol. 17 ›› Issue (2): 135-140.DOI: 10.11733/j.issn.1007-0435.2009.02.001

• 研究报告 • 下一篇

SRAP分子标记在假俭草杂交后代真实性鉴定中的应用

郑轶琦¹, 宗俊勤², 薛丹丹¹, 陈宣², 刘建秀²

1. 南京农业大学园艺学院, 中国, 南京, 210095;
2. 江苏省中科院植物所, 中国, 南京, 210014

收稿日期:2008-05-28 修回日期:2008-09-08 出版日期:2009-04-15 发布日期:2009-04-15
通讯作者: 刘建秀, E-mail:turfunit@yahoo.com.cn
作者简介:郑轶琦(1977- ), 女, 河南洛阳市人, 讲师, 博士研究生, 研究方向为假俭草种质资源及遗传育种, E-mail:botanyzyq@yahoo.com.cn
基金资助:
国家自然科学基金(30670200)资助

Application of SRAP Markers to the Identification of Eremochloa ophiuroides(Munro)Hack Hybrids

ZHENG Yi-qi¹, ZONG Jun-qin², XUE Dan-dan¹, CHEN Xuan², LIU Jian-xiu²

1. College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China;
2. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province, 210014, China

Received:2008-05-28 Revised:2008-09-08 Online:2009-04-15 Published:2009-04-15

摘要/Abstract

摘要： 以假俭草(Eremochloa ophiuroides(Munro)Hack)两个杂交组合的4个亲本(E022、E142、E102、E092(1))及其杂交后代为材料,从400对SRAP引物组合中筛选出具有父本特征带的引物,选取其中带型稳定、清晰的引物组合进行杂种真实性鉴定。结果表明:(1)A(E102×E092(1))组合的两个亲本间具有父本特征带的引物组合共有51对,B(E142×E022)组合的两个亲本间具有父本特征带的引物组合共有87对。(2)应用6对SRAP引物(303、297、283、180、332和358)对A(E102×E092(1))组合进行杂种真实性鉴定,其中真杂种数目为89个;应用4对SRAP引物(18、199、221和288)对B(E142×E022)组合的杂交后代进行真实性鉴定,其中真杂种的数目是83个。上述鉴定的假俭草真杂种可以用于构建假俭草遗传连锁图谱及重要性状的QTL定位等研究。

关键词: 假俭草, 杂种鉴定, SRAP标记, 父本特征带

Abstract: The hybrids status from two hybridization combinations of Eremochloa ophiuroides(Munro)Hack with four parents(E022,E142,E102,E092(1))were tested using SRAP primer combinations with clear and stabilized paternal characteristic bands selected from 400 primer combinations.The results show:(1)51 primer combinations with paternal characteristic bands were selected in A(E102×E092(1))and 87 primer combinations were selected in B(E142×E022).(2)Six primer combinations(303,297,283,180,332 and 358)were used to identify the hybrids status in A(E102×E092(1))and of which,89 were true hybrids.Four primer combinations(18,199,221 and 288)were used to identify the hybrids status in B(E142×E022)and of which,83 were true hybrids.These true hybrids could be useful in the genetic linkage map construction and QTL mapping of important characters.

Key words: Eremochloa ophiuroides(Munro)Hack, Hybrid identification, SRAP markers, Paternal characteristic band

中图分类号:

S336

郑轶琦, 宗俊勤, 薛丹丹, 陈宣, 刘建秀. SRAP分子标记在假俭草杂交后代真实性鉴定中的应用[J]. , 2009, 17(2): 135-140.

ZHENG Yi-qi, ZONG Jun-qin, XUE Dan-dan, CHEN Xuan, LIU Jian-xiu. Application of SRAP Markers to the Identification of Eremochloa ophiuroides(Munro)Hack Hybrids[J]. , 2009, 17(2): 135-140.

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参考文献

[1] 刘学诗,刘建秀,郭海林.华东地区假俭草种质资源多样性初步研究3:染色体倍性和形态[J]. 草业学报,2003,12(3):90-94
[2] Bouton J H.Plant breeding characteristics relating to improvement of centipedegrass[J]. Soil and Crop Science Society of Florida Proceedings,1983,42:53-58
[3] Hanna W W,Burton G W.Cytology,reproductive behavior and fertility characteristics of centipedegrass[J]. Crop Science,1978,18:835-837
[4] 李西,毛凯.假俭草研究概况[J]. 草业科学,2000,17(5):13-17
[5] 郑玉红,刘建秀.假俭草(Eremochloa ophiuroides(Munro.)Hack.)种质资源改良进展[J]. 植物学通报,2004,21(5):587-594
[6] 柳后起,周守标,谢传俊.假俭草种植资源研究进展[J]. 草业科学,2008,25(1):59-65
[7] Paulo J E,Edite M J,Jose M L.Identification of mandarin hybrids by isozyme and RAPD analysis[J]. Seientia Horticulturae,1999,81:287-299
[8] 郭海林,刘建秀,朱雪花,等.结缕草植物杂交育种及其杂种鉴定一同工酶的变异分析[J]. 草业学报,2006,15(6):101-108
[9] 李小雷,于卓,贺鹏程,等.加拿大披碱草、肥披碱草及其杂种F1 RAPD分析[J]. 草地学报,2008,16(1):23-27
[10] Olaf W,Pedro S G,Juan G,et al.Identification of Pistacia×saportae Burnat(Anacardiaceae)by RAPD analysis and morphological characters[J]. Scientia Horticulturae,2001,91:179-186
[11] Masumi Y,Hiromi A.Michiharu N,et al.PCR-based molecular markers in Asiatic hybrid lily[J]. Scientia Horticuhurae,2002,96:225-234
[12] Hulya I.RAPD markers assisted varietal identification and genetic purity test in pepper,Capsicum annuum[J]. Scientia Horticulturae,2003,97:211-218
[13] Liu L W,Wang Y,Gong Y Q,et al.Assessment of genetic purity of tomato(Lycopersicon esculentum L.)hybrid using molecular markers[J]. Scientia Horticuhurae,2007,115:7-12
[14] 罗玉兰,张冬梅.SSR标记及形态性状鉴定红刺玫和月季F1后代[J]. 分子植物育种,2007,5(6):839-842
[15] Wang J,Zhong G Y,Register J C,et al.Identification of parents of F1 hybrids through SSR profiling of maternal and hybrid tissue[J]. Euphytica,2002,124:29-34
[16] 鹿金颖,毛永民,申莲英,等.用AFLP分子标记鉴定冬枣自然授粉实生后代杂种的研究[J]. 园艺学报,2005,32(4):680-683
[17] 阮成江,钦佩,韩睿明.等.AFLP分子标记在签定海滨锦葵杂交后代上的应用[J]. 南京林业大学学报(自然科学版),2005,29(1):20-24
[18] Li G,Quiros C F.Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics,2001,103:455-461
[19] 王刚,潘俊松,李效尊,等.黄瓜SRAP遗传连锁图的构建及侧枝基因定位[J]. 中国科学C辑:生命科学,2004,34(6):510-516
[20] 潘俊松,王刚,李效尊,等.黄瓜SRAP遗传连锁图的构建及始花节位的基因定位[J]. 自然科学进展,2005,15(2):167-172
[21] 吴洁,谭文芳,何俊蓉,等.甘薯SRAP连锁图构建淀粉含量QTL检测[J]. 分子植物育种,2005,3(6):841-845
[22] Lin Z,He D,Zhang X.Linkage map construction and mapping QTL for cotton fibre quality using SRAP,KSR and RAPD[J]. Plant Breeding,2005,124:180-187
[23] Okazaki K,Sakamoto K,Kikuchi R,et al.Mapping and characterization of FLC homologs and QTL analysis of flowering time in Brassica oleracea[J]. Theoretical and Applied Genetics,2007,114:595-608
[24] Sun Z D,Wang Z,Tu J X.et al.An ultradense genetic recombination map for Brassica napus,consisting of 13551 SRAP markers[J]. Theoretical and Applied Genetics,2007,114:1305-1317
[25] 姜树坤,马慧.刘君,等.利用SRAP标记分析玉米遗传多样性[J]. 分子植物育种,2007,5(3):412-416
[26] 文雁成,王汉中,沈金雄,等.用SRAP标记分析中国甘蓝型油菜品种的遗传多样性和遗传基础[J]. 中国农业科学,2006,39(2):246-256
[27] Riaz A,Li G,Quresh Z,et al.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J]. Plant Breeding,2001,120:411-415
[28] Ferrio M,Pic(o)B,Nuez F.Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers[J]. Theoretical and Applied Genetics,2003,107:271-282
[29] Budak H,Shearman R C,Parmaksiz I,et al.Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers[J]. Theoretical and Applied Genetics,2004,108:328-334
[30] 王燕,龚义勤,赵统敏,等.番茄SRAP-PCR体系优化与品种分子鉴定[J]. 南京农业大学学报,2007,30(1):23-29
[31] 李丽.郑晓鹰,柳李旺.用SRAP标记分析黄瓜品种遗传多样性及鉴定品种[J]. 分子植物育种,2006,4(5):702-708
[32] Hale A L,Farnham M W,Nzaramba M N,et al.Heterosis for horticultural traits in broccoli[J]. Theoretical and Applied Genetics,2007,115:351-360
[33] 刘捷,唐章林,刘列钊,等.甘蓝型油菜遗传距离与杂种表现和杂种优势相关性分析[J]. 西南农业学报,2006,19(4):555-559
[34] Li G,Gao M,Yang B,et al.Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping[J]. Theoretical and Applied Genetics,2003,107:168-180
[35] Budak H,Shearman R C,Parmaksiz I,et al.Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs,SSRs,RAPDs,and SRAPs[J]. Theoretical and Applied Genetics,2004,109:280-288
[36] 易杨杰,张新全,黄琳凯,等.野生狗牙根种质遗传多样性的SRAP研究[3]. 遗传,2008,30(1):94-100
[37] 郭海林,刘建秀,周志芳,等.结缕草属植物种间关系和遗传多样性的SSR标记分析[J]. 草地学报,2008,16(4):552-558

SRAP分子标记在假俭草杂交后代真实性鉴定中的应用

Application of SRAP Markers to the Identification of Eremochloa ophiuroides(Munro)Hack Hybrids

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